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Calibration
D.2 Pipette Calibration
Equalizer pipette users can calibrate their pipettes with "EASY" CAL mode
for distilled water or with "CALC" mode to calibrate other liquids and for
temperatures other than room temperature.
D.2.1 Factory Calibration Step
The factory calibration step is needed when calibrating your Equalizer pipette.
This is located on the calibration certificate called "Pipette Calibration Report"
enclosed in the documention for this pipette. (See comments on page 1 of
Pipette Calibration Report). View the pipette's current calibration step by using
the CALVIEW mode listed in section D.2.4.
Note: When resetting the calibration step to a default value, as listed in section
D.2.5 CALRESET, the step is set to an average value for all pipettes of its class.
This value may differ from the factory-optimized calibration step documented
on the "Pipette Calibration Report". Please use the step listed there for best
results. If you cannot locate you documentation that lists the factory calibration
step, contact Thermo Fisher Scientific Tech Support at 800-345-0206, and have
your pipette's serial number ready.
D.2.2 "EASY" Cal Mode
Equalizer pipette users can quickly calibrate their pipette by using the unique
"EASY" CAL feature. "EASY" CAL has been developed for fast, easy, in-lab
verification and/or calibration using distilled water at room temperature
(20-22.5 °C).
Volume
12.50 µl
30 µl
125.0 µl
250.0 µl
1250 µl
1. Weigh out ten aliquots of distilled water on an analytical balance, using
one pipetting channel. The programmed dispense volume must correspond
to the calibration point for that pipette (for example, 200 µl for a 250 µl
pipette). Refer to Table D.2.2, Calibration Specifications, for proper
calibration points.
24