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Agilent Technologies 1290 Infinity Flexible Cube User Manual
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Agilent Technologies 1290 Infinity Flexible Cube User Manual

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Agilent 1290 Infinity
Flexible Cube
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Summary of Contents for Agilent Technologies 1290 Infinity Flexible Cube

  • Page 1 Agilent 1290 Infinity Flexible Cube User Manual Agilent Technologies...
  • Page 2 (June 1987) or any equivalent agency regu- lation or contract clause. Use, duplication or disclosure of Software is subject to Agi- lent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will 1290 Infinity Flexible Cube User Manual...
  • Page 3 In This Guide... In This Guide... This manual covers the Agilent 1290 Infinity Flexible Cube (G4227A). 1 Introduction This chapter gives an introduction to the module, instrument overview and internal connectors. 2 Site Requirements and Specifications This chapter provides information on environmental requirements, physical and performance specifications.
  • Page 4 This chapter provides information on cables used with the 1290 series of HPLC modules. 13 Hardware Information This chapter describes the module in more detail on hardware and electronics. 14 Appendix This chapter provides addition information on safety, legal and web. 1290 Infinity Flexible Cube User Manual...
  • Page 5 Flow Connections to the Module for Lowest Carryover Installing the Valve Heads 4 LAN Configuration LAN-Configuration 5 Using the Module Instrument Configuration Setting up the Flexible Cube with Agilent ChemStation Solvent Information Algae Growth in HPLC Systems 1290 Infinity Flexible Cube User Manual...
  • Page 6 Overview of Maintenance Cleaning the Module Exchange Flush Pump Inlet Valve Exchange Flush Pump Outlet Valve Exchange Valve Rotor Seal Replacing Parts of the Valve Head Replacing Valve Heads Installing the Capillaries Replacing Module Firmware 1290 Infinity Flexible Cube User Manual...
  • Page 7 CAN/LAN Cables External Contact Cable Agilent Module to PC Agilent 1200 Module to Printer 13 Hardware Information Firmware Description Electrical Connections Interfaces Setting the 8-bit Configuration Switch (without On-board) LAN Instrument Layout Early Maintenance Feedback 1290 Infinity Flexible Cube User Manual...
  • Page 8 Contents 14 Appendix General Safety Information The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Radio Interference Sound Emission Agilent Technologies on Internet 1290 Infinity Flexible Cube User Manual...
  • Page 9 1290 Infinity Flexible Cube User Manual Introduction Features Overview of the Module Flexible Cube Technical Capability Valve solution / Valve technique Dual and Multi Column Selection Sample Enrichment and Sample Cleanup Alternating Column Regeneration Solvent Selection Bio-inert Materials This chapter gives an introduction to the module, instrument overview and internal connectors.
  • Page 10 Autosampler. For specifications, see “Performance Specifications” on page 34. This 1290 Infinity Flexible Cube has been introduced as part of the Agilent 1290 Infinity N O T E Liquid Chromatograph. 1290 Infinity Flexible Cube User Manual...
  • Page 11 Overview of the Module Overview of the Module The Agilent 1290 Infinity Flexible Cube comprises a flush pump that can deliver flow between 1 and 4 mL/min from 3 different solvent bottles. The system also includes slots so you can have one or, optionally, two valve drives.
  • Page 12 The needle wash port and the needle seat back flushing function may generate large N O T E amount of waste. Make sure to have the waste tubing installed in the autosampler and a waste container with sufficient capacity available. 1290 Infinity Flexible Cube User Manual...
  • Page 13 1 Mainpass Figure 1 Mainpass The analytical pump is connected to the autosampler sampling loop, and the mobile phase is flowing through the autosampler injection loop continuously and thereby cleaning the insides of the tubings. 1290 Infinity Flexible Cube User Manual...
  • Page 14 2 Switch to Bypass Figure 2 Switch to Bypass The injection valve is switched to bypass, removing the injection loop from the high pressure flow path and connecting the flush pump with the needle seat. 1290 Infinity Flexible Cube User Manual...
  • Page 15 The first cleaning solvent available for flushing is normally of a high organic concentration with appropriate modifiers. This solvent should be able to dissolve most components of the sample, and thereby remove them from the Autosampler flow path. 1290 Infinity Flexible Cube User Manual...
  • Page 16 Figure 4 Flush with dissolving solvent #2 (B2) If this is not sufficient to reduce the carryover a dissolving solvent #2 may be used with either different modifiers and/or a really strong cleaning solvent. 1290 Infinity Flexible Cube User Manual...
  • Page 17 Any flushing solvent used must be miscible with previous solvent. If this is not the case it N O T E can result in precipitations, blockages or reduced performance of the instrument. 1290 Infinity Flexible Cube User Manual...
  • Page 18 6 Switch back to Mainpass Figure 6 Switch back to Mainpass When the complete flushing procedure has finished the autosampler switches back to Mainpass operation and thereby introduce the sample to the flow path. 1290 Infinity Flexible Cube User Manual...
  • Page 19 Quickly change between two different stationary phases to check your separation selectivity, or use two identical stationary phases to have the second column immediately available after the first one loses efficiency, for example with complex matrices. 1290 Infinity Flexible Cube User Manual...
  • Page 20 The multi-column setup might also be used, if several operators are sharing system. Figure 7 Multiple Column Selection (Example of Schematic Setup for 6-Column Selector Valve Head) 1290 Infinity Flexible Cube User Manual...
  • Page 21 Before injection into an LC or LC/MS system, the sample matrix must be separated from the analytes of interest. Otherwise, contaminants can disrupt separation and detection or even damage the analytical column. 1290 Infinity Flexible Cube User Manual...
  • Page 22 Introduction Valve solution / Valve technique Sample Enrichment Sample Cleanup 1290 Infinity Flexible Cube User Manual...
  • Page 23 After the valve switches, an additional pump backflushes the matrix components out of the pre-column to waste, while the analytes are separated on the main column. Backflushing prepares the pre-column for the next injection. 1290 Infinity Flexible Cube User Manual...
  • Page 24 Core of the alternating column regeneration is the Agilent 1200 Infinity Series 2 position/10 port valve, which allows simultaneous analysis on one column while a second identical column is flushed and equilibrated. 1290 Infinity Flexible Cube User Manual...
  • Page 25 The 12ps/13pt solvent selection valve (G4235A) can be used for solvent selection (flow rate < 10 mL/min) as illustrated in Figure 9 on page 25. It offers automated access to 12 different eluents. Figure 9 Solvent Selection (Schematic Setup) 1290 Infinity Flexible Cube User Manual...
