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Programming and Operation
1. Attach pipette tips. When ready to begin pipetting, attach the tips to
the pipette.
Note: When applying tips, avoid 'banging' or applying excessive force to the
tip(s), as this stretches the tip seal and will affect volume. Use Matrix tips which
have been specifically designed to match the tip fittings on Matrix pipettes.
2. Aspirate liquid. Immerse the pipette tips into the liquid to be aspirated,
then press the trigger to aspirate 250 µl. Submerge the tips about 1/4"
below the surface of the liquid.
3. Dispense primer volume. Discard the 10 µl primer volume back to the
source vessel or into a waste receptacle.
4. Dispense liquid. Position the pipette tips over the first column of wells
in the Microtiter plate, and press the trigger to dispense 30 µl. At the
completion of the dispense step, the pipette emits a single beep tone (if the
beep tone is set to ON1).
5. Repeat dispense. Repeat the previous step for the rest of the columns in
the Microtiter plate. At the completion of the eighth dispense, the pipette
emits a double beep tone (if the beep tone is on, otherwise it emits a single
beep tone) and displays the "Purge" prompt. There is insufficient volume
for another 30 µl dispense, and the pipette signals for a purge step.
-or-
Paced Dispense. Steps 4 and 5 can also be performed as a paced
dispense. Instead of pressing the trigger for each dispense step, press and
hold the trigger to automatically dispense each volume. Immediately after
each dispense step, the pipette emits a single beep tone (if the beep tone is
on), prompting you to prepare for the next dispense step. To stop a paced
dispense, release the trigger before the end of the last dispense step.
Note: For paced dispensing of small volumes, set the pace to SLOW. Ensure
that you have fully released the trigger before the end of the last dispense step
to prevent an additional, unwanted dispense step.
6. Discard remaining liquid. Position the pipette tips over a waste container
and press the trigger to purge the remaining liquid. The pipette emits a
triple beep tone to signal the end of the program, then returns to the first
step of the program.
7. Eject tips. Press the ejector trigger to eject the used pipette tips.
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