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2. Select pipetting operation. Enter the programming mode by pressing the
[Fill] or [Mix] key.
For example, to begin
programming with a
filling operation, press
the [Fill] key. The display
shows "Fill" with a
flashing "Vol" prompt:
Note: The displayed volume defaults to the last volume programmed for
the selected operation, or to the default volume for the pipette, if it is being
programmed for the first time.
Fill. The [Fill] key programs the pipette to aspirate a volume of liquid. A
single fill step can be programmed for fluid transfers, or a combination of
fill steps can be programmed for dilutions or supernatant collection.
Dispense. The [Disp] key programs the pipette to dispense a volume
of liquid. The dispense volume can be the entire fill volume or a smaller
increment for repeat pipetting. The dispense volume needs to be
programmed only once for repeat pipetting. The pipette will dispense the
volume each time the trigger is pressed (or repeatedly during a paced
dispense) until the fill volume is reached, or until insufficient volume
remains.
Mix. The [Mix] key programs the pipette to perform a combination of fills
and dispenses. You can program a mix volume or mix cycle (single fill and
dispense). You can press [Mix] once to program a mix volume, or twice to
program a mix cycle. Mix cycles from 2 to 9 are available on the Equalizer
pipette. The default setting is three mix cycles.
Some programming considerations for the mix operation:
If the program begins a mix cycle from zero position, it will complete the
cycle with an automatic blowout, then display "ZERO." Press the trigger to
return the pistons to zero position.
Programming and Operation
Figure C.2.2a: Flashing Volume Display
15
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