Agilent Technologies 1290 Infinity LC System System Manual And Quick Reference page 69

9 Purge the pumps (the connections up to the column) of any buffer
10 Flush the column with compatible mobile phase starting slowly at
11 Once the pressure has stabilized, attach the column to the detector.
12 Equilibrate the column and detector with 10 column volumes of the mobile
13 Avoid over-pressure. Check the pressure range of your gradient before
14 If samples are dissolved in high organic content consider using an injection
Agilent 1290 Infinity LC System Manual and Quick Reference
containing mobile phases and flush through 5 ml of solvent before attaching
the column to the instrument.
0.1 ml/min for a 2.1 mm inner diameter column, 0.2 ml/min for a 3.0 mm
inner diameter column, and 0.4 ml/min for 4.6 mm inner diameter. Increase
the flow rate to the desired flow over 5 minutes.
phase prior to use (see
starting any sequence.
program to add a plug of weaker solvent before and after the sample aliquot
to reduce the risk of precipitation in high concentration buffer mobile
phase.
Optimization of the Agilent 1290 Infinity LC System
How to Prevent Column Blockages
Table 7 on page 44 for column volumes).
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