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F.1.2 Equalizer Pipette Cleaning and Autoclaving
1. Remove the piston assembly
from the cylinder.
2. Remove and discard the cross
tubes into a biohazardous
container.
3. Remove the O-rings (only if
necessary, see section F.1.3
for O-ring Replacement)
and discard them into a
biohazardous container.
4. Place all components (see Figure F.1.2) into the autoclave and autoclave at
121 °C for 20 minutes.
F.1.3 Equalizer Pipette Maintenance and Reassembly
Once components are autoclaved and completely dry, use the following
procedures to relubricate and reassemble the unit:
O-ring Replacement
The O-rings may require replacement if liquids have been over aspirated. In
this case, the O-rings should be removed prior to autoclaving. Spare O-ring
packs are available from Thermo Fisher Scientific for all pipette volumes.
1. Remove old O-rings.
• 1250 μl, 250 μl Pipettes: O-rings can be removed from the pistons by
squeezing one side of the O-ring and lifting it out of the O-ring groove
on the piston. Once removed, the O-rings should be discarded as
biohazardous waste.
• 125 μl, 30 μl, 12.5 μl Pipettes: O-rings from these pipettes are located in
the cylinder. To remove the O-rings, unscrew the four screws that hold
the cylinder cap to the cylinder (see Figure F.2.3a). Once removed, the
O-rings should be discarded as biohazardous waste.
Figure F.1.2: Components to be Autoclaved
Maintenance
35
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