  • Page 26 Bio-inert Materials Bio-inert Materials For the Agilent 1260 Infinity Bio-inert LC system, Agilent Technologies uses highest quality materials in the flow path (also referred to as wetted parts), which are widely accepted by life scientists, as they are known for optimum inertness to biological samples and ensure best compatibility with common samples and solvents over a wide pH range.
  • Page 27 Upstream of sample introduction: • Titanium Downstream of sample introduction: • Agilent uses stainless steel cladded PEEK capillaries, which keep the flow path free of steel and provide pressure stability up to 600 bar. 1290 Infinity Flexible Cube User Manual...
  • Page 28 Do not use any parts that are not labeled as Agilent “Bio-inert”. For solvent compatibility of these materials, see “Material Information” on page 80. 1290 Infinity Flexible Cube User Manual...
  • Page 29 1290 Infinity Flexible Cube User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications This chapter provides information on environmental requirements, physical and performance specifications. Agilent Technologies...
  • Page 30 ➔ Make sure the power connector of the instrument can be easily reached and unplugged. ➔ Provide sufficient space behind the power socket of the instrument to unplug the cable. 1290 Infinity Flexible Cube User Manual...
  • Page 31 Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Absence of ground connection WA R N I N G The absence of ground connection can lead to electric shock or short circuit.
  • Page 32 Do not store, ship or use your module under conditions where temperature fluctuations could cause condensation within the module. ➔ If your module was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation. 1290 Infinity Flexible Cube User Manual...
  • Page 33 Operating altitude Up to 2000 m (6562 ft) Non-operating altitude Up to 4600 m (15092 ft) For storing the module Safety standards: Installation category II, Pollution degree 2 For indoor use only. IEC, CSA, UL 1290 Infinity Flexible Cube User Manual...
  • Page 34 G4226A sampler Pressure range of flush Up to 50 bar pump Flow rate of flush pump Up to 4 mL/min Number of solvents Up to 3 solvents Sample viscosity range 0.2 – 5 cp 1290 Infinity Flexible Cube User Manual...
  • Page 35 LC/CE Driver Version A.02.06 Local control Agilent Instant Pilot (G4208A) B.02.08 or later Communications Controller-area network (CAN), RS-232C, APG Remote: ready, start, stop and shut-down signals, optional four external contact closures and BCD vial number output. 1290 Infinity Flexible Cube User Manual...
  • Page 36 MS-detection: Sample : 50 ng/μl chlorhexidine (dissolved in mobile phase A), 1 μL injected and measured on Agilent 6460 QQQ (in specified conditions); MRM 1: 505.5 → 170 (CE: 36 V); MRM 3: 505.5 → 201.2 (CE: 20 V); fragmentor: 150 V, delta EMV(+): 200 V 1290 Infinity Flexible Cube User Manual...
  • Page 37 10-32 PEEK, special 0-14 head, bio-inert Ceramic incompatible with some mineral acids. For more information see Solvent Information. pre-column volume G4234A/C: 0.37 µL, G5639A: 0.81 µL; post column volume G4234A/C: 0.50µL, G5639A: 1.33 µL 1290 Infinity Flexible Cube User Manual...
  • Page 38 Site Requirements and Specifications Performance Specifications 1290 Infinity Flexible Cube User Manual...
  • Page 39 1290 Infinity Flexible Cube User Manual Installing the Module Unpacking the Module Optimizing the Stack Configuration One Stack Configuration Supported Two Stack Configurations Installing the Module Flow Connections to the Module for Lowest Carryover Installing the Valve Heads This chapter gives information about the preferred stack setup for your system and the installation of your Flexible Cube.
  • Page 40 Unpacking the Module Damaged Packaging If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. "Defective on arrival" problems C AU T I O N If there are signs of damage, please do not attempt to install the module.
  • Page 41 Flexible Cube Accessory Kit (see “Flexible Cube Accessory Kit” on page 155) Second 1290 Infinity Flexible Cube Generic Valve Drive optional For the valve drive the respective valve heads need to be ordered in addition. N O T E 1290 Infinity Flexible Cube User Manual...
  • Page 42 Chromatograph, you can ensure optimum performance by installing the following configurations. These configurations optimize the system flow path, ensuring minimum delay volume. For other possible configurations, please refer to the Agilent 1290 Infinity System Manual. 1290 Infinity Flexible Cube User Manual...
  • Page 43 Installing the Module Optimizing the Stack Configuration One Stack Configuration Figure 10 Supported One Stack Configuration (Front View) 1290 Infinity Flexible Cube User Manual...
  • Page 44 Installing the Module Optimizing the Stack Configuration Figure 11 Supported One Stack Configuration (Rear View) 1290 Infinity Flexible Cube User Manual...
  • Page 45 Installing the Module Optimizing the Stack Configuration Supported Two Stack Configurations Two Stack Configuration (Standard) Figure 12 Supported Two Stack Configuration Front View (Standard) 1290 Infinity Flexible Cube User Manual...
  • Page 46 Installing the Module Optimizing the Stack Configuration Figure 13 Supported Two Stack Configuration Rear View (Standard) 1290 Infinity Flexible Cube User Manual...
  • Page 47 Installing the Module Optimizing the Stack Configuration Two Stack Configuration (Thermostatted) Figure 14 Supported Two Stack Configuration Front View (Thermostatted) 1290 Infinity Flexible Cube User Manual...
  • Page 48 Installing the Module Optimizing the Stack Configuration Figure 15 Supported Two Stack Configuration Rear View (Thermostatted) 1290 Infinity Flexible Cube User Manual...
  • Page 49 Do not connect the power cable to the Instrument while the covers are removed. 1 Place the Flexible Cube in the stack, see “Optimizing the Stack Configuration” on page 42. 2 Ensure the line power switch on the front of the module is OFF (switch stands out). 1290 Infinity Flexible Cube User Manual...
  • Page 50 The module is turned on when the line power switch is pressed and the green indicator N O T E lamp is illuminated. The module is turned off when the line power switch is protruding and the green light is off. 1290 Infinity Flexible Cube User Manual...
  • Page 51 This procedure shows the Flexible Cube outside of a system. In an Agilent 1290 Infinity N O T E Liquid Chromatograph, the Flexible Cube is located between a G4220A Binary pump (below) and the G4226A Autosampler (above), see “Optimizing the Stack Configuration” on page 42. 1290 Infinity Flexible Cube User Manual...
  • Page 52 Install the bottle head assemblies into the solvent Install low restriction needle seat from accessory kit into selection valve of the Flexible Cube. autosampler (see autosampler manual - Maintenance section for procedure). 1290 Infinity Flexible Cube User Manual...
