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C.3.5 Supernatant Collection
Example: Aspirate 100 µl from 8 rows of a Microtiter
total volume.
s
Vol
[Fill]
(100) [Enter]
t
[Fill] [Enter]
[Fill] [Enter]
[Fill] [Enter]
[Fill] [Enter]
[Fill] [Enter]
[Fill] [Enter]
[Fill] [Enter]
[Purge] [Enter] [Enter]
C.4 Operating Procedures
After programming the pipette, the display should show the first program step,
with the "Run" prompt showing in the lower left corner:
The following steps describe a basic program for pipetting reagent into a
Microtiter plate. The pipette is programmed as follows:
[Fill]
[Disp]
[Disp]
Figure C.4: Run Prompt
s
Vol
(250) [Enter]
t
s
Vol
(10) [Enter]
t
s
Vol
(30) [Enter] [Enter]
t
This program is performed with the 1250 µl
Equalizer pipette. All 100 µl fill volumes are
collected in the same tips. When completed, the
total volume is discarded with the purge step.
Programming and Operation
plate and discard the
®
Note: The 10 µl Primer
Dispense reduces the
percentage of error in
incremental dispensing
(see section D.3
Recommendations for
Accurate Pipetting)
21
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