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Programming and Operation
In this program, only one dispense step needs to be programmed. If any
program ends in a dispense step, the pipette repeats the dispense step
until the volume is zero, or is less than the programmed dispense volume.
If the remaining volume is less than the programmed dispense volume, the
pipette displays a "Purge" prompt to discard the remainder. This discarded
volume contains the cumulative error of previous pipettings (see section D.3
Recommendations for Accurate Pipetting for further explanation).
C.3.3 Serial Dilutions
Example: Transfer 100 µl to the first column of a plate previously filled with
reagent. Mix 200 µl for 2 cycles. Transfer 100 µl to the second column and
repeat the mix step. Follow this procedure for the rest of the plate.
[Fill]
[Mix]
That's the entire program! The mix step always returns to the volume
at which it started (100 µl); therefore, the pipette continues the mix step
indefinitely, until you press the [Purge] key to end the program. In this
example, the mix cycle is programmed for 2 cycles (default is 3 cycles).
C.3.4 Simple Dilutions
Example: Prepare 1:10 dilution with a 10 µl sample volume.
The first fill volume is for the diluent (100 µl), followed by an air gap (20 µl),
and finally by the sample volume (10 µl). The purge step will dispense the
total volume.
20
s
Vol
(100) [Enter]
t
s
Vol
(200) [Mix]
t
s
Vol
[Fill]
t
s
Vol
[Fill]
t
s
Vol
[Fill]
t
[Purge] [Enter] [Enter]
s
Vol
(2) [Enter] [Enter] [Enter]
t
(100) [Enter]
(20) [Enter]
(10) [Enter]
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