  • Page 53 (do not use any tools). Be sure that the guide pin snaps into the groove of the valve drive thread. Removing the Valve Dummy 1 To remove the valve dummy, loosen the nut manually. 1290 Infinity Flexible Cube User Manual...
  • Page 54 When using multiple column compartments as part of a method development solution, make sure that the high pressure valve head is connected to the autosampler and the low pressure valve head is connected to the detector. 1290 Infinity Flexible Cube User Manual...
  • Page 55 To have the valve correctly recognized by the Flexible Cube you must have the module N O T E powered off for at least 10 s. 1290 Infinity Flexible Cube User Manual...
  • Page 56 The outside pin (red) must fit into the outside the valve you should feel some resistance from the drive. groove. Otherwise the pins on the valve shaft are not properly fitted into the corresponding adapters on the valve drive. 1290 Infinity Flexible Cube User Manual...
  • Page 57 Fasten the nut manually. Do not use any tools. Power on or power-cycle your module, so the valve head gets recognized during module initialization. N O T E Power Off the Infinity valve drive for at least 10 s. 1290 Infinity Flexible Cube User Manual...
  • Page 58 Installing the Module Installing the Valve Heads 1290 Infinity Flexible Cube User Manual...
  • Page 59 1290 Infinity Flexible Cube User Manual LAN Configuration LAN-Configuration This chapter provides information on connecting the module to the Agilent Data System. Agilent Technologies...
  • Page 60 The G1369C CAN must connect to CAN port of the module that hosts the LAN card N O T E (typically the detector). Then the hosted module CAN must connect to free CAN port of the system. All other combinations will not work. 1290 Infinity Flexible Cube User Manual...
  • Page 61 1290 Infinity Flexible Cube User Manual Using the Module Instrument Configuration Setting up the Flexible Cube with Agilent ChemStation The Flexible Cube User Interface The Flexible Cube User Interface in Autosampler GUI Control Settings Method Parameter Settings Module Configuration Solvent Information...
  • Page 62 Using the Module Instrument Configuration Instrument Configuration Figure 17 Instrument configuration 1290 Infinity Flexible Cube User Manual...
  • Page 63 2 Choose your valve drive(s)/valve head(s) from the list of configurable Valve Types. If you choose the wrong valve configuration your system will show up in offline modus. N O T E 1290 Infinity Flexible Cube User Manual...
  • Page 64 If the Autosampler is linked to the Flexible Cube you will see this additional icon in the Autosampler GUI. Figure 19 ChemStation Method & Run Control - Flexible Cube GUI 1290 Infinity Flexible Cube User Manual...
  • Page 65 Using the Module Setting up the Flexible Cube with Agilent ChemStation Figure 20 ChemStation Method & Run Control - Autosampler GUI 1290 Infinity Flexible Cube User Manual...
  • Page 66 • show the Control user interface • show the Method user interface • Error Method • Identify Device • Switch Pump On • Execute Pump Command... • Left Valve Position Cluster • Right Valve Position Cluster 1290 Infinity Flexible Cube User Manual...
  • Page 67 • Autodetect: The pressure limit for the cluster is set to that of the module with the lowest pressure limit. Right Valve/Pressure Limit Cluster: see above Illumination: Toggles the illumination of the Flexible Cube, On or Off. 1290 Infinity Flexible Cube User Manual...
  • Page 68 Stoptime: Stoptime enables you to set a time at which the Flexible Cube stops an analysis. If the Flexible Cube is used with other Agilent Modular LC modules, the Flexible Cube Stoptime stops the Flexible Cube only and does not stop any other modules. 1290 Infinity Flexible Cube User Manual...
  • Page 69 • Use current valve position: Uses the current position of the valve. • Use valve position: Specifies a valve position other than the current one. Set the valve position by clicking the down arrow and selecting a position from the drop-down list. 1290 Infinity Flexible Cube User Manual...
  • Page 70 Time field and appropriate values in the following fields of the timetable. The values in the Flexible Cube timetable change instantaneously at the time defined in the timetable. 1290 Infinity Flexible Cube User Manual...
  • Page 71 • Right valve change position: see above • Pump volume: At the specified time, the pump delivers the specified volume of solvent. • Pump for time: At the specified time, the pump is switched on for the specified time. 1290 Infinity Flexible Cube User Manual...
  • Page 72 The Duration field is available only when Pump for a specified time is selected. • Volume: Enter the required volume of solvent to be delivered (in mL) in this field. The Volume field is available only when Pump a specified volume is selected. 1290 Infinity Flexible Cube User Manual...
  • Page 73 Not Ready state. Best working practice: the master should always be the valve with the smallest number of N O T E ports. This avoids conflicts if different valve heads are used. 1290 Infinity Flexible Cube User Manual...
  • Page 74 • Show the Control User Interface (special module settings) • Show the Method User interface (same as via menu Instrument – Setup G4226A) • And Flexible Cube specific items: • Autoclean / Purge • Prime 1290 Infinity Flexible Cube User Manual...
  • Page 75 This feature primes the flush solvent inlet lines with fresh solvent. It can be configured what solvent lines to prime and additionally the needle wash port can also be primed with solvent. 1290 Infinity Flexible Cube User Manual...
  • Page 76 Priming the Needle wash port for a given period of time. If the Flexible Cube is linked with the pump, the flush pump and the solvent selection valve N O T E are blocked for other usage. 1290 Infinity Flexible Cube User Manual...
  • Page 77 Enable needle seat back flush has to be checked. Flexible Cube settings The setup includes choosing what solvent to use. Note that the Start Cond. (A1) cannot be deselected. Additionally the flow rate and duration can be selected. 1290 Infinity Flexible Cube User Manual...
  • Page 78 Do not leave the Autosampler to Flexible Cube capillary installed if the Flexible Cube is not part of the Software configuration or if turned off during operation. These settings are available via menu Instrument > More 1290 Infinity ALS > Control Configuration. 1290 Infinity Flexible Cube User Manual...
  • Page 79 Setting up the Flexible Cube with Agilent ChemStation Options: Lists installed options for the G4226A Autosampler. Flexible Cube installed: If the Flexible Cube option is checked the Flexible Cube cleaning feature becomes activated in the Method and Configuration screens. 1290 Infinity Flexible Cube User Manual...
  • Page 80 Most data available refers to room temperature (typically 20 – 25 °C, 68 – 77 °F). If corrosion is possible, it usually accelerates at higher temperatures. If in doubt, please consult technical literature on chemical compatibility of materials. 1290 Infinity Flexible Cube User Manual...
