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3
Optimization of the Agilent 1290 Infinity LC System
How to Configure the Optimum Delay Volume
How to Configure the Optimum Delay Volume
Table 3
contribute to system delay volume in the Agilent 1290 Infinity LC System. In
the standard configuration with the Agilent 1290 Infinity Binary Pump, with
the Jet Weaver mixer, the 1290 Infinity Autosampler and Thermostatted
Column Compartment the system delay volume is about 125 µl. This standard
delay volume is suitable for the majority of applications. For example, a fast
separation on a 50 mm x 2.1 mm column packed with sub-two-micron
particles at a moderate flow rate of 0.6 ml/min will result in a typical gradient
delay time of about 0.2 min which is generally acceptable with gradient times
of two to three minutes (see
the flow rate in terms of column volumes and it can be seen from
page 44 that with this column at 0.6 ml/min about 6 column volumes per
minute flow through the system and the delay volume is about 1.2 times the
column volume.
A setup with the Agilent 1290 Infinity Quaternary Pump, the 1290 Infinity
Autosampler, and Thermostatted Column Compartment has a delay volume of
430 µL, resulting in a delay time of 0.7 min. This is the limit for acceptance of
gradient times of 3 min.
Table 3
Components
Binary Pump
Jet Weaver mixer (standard)
Binary Pump + Jet Weaver
Quaternary Pump
Quaternary Pump + V380 Jet Weaver
Autosampler (fixed 5 µl loop)
Autosampler (standard, variable volume)
Column Compartment low-dispersion heat exchanger
Connection tubing, 0.12 mm i.d., per 100 mm
42
on page 42 and
Table 4
Delay volumes of 1290 Infinity LC modules
Agilent 1290 Infinity LC System Manual and Quick Reference
on page 43 show the component volumes which
Table 6
on page 43). It is often useful to consider
Table 7
Delay Volume (µl)
10
35
45
350
500
5
80
1.6
1.1
on
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