Agilent Technologies 1290 Infinity LC System System Manual And Quick Reference page 44

3
Optimization of the Agilent 1290 Infinity LC System
How to Configure the Optimum Delay Volume
Table 6
Flow
Rate
(ml/min)
1.5
2.0
3.0
4.0
5.0
Table 7
Column
Diameter
(mm)
2.1
3.0
4.0
4.6
For very fast gradients over 0.5 min, which can only be achieved in the Agilent
1290 Infinity Binary LC System, the delay volume of the system can be easily
reduced without changing the physical configuration of the system. This
change is achieved by changing the behavior of the autosampler.
The 80 µl delay volume of the Agilent 1290 Infinity Autosampler is due to the
flow path from the injection valve through the metering device, needle, needle
seat and connecting capillaries back to the injection valve (see
page 32). To make an injection the valve switches from mainpass to bypass so
that the metering device can draw the sample into the needle capillary. The
44
System delay times for gradient to reach head of column
System Delay Volume (microliters)
0.01 0.04 0.06
0.01 0.03 0.04
0.01 0.02 0.03
0.01 0.01 0.02
0.00 0.01 0.02
Approximate volume of liquid in typical column dimensions assuming that
porosity = 0.6
30
Column Volume - Liquid Phase (microliters)
62
127
226
299
Agilent 1290 Infinity LC System Manual and Quick Reference
0.08 0.24
0.06 0.18
0.04 0.12
0.03 0.09
0.02 0.07
Column Length (mm)
50 100
104 208
212 424
377 754
499 997
0.29 0.34 0.39
0.22 0.26 0.29
0.14 0.17 0.19
0.11 0.13 0.15
0.09 0.10 0.12
150 250
312 520
636 1060
1131 1885
1496 2493
Figure 18 on