  • Page 81 G7104C and for normal phase applications in 1260 Infinity pumps. Polyethylene has a good stability for most common inorganic solvents including acids and bases in a pH range of 1 to 12.5. It is compatible with many 1290 Infinity Flexible Cube User Manual...
  • Page 82 For example, a 1 % solution of acetic acid in methanol will attack steel. • Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid). • Mixtures of carbon tetrachloride with 2-propanol or THF. 1290 Infinity Flexible Cube User Manual...
  • Page 83 It can be corroded by complexing cyanides and concentrated acids like aqua regia. Zirconium Oxide (ZrO Zirconium Oxide is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. 1290 Infinity Flexible Cube User Manual...
  • Page 84 DMF (dimethyl formamide). Sapphire, Ruby and Al -based ceramics Sapphire, ruby and ceramics based on aluminum oxide Al are inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. 1290 Infinity Flexible Cube User Manual...
  • Page 85 • Use the amber solvent bottle ( Solvent bottle, amber, 1000 mL (9301-6526)) supplied with the instrument for your aqueous mobile phase. • If possible add a few mg/L sodium azide or a few percent organic solvent to the aqueous mobile phase. 1290 Infinity Flexible Cube User Manual...
  • Page 86 Using the Module Algae Growth in HPLC Systems 1290 Infinity Flexible Cube User Manual...
  • Page 87 1290 Infinity Flexible Cube User Manual Optimizing Lab Performance with Flexible Cube Preparing the Autosampler and Flexible Cube How to Achieve Lowest Carryover Internal Needle Wash External Needle Wash Needle Seat Back Flush Injection Valve Rinse How to Achieve the Highest Throughput Using the Flexible Cube...
  • Page 88 • Use separate solvent bottle for external needle wash in Autosampler. • Use inlet filters for flush solvent bottles. • It is recommended to sonicate solvent A1 which should be set to the chromatographic starting condition before use, to avoid generation of air bubbles. 1290 Infinity Flexible Cube User Manual...
  • Page 89 Needle seat design, improper sealing, worn out. Valve Rotor/Stator, bad design, wear. Capillary fittings misadjusted (at all connections contaminated with the sample!). Column related carryover (all fittings and connections of the column, packing, stationary phase-sample interaction). 1290 Infinity Flexible Cube User Manual...
  • Page 90 Not correctly applied fittings are the sole largest contributor to system carryover. Make N O T E sure to use correct fittings and to tighten the fittings correctly. 1290 Infinity Flexible Cube User Manual...
  • Page 91 The flush port and its peristaltic pump and tubing should be regularly flushed to ensure the lowest carryover. For example, before using the system each day, prime the flush port for three minutes with appropriate solvent. 1290 Infinity Flexible Cube User Manual...
  • Page 92 If the needle seat or rotor seal becomes contaminated it has to be back-flushed by manually changing the flow connections. This is one of the tasks that can be automated using the Flexible Cube module. Figure 23 Schematic of Autosampler with Flexible Cube Setup 1290 Infinity Flexible Cube User Manual...
  • Page 93 Any flushing solvent used must be miscible with previous solvent. If this is not the case it N O T E can result in precipitations, blockages or reduced performance of the instrument. 1290 Infinity Flexible Cube User Manual...
  • Page 94 This ensures that the bypass groove in the rotor seal of the valve contains the gradient start conditions, which is especially important when using short narrow bore columns. The Injection Valve Cleaning section allows you to specify the valve switching times anytime appropriate. 1290 Infinity Flexible Cube User Manual...
  • Page 95 You can also switch the times to off. Between the first and second, and second and third valve switches, a rinse is executed using the rinse volumes specified in the Injector Cleaning section. 1290 Infinity Flexible Cube User Manual...
  • Page 96 (EMF). Calculation of Time 1 Time 1 = Sample flush-out factor * Total flush-out volume/Flow rate Total flush-out volume = Injection volume + Seat capillary volume + Valve volume 1290 Infinity Flexible Cube User Manual...
  • Page 97 Typically a flushing time of the needle seat of about 15 s at 4 mL/min for each solvent is sufficient or at least a good starting condition. 1290 Infinity Flexible Cube User Manual...
  • Page 98 “Sample Enrichment” page 98. The valve can be conveniently located in the thermostatted column compartment or in the flexible cube. Sample Enrichment 1290 Infinity Flexible Cube User Manual...
  • Page 99 1290 Infinity pumps. For instance a 1200 Series isocratic pump would be sufficient to perform this task. The setup is illustrated below. 1290 Infinity Flexible Cube User Manual...
  • Page 100 Optimizing Lab Performance with Flexible Cube How to Achieve High Throughput 1290 Infinity Flexible Cube User Manual...
  • Page 101 1290 Infinity Flexible Cube User Manual Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Status Indicators Power Supply Indicator Module Status Indicator User Interfaces Agilent Lab Advisor Software This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces.
  • Page 102 Diagnostic Signals The module has several signals (internal temperatures, voltages and currents of lamps) that can be used for diagnosing baseline problems. These can be added like normal signals in the Agilent ChemStation software. 1290 Infinity Flexible Cube User Manual...
  • Page 103 Figure 26 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch. When the indicator is illuminated (green) the power is ON. 1290 Infinity Flexible Cube User Manual...
  • Page 104 In such a case try to re-boot the module or try a cold-start (see “Special Settings” on page 199. Then try a firmware update (see “Replacing Module Firmware” on page 150). If this does not help, a main board replacement is required. 1290 Infinity Flexible Cube User Manual...
  • Page 105 • Preferred tool should be the Agilent Lab Advisor software, see “Agilent Lab Advisor Software” on page 106. • The Agilent ChemStation may not include any maintenance/test functions. • Screenshots used within these procedures are based on the Agilent Lab Advisor software. 1290 Infinity Flexible Cube User Manual...
  • Page 106 Agilent 1200 Infinity and Agilent InfinityLab LC Series instrument. The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual. For details, refer to the Agilent Lab Advisor software help files. 1290 Infinity Flexible Cube User Manual...
  • Page 107 1290 Infinity Flexible Cube User Manual Error Information What Are Error Messages General Error Messages Timeout Shutdown Remote Timeout Lost CAN Partner Leak Sensor Short Leak Sensor Open Compensation Sensor Open Compensation Sensor Short Fan Failed Leak Open Cover Cover Violation...
  • Page 108 In all cases, error propagation is done via the CAN bus or via an APG/ERI remote cable (see documentation for the APG/ERI interface). 1290 Infinity Flexible Cube User Manual...
  • Page 109 Check the logbook for the occurrence and A not-ready condition was present during a source of a not-ready condition. Restart the sequence or multiple-injection run for a analysis where required. period longer than the timeout threshold. 1290 Infinity Flexible Cube User Manual...
  • Page 110 Check the vacuum degasser for an error The degasser failed to generate sufficient condition. Refer to the Service Manual for the vacuum for solvent degassing. degasser or the pump that has the degasser built-in. 1290 Infinity Flexible Cube User Manual...
  • Page 111 • Ensure all CAN cables are installed correctly. Exchange the CAN cable. Defective CAN cable. Switch off the system. Restart the system, and Defective main board in another module. determine which module or modules are not recognized by the system. 1290 Infinity Flexible Cube User Manual...
  • Page 112 Leak sensor not connected to the main representative. board. Please contact your Agilent service Defective leak sensor. representative. Please contact your Agilent service Leak sensor incorrectly routed, being representative. pinched by a metal component. 1290 Infinity Flexible Cube User Manual...
  • Page 113 If the resistance across the sensor falls below the lower limit, the error message is generated. Probable cause Suggested actions Please contact your Agilent service Defective main board. representative. 1290 Infinity Flexible Cube User Manual...
  • Page 114 This changes the resistance of the leak sensor which is sensed by the leak-sensor circuit on the main board. Probable cause Suggested actions Ensure all fittings are tight. Loose fittings. Exchange defective capillaries. Broken capillary. 1290 Infinity Flexible Cube User Manual...
  • Page 115 Probable cause Suggested actions Please contact your Agilent service The top foam was removed during representative. operation. Please contact your Agilent service Foam not activating the sensor. representative. 1290 Infinity Flexible Cube User Manual...
  • Page 116 This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board. Probable cause Suggested actions Ensure all fittings are tight Loose fitting Exchange defective tubing Broken tubing 1290 Infinity Flexible Cube User Manual...
  • Page 117 Blocked needle seat Configure tubing to position 4 of injection Tubing not correctly installed valve. Configure Flexible Cube correctly Flexible Cube not configured in software Please contact your Agilent service Defective flush pump representative 1290 Infinity Flexible Cube User Manual...
  • Page 118 • Check valve head for correct installation Defect Sensor on the Valve Drive Motor • Try to identify the source of trouble by installing a different valve head if possible. • Contact your Agilent Service representative. 1290 Infinity Flexible Cube User Manual...
  • Page 119 Soft power-down power supply of the valve drive. N O T E Whenever you want to power cycle the valve drive for a re-boot, it needs to be powered off for at least 10 seconds. 1290 Infinity Flexible Cube User Manual...
  • Page 120 Position Cluster Partner Missing Probable cause Suggested actions Check the CAN cable connections of the Communication issues modules. Check and correct if necessary the valve Configuration mismatch configuration and presence of defined position cluster partner. 1290 Infinity Flexible Cube User Manual...
  • Page 121 1290 Infinity Flexible Cube User Manual Test Functions Introduction Pressure Test This chapter describes the tests for the module. Agilent Technologies...
  • Page 122 Pressure Test For valves In Agilent Instrument Utilities, Agilent ChemStation, and Agilent Instant Pilot no tests are available for the Flexible Cube. For details on the use of the interface refer to the interface documentation. 1290 Infinity Flexible Cube User Manual...
  • Page 123 Do not use the test for modules having a lower maximum pressure than the pump as this will damage the valve. For example do not use 400 bar valve in a TCC or Flexible Cube in combination with a 600 bar pump. 1290 Infinity Flexible Cube User Manual...
  • Page 124 Test Functions Pressure Test 1290 Infinity Flexible Cube User Manual...
  • Page 125 1290 Infinity Flexible Cube User Manual Maintenance and Repair Introduction to Maintenance Warnings and Cautions Overview of Maintenance Cleaning the Module Exchange Flush Pump Inlet Valve Exchange Flush Pump Outlet Valve Exchange Valve Rotor Seal Replacing Parts of the Valve Head...
  • Page 126 Figure 27 on page 126 shows the main user accessible assemblies of the Agilent 1290 Infinity Flexible Cube. These parts can be accessed from the front (simple repairs) and don’t require to remove the Flexible Cube from the system stack.
  • Page 127 C AU T I O N ➔ If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment. 1290 Infinity Flexible Cube User Manual...
  • Page 128 If the valve performance page 139 shows indication of leakage or wear “Replacing Module If required Firmware” on page 150 Preventive maintenance is usually not necessary; only for the rotor seal! N O T E 1290 Infinity Flexible Cube User Manual...
  • Page 129 WA R N I N G hazard and damage the module ➔ Do not use an excessively damp cloth during cleaning. ➔ Drain all solvent lines before opening any connections in the flow path. 1290 Infinity Flexible Cube User Manual...
  • Page 130 • Open the front door of the module. Disconnect the finger tight fitting of the Inlet capillary at With a 14 mm wrench on the flange closest to the pump the bottom of the Flush pump. head, unscrew the valve. 1290 Infinity Flexible Cube User Manual...
  • Page 131 Reconnect the finger tight fitting at the inlet of the flush flange closest to the pump head. pump. Close the front cover and prime the flush pump before use to warrant trouble free usage. 1290 Infinity Flexible Cube User Manual...
  • Page 132 • Open the front door of the module. With a ¼ inch wrench disconnect the capillary at the top With a 14 mm wrench on the flange closest to the pump of the Outlet valve. head, unscrew the valve. 1290 Infinity Flexible Cube User Manual...
  • Page 133 Reconnect the capillary with a ¼ inch wrench at the flange closest to the pump head. outlet of the flush pump. Close the front cover and prime the flush pump before use to warrant trouble free usage. 1290 Infinity Flexible Cube User Manual...
  • Page 134 • In order to avoid leaks, place the solvent bottles at or below the level of the pump head. • Open the front door of the module. Remove all capillaries from the valve head. With a 9/64 inch hex key unscrew the stator screws. 1290 Infinity Flexible Cube User Manual...
  • Page 135 Maintenance and Repair Exchange Valve Rotor Seal Remove the stator head and the stator ring. Remove the rotor seal. Remove the isolation seal. Replace the isolation seal. 1290 Infinity Flexible Cube User Manual...
  • Page 136 With a 9/64 inch hex key alternately tighten the stator Reinstall all capillaries to the valve head depending on screws. the type and usage of the valve. Close the front cover and prime the flush pump before use to warrant trouble free usage. 1290 Infinity Flexible Cube User Manual...
  • Page 137 When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice. 1290 Infinity Flexible Cube User Manual...
  • Page 138 Always mind the correct orientation of the parts and avoid to touch their surfaces. N O T E 5 Turn each of the screws an equal amount until they are finger-tight, then tighten them for another half turn. 1290 Infinity Flexible Cube User Manual...
  • Page 139 5067-4273 6-column selector valve head, 1300 bar 5067-4148 2 position/6 port valve head, 600 bar, bio-inert 5067-4134 4-column selector valve head, 600 bar, bio-inert 5067-4159 12 position/13 port selector valve head, 210 bar, bio-inert 1290 Infinity Flexible Cube User Manual...
  • Page 140 Always install a valve head for operation and storage. For protecting the actuator, a dummy valve head (part of Transportation Lock Kit (G1316-67001 )) can be used instead of a functional valve. Do not touch parts inside the actuator. 1290 Infinity Flexible Cube User Manual...
  • Page 141 To have the valve correctly recognized by the Agilent Infinity Valve Drive you must have the N O T E valve drive powered off for at least 10 seconds. Switch off the module. Unscrew the valve head. Remove all capillary connections from the valve head 1290 Infinity Flexible Cube User Manual...
  • Page 142 “Installing the Valve union nut (see also “Installing the Valve Head and Heads” on page 53). Connecting Capillaries” on page 54. Install all required capillary connections to the valve Switch on the module. head. 1290 Infinity Flexible Cube User Manual...
  • Page 143 (0.12 mm i.d. or 0.17 mm i.d. depending on the capillary kit) (5067-4652) and the ports 9 and 10 need to be plugged with Plastic fittings (0100-1259). Figure 28 Re-routing of 2 position/10 port valve to match 2 position/6 port valve 1290 Infinity Flexible Cube User Manual...
  • Page 144 5067-4653 Capillary ST 0.12 mm x 200 mm S/SX Preparations Identify the required capillaries for your set up Use outmost care to avoid any void volumes caused by poor connections. N O T E 1290 Infinity Flexible Cube User Manual...
  • Page 145 Flow direction from top to bottom needs switch of connected capillaries at ports 5 and 2. Also column inlet connections needed to be switched with outlet connections. Port 4 to 3 and 6 to 1.) 1290 Infinity Flexible Cube User Manual...
  • Page 146 90 mm capillary to column Pos.1: Connection between ports 1-6 , 4-5, 2-3 , active column 1 = left (enrichment column) Pos. 2: Connection between ports 1-2, 3-4, 5-6 active column 2 = right (analytical colum) 1290 Infinity Flexible Cube User Manual...
  • Page 147 90 mm capillary to analytical column Pos. 1: Connection between ports 1-6, 4-5, 2-3, active column 1 = left (enrichment column) Pos. 2: Connection between ports 1-2, 3-4, 5-6, active column 2 = right (analytical colum) 1290 Infinity Flexible Cube User Manual...
  • Page 148 Maintenance and Repair Installing the Capillaries Figure 32 Installing the capillaries for alternating column regeneration (column at the second position omitted) 1290 Infinity Flexible Cube User Manual...
  • Page 149 7 Place the capillaries that go to another module or waste into the capillary guides to prevent squeezing them when closing the front cover. 8 Stow any excess lengths of the capillaries. 9 Perform a final leak-check. 1290 Infinity Flexible Cube User Manual...
  • Page 150 Update Tool and the documentation from the Agilent web. • http://www.agilent.com/en-us/firmwareDownload?whid=69761 2 For loading the firmware into the module follow the instructions in the documentation. Module Specific Information There is no specific information for this module. 1290 Infinity Flexible Cube User Manual...
  • Page 151 1290 Infinity Flexible Cube User Manual Parts and Materials for Maintenance Parts overview Capillaries Accessory Kits Valve Options Overview 2 Position/6 Port Valve Head 800 bar, 5067-4282 2 Position/6 Port Valve Head 1300 bar, 5067-4241 2 Position/6 Port Valve Head 600 bar (Bio-inert), 5067-4148...
  • Page 152 Parts overview Item Description 5067-4717 Inlet valve 5067-4716 Outlet valve G4280-67304 Solvent selection valve to flush pump tubing 5067-4680 Tubing Kit 600 mm; 130 bar 5067-4697 Solvent selection valve bridge tubing Figure 33 Parts overview 1290 Infinity Flexible Cube User Manual...
  • Page 153 Parts and Materials for Maintenance Capillaries Capillaries Item Description 5067-4680 Tubing Kit 600 mm; 130 bar G4280-67304 Solvent selection valve to flush pump tubing 5067-4697 Solvent selection valve bridge tubing 1290 Infinity Flexible Cube User Manual...
  • Page 154 Parts and Materials for Maintenance Accessory Kits Accessory Kits Tool Kit G7120-68708 1290 Infinity Flexible Cube User Manual...
  • Page 155 5067-4680 Tubing Kit 600 mm; 130 bar 5181-1519 CAN cable, Agilent module to module, 1 m 9301-6524 Solvent bottle, clear, 1000 mL G4220-60007 Bottle Head Assembly G4226-87012 Needle seat 5043-0909 Tubing-Flex PE, 3 m 1290 Infinity Flexible Cube User Manual...
  • Page 156 Valve kits include the valve head, optional capillary kits, manual, access material and installation and familiarization service. For more details refer to the 'Parts and Material' section. G4230A includes 2 x 8 position/9 port valve head, 600bar. 1290 Infinity Flexible Cube User Manual...
  • Page 157 210 bar, bio-inert face kit, bio-inert G5639A 5067-4134 5068-0045 5068-0044 4-column selector 4-column selector, rotor seal, bio-inert stator head, bio-inert valve head, bio-inert 600 bar, bio-inert kit with stator face and rotor seal 1290 Infinity Flexible Cube User Manual...
  • Page 158 2 Position/6 Port Valve Head 800 bar, 5067-4282 2 Position/6 Port Valve Head 800 bar, 5067-4282 Item Description 1535-4857 Stator screws, 10/Pk 0101-1417 Stator head 0101-1409 Rotor Seal, PEEK 1535-4045 Bearing ring Figure 34 Column Switching Valve Parts (5067-4282) 1290 Infinity Flexible Cube User Manual...
  • Page 159 2 Position/6 Port Valve Head 1300 bar, 5067-4241 2 Position/6 Port Valve Head 1300 bar, 5067-4241 Item Description 1535-4857 Stator screws 5068-0006 Stator head 5068-0207 Rotor Seal (PEEK) 1535-4045 Bearing ring Figure 35 Column Switching Valve Parts (5067-4241) 1290 Infinity Flexible Cube User Manual...
  • Page 160 For bio-inert modules use bio-inert parts only! Item Description 5068-0020 Stator Screws, 10/pack 5068-0060 Bio-inert stator head 0100-1851 Stator face, ceramic 0101-1409 Rotor Seal, PEEK 1535-4045 Bearing ring Figure 36 Column Switching Valve Parts (5067-4148) 1290 Infinity Flexible Cube User Manual...
  • Page 161 2 Position/10 Port Micro Valve Head 600 bar, 5067-4144 2 Position/10 Port Micro Valve Head 600 bar, 5067-4144 Item Description 5068-0054 Stator screws, 10/Pk 0101-1421 Stator Head 0101-1415 Rotor Seal, PEEK 1535-4045 Bearing ring Figure 37 Column Switching Valve Parts (5067-4144) 1290 Infinity Flexible Cube User Manual...
  • Page 162 2 Position/10 Port Valve Head 1300 bar, 5067-4240 2 Position/10 Port Valve Head 1300 bar, 5067-4240 Item Description 5068-0019 Stator screws 5068-0011 Stator head 5068-0205 Rotor Seal (PEEK) 1535-4045 Bearing ring Figure 38 Column Switching Valve Parts (5067-4240) 1290 Infinity Flexible Cube User Manual...
  • Page 163 8 Position/9 Port Valve Head 600 bar, 5067-4107 8 Position/9 Port Valve Head 600 bar, 5067-4107 Item Description 1535-4857 Stator screws 5068-0001 Stator head 5067-4111 Rotor Seal, PEEK 1535-4045 Bearing ring Figure 39 Column Switching Valve Parts (5067-4107) 1290 Infinity Flexible Cube User Manual...
  • Page 164 For bio-inert modules use bio-inert parts only! Item Description 5068-0059 Stator screws 5068-0044 Bio-inert stator head 5068-0093 Stator face assy 5068-0045 Bio-inert rotor seal, PEEK 1535-4045 Bearing ring Figure 40 Column Switching Valve Parts (5067-4134) 1290 Infinity Flexible Cube User Manual...
  • Page 165 6-Column Selector Valve Head 800 bar, 5067-4284 6-Column Selector Valve Head 800 bar, 5067-4284 Item Description 5068-0089 Stator screws 5068-0241 Stator Head 5068-0298 Rotor Seal (PEEK) 1535-4045 Bearing ring Figure 41 Column Switching Valve Parts (5067-4284) 1290 Infinity Flexible Cube User Manual...
  • Page 166 6-Column Selector Valve Head 1300 bar, 5067-4273 6-Column Selector Valve Head 1300 bar, 5067-4273 Item Description 5068-0089 Stator screws 5068-0241 Stator Head 5068-0242 Rotor Seal (PEEK) 1534-4045 Bearing ring Figure 42 Column Switching Valve Parts (5067-4273) 1290 Infinity Flexible Cube User Manual...
  • Page 167 For bio-inert modules use bio-inert parts only! Item Description 5068-0059 Stator screws 5068-0097 Bio-inert stator head 0101-1288 Bio-inert rotor seal and stator face seal, kit 1535-4045 Bearing ring Figure 43 Column Switching Valve Parts (5067-4159) 1290 Infinity Flexible Cube User Manual...
  • Page 168 Parts and Materials for Maintenance 12 Position/13 Port Valve Head 210 bar (Bio-inert), 5067-4159 1290 Infinity Flexible Cube User Manual...
  • Page 169 1290 Infinity Flexible Cube User Manual Identifying Cables Cable Overview Analog Cables Remote Cables BCD Cables CAN/LAN Cables External Contact Cable Agilent Module to PC Agilent 1200 Module to Printer This chapter provides information on cables used with the 1290 series of HPLC modules.
  • Page 170 Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent module to 3394/6 integrators...
  • Page 171 It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m 1290 Infinity Flexible Cube User Manual...
  • Page 172 The other end depends on the instrument to which connection is being made. Agilent Module to 3394/6 Integrators p/n 35900-60750 Pin 3394/6 Pin Agilent Signal Name module Not connected Shield Analog - Center Analog + 1290 Infinity Flexible Cube User Manual...
  • Page 173 Pin BNC Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog + 1290 Infinity Flexible Cube User Manual...
  • Page 174 Identifying Cables Remote Cables Remote Cables One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Agilent Module to 3396A Integrators...
  • Page 175 3 - Gray Start 4 - Blue Shut down 5 - Pink connected 6 - Yellow Power on High 7 - Red Ready High 8 - Green Stop 9 - Black Start request 13, 15 connected 1290 Infinity Flexible Cube User Manual...
  • Page 176 01046-60201 Wire Color Pin Agilent Signal Name Active module (TTL) White Digital ground Brown Prepare run Gray Start Blue Shut down Pink connected Yellow Power on High Ready High Green Stop Black Start request 1290 Infinity Flexible Cube User Manual...
  • Page 177 Black BCD 0 Orange BCD 3 BCD 2 Brown BCD 1 Gray Digital ground Gray Gray/pink BCD 11 Red/blue BCD 10 White/green BCD 9 Brown/green BCD 8 not connected not connected + 5 V 1290 Infinity Flexible Cube User Manual...
  • Page 178 Agilent Module to 3396 Integrators p/n 03396-60560 Pin 3396 Pin Agilent Signal Name BCD Digit module BCD 5 BCD 7 BCD 6 BCD 4 BCD0 BCD 3 BCD 2 BCD 1 Digital ground + 5 V 1290 Infinity Flexible Cube User Manual...
  • Page 179 CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) 1290 Infinity Flexible Cube User Manual...
  • Page 180 EXT 2 Grey EXT 3 Pink EXT 3 Blue EXT 4 EXT 4 Black Not connected Violet Not connected Grey/pink Not connected Red/blue Not connected White/green Not connected Brown/green Not connected White/yellow Not connected 1290 Infinity Flexible Cube User Manual...
  • Page 181 It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m 1290 Infinity Flexible Cube User Manual...
  • Page 182 Agilent 1200 Module to Printer Description 5181-1529 Cable Printer Serial & Parallel, is a SUB-D 9 pin female vs. Centronics connector on the other end (NOT FOR FW UPDATE). For use with G1323 Control Module. 1290 Infinity Flexible Cube User Manual...
  • Page 183 1290 Infinity Flexible Cube User Manual Hardware Information Firmware Description Electrical Connections Rear view of the module Interfaces Overview Interfaces Setting the 8-bit Configuration Switch (without On-board) LAN Communication Settings for RS-232C Special Settings Instrument Layout Early Maintenance Feedback This chapter describes the module in more detail on hardware and electronics.
  • Page 184 • run synchronization through APG remote, • error handling, • diagnostic functions, • or module specific functions like • internal events such as lamp control, filter movements, • raw data collection and conversion to absorbance. 1290 Infinity Flexible Cube User Manual...
  • Page 185 Update of main system can be done in the resident system only. Update of the resident N O T E system can be done in the main system only. Main and resident firmware must be from the same set. Figure 44 Firmware Update Mechanism 1290 Infinity Flexible Cube User Manual...
  • Page 186 All this specific information is described in the documentation provided with the firmware update tools. The firmware update tools, firmware and documentation are available from the Agilent web. • http://www.agilent.com/en-us/firmwareDownload?whid=69761 1290 Infinity Flexible Cube User Manual...
  • Page 187 There are no externally accessible fuses, because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.
  • Page 188 Hardware Information Electrical Connections Rear view of the module Figure 45 Rear view of the Flexible Cube 1290 Infinity Flexible Cube User Manual...
  • Page 189 G1364C FC-AS G1330B/K1330B μ CAN-DC- OUT for CAN G1364D FC- slaves G1367E HiP ALS K1367E HiP ALS Clinical Ed. G1377A HiP micro ALS G2258A DL ALS G5664A Bio-inert FC-AS G5667A Bio-inert Autosampler G4226A ALS 1290 Infinity Flexible Cube User Manual...
  • Page 190 K1321B FLD Clinical Ed. G1321C FLD G1362A RID G4280A ELSD EXT Contact AUTOZERO Others G1170A Valve Drive G1316A/C TCC K1316C TCC Clinical Ed. G1322A DEG K1322A DEG Clinical Ed. G1379B DEG G4225A DEG K4225A DEG Clinical Ed. 1290 Infinity Flexible Cube User Manual...
  • Page 191 • CAN connectors as interface to other modules • LAN connector as interface to the control software • RS-232C as interface to a computer • REMOTE connector as interface to other Agilent products • Analog output connector(s) for signal output 1290 Infinity Flexible Cube User Manual...
  • Page 192 There is no configuration possible on main boards with on-board LAN. These are N O T E pre-configured for • 19200 baud, • 8 data bit with no parity and • one start bit and one stop bit are always used (not selectable). 1290 Infinity Flexible Cube User Manual...
  • Page 193 RS-232C Connection Table Direction Function Ground Figure 46 RS-232 Cable Analog Signal Output The analog signal output can be distributed to a recording device. For details refer to the description of the module’s main board. 1290 Infinity Flexible Cube User Manual...
  • Page 194 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
  • Page 195 START REQUEST (L) Request to start injection cycle (for example, by start key on any module). Receiver is the autosampler. Special Interfaces There is no special interface for this module. 1290 Infinity Flexible Cube User Manual...
  • Page 196 • for boot/test modes DIPS 1+2 must be UP plus required mode For normal operation use the default (best) settings. N O T E Switch settings provide configuration parameters for serial communication protocol and instrument specific initialization procedures. 1290 Infinity Flexible Cube User Manual...
  • Page 197 Once the change has been completed, the module must be powered up again in order to store the values in the non-volatile memory. Table 14 Communication Settings for RS-232C Communication (without on-board LAN) Mode Select RS-232C Baudrate Data Bits Parity 1290 Infinity Flexible Cube User Manual...
  • Page 198 Parity No Parity Odd Parity Even Parity One start bit and one stop bit are always used (not selectable). Per default, the module will turn into 19200 baud, 8 data bit with no parity. 1290 Infinity Flexible Cube User Manual...
  • Page 199 Save your methods and data before executing a forced cold start. If you use the following switch settings and power the instrument up again, a forced cold start has been completed. Table 19 Forced Cold Start Settings (without on-board LAN) Mode Select TEST/BOOT 1290 Infinity Flexible Cube User Manual...
  • Page 200 • the plastic layers help cushion the electronic and mechanical parts from physical shock, and • the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself. 1290 Infinity Flexible Cube User Manual...
  • Page 201 EMF limits, and then reset the EMF counters to zero. The next time the EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. 1290 Infinity Flexible Cube User Manual...
  • Page 202 Hardware Information Early Maintenance Feedback 1290 Infinity Flexible Cube User Manual...
  • Page 203 1290 Infinity Flexible Cube User Manual Appendix General Safety Information The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Radio Interference Sound Emission Agilent Technologies on Internet This chapter provides addition information on safety, legal and web. Agilent Technologies...
  • Page 204 C AU T I O N alerts you to situations that could cause loss of data, or damage of equipment. ➔ Do not proceed beyond a caution until you have fully understood and met the indicated conditions. 1290 Infinity Flexible Cube User Manual...
  • Page 205 Make sure that only fuses with the required rated current and of the specified type (normal blow, time delay, and so on) are used for replacement. The use of repaired fuses and the short-circuiting of fuse holders must be avoided. 1290 Infinity Flexible Cube User Manual...
  • Page 206 When working with solvents, observe appropriate safety procedures (for example, goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet by the solvent vendor, especially when toxic or hazardous solvents are used. 1290 Infinity Flexible Cube User Manual...
  • Page 207 The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste. Do not dispose of in domestic household waste N O T E To return unwanted products, contact your local Agilent office, or see http://www.agilent.com for more information. 1290 Infinity Flexible Cube User Manual...
  • Page 208 Appendix Radio Interference Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with equipment unscreened...
  • Page 209 This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) 1290 Infinity Flexible Cube User Manual...
  • Page 210 Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com 1290 Infinity Flexible Cube User Manual...
  • Page 211 Flexible Cube valve switching failed cable one stack external contact bench space control settings cable bio-inert defect on arrival fan failed cable delivery checklist features analog dimensions firmware description main system 1290 Infinity Flexible Cube User Manual...
  • Page 212 1290 Infinity Flexible Cube User Manual...
  • Page 213 102, status indicators 102, unpacking user interfaces user interface flexible cube valve dummy remove valve heads replace valve head install valve rotor seal exchange voltage range 1290 Infinity Flexible Cube User Manual...
  • Page 214 • introduction and specifications, • installation, • using and optimizing, • troubleshooting and diagnose, • maintenance, • parts identification, • hardware information, • safety and related information. © Agilent Technologies 2010-2018 Printed in Germany 10/2018 *G4227-90000* *G4227-90000* G4227-90000 Rev. C Agilent Technologies...

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