Extra-Column Volume - Agilent Technologies 1290 Infinity LC System System Manual And Quick Reference

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Optimization of the Agilent 1290 Infinity LC System
Delay Volume and Extra-Column Volume
times of about one minute. However, the delay volume must be reduced further
to achieve delay times which are a fraction of the intended run time. This is
achieved with the Agilent 1290 Infinity LC System due to the low delay volume
of the pump flow path, low volume of the Jet Weaver mixer and low-volume of
the flow path through the autosampler.

Extra-Column Volume

Extra-column volume is a source of peak dispersion that will reduce the
resolution of the separation and so should be minimized. Smaller diameter
columns require proportionally smaller extra-column volumes to keep peak
dispersion at a minimum.
In a liquid chromatograph the extra-column volume will depend on the
connection tubing between the autosampler, column and detector; and on the
volume of the flow cell in the detector. The extra-column volume is minimized
with the Agilent 1290 Infinity LC System due to the narrow-bore (0.12 mm
i.d.) tubing, the low-volume heat exchangers in the column compartment and
the Max-Light cartridge cell in the detector.
Agilent 1290 Infinity LC System Manual and Quick Reference 41