Priming The Pump - Agilent Technologies 1290 Infinity LC System System Manual And Quick Reference

Priming the Pump

This procedure is required when...
• the pump is used for the first time
• whenever one or more of the inlet tubes contains air gaps or is dry for other
The purpose of priming is to remove all air bubbles from the pump and its inlet
tubes.
Propan-2-ol (alternative names: isopropanol, isopropyl alcohol, IPA) is the best
solvent for priming the pump from dry and has the advantage of being
miscible with most normal phase or reverse phase solvents (check a solvent
miscibility table if unsure).
Toxic, flammable and hazardous solvents, samples and reagents
WA R N I N G
The handling of solvents, samples and reagents can hold health and safety risks.
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➔
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Avoid running the solvent reservoirs to emptiness by using the Bottle Filling monitor. Use a
N O T E
syringe to fill the solvent tubes, the pump will not pump air.
Before starting the procedure ensure that all the solvent lines are connected to the pump
N O T E
as detailed in the pump installation manual, and that the waste tubes are safely positioned
in a suitable waste solvent container. Ensure that the pump is controlled by computer
software or the Instant Pilot controller and that the flow rate is set to zero.
Agilent 1290 Infinity LC System Manual and Quick Reference
reasons
When working with these substances observe appropriate safety procedures (for
example by wearing goggles, safety gloves and protective clothing) as described in
the material handling and safety data sheet supplied by the vendor, and follow good
laboratory practice.
The volume of substances should be reduced to the minimum required for the
analysis.
Do not operate the instrument in an explosive atmosphere.
System Setup and Installation
Installing the Module
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