Agilent Technologies 1290 Infinity LC System System Manual And Quick Reference page 120

6
Appendix
Setting Up a Method using Edit Entire Method
Vials should not have a septum i.e., they should be open to avoid transfer of carryover
N O T E
material on the septum.
• Stop Time / Post Time are set to No Limit / Off and these values are taken care
• Advanced - Auxiliary
• Advanced - High Throughput
120
• Location determines which vial or well plate will be used, if Wash Vial was
selected.
• Repeat determines, if Wash Vial was selected, how many times the needle
dips into the vial (default 3, maximum 5).
of in the pump tab.
• Draw speed is the speed that the sample is pulled into the needle. Default
value is 100 µl/min. It should be slowed down for viscous samples or for
greater precision with small sample volumes (<2 µL).
• Eject speed is the speed of delivery from the needle.
• Draw position is the vertical offset from the nominal injection position of
10 mm above the bottom of a vial. This is about half-way up a 2 ml vial so
should be given a negative offset to take the sample from near the bottom
of the vial, for example, a value of -7 mm would place the tip of the
needle 3 mm above the bottom of the vial.
• Equilibration time is a delay time between drawing the sample and moving
the needle.
• Sample flush out factor determines how long the autosampler would wait
after injection before allowing the valve to turn to bypass. This ensures
that the sample zone has cleared from the needle, seat and injection
valve. Default value is 5.
• Vial/Well bottom sensing is an alternative to using draw position offset.
The needle slowly moves down until it touches the bottom of the vial or
well and then lifts up 1 mm. This is a versatile way of ensuring the needle
is close to the bottom of the vial but it takes slightly longer to complete
the injection and should not be used if there are particulates at the
bottom of the vial which might block the needle.
• Automatic delay volume reduction (ADVR) switches the injection valve from
mainpass to bypass after the injection has taken place and a volume
defined by the sample flush out factor has swept through the injector.
Agilent 1290 Infinity LC System Manual and Quick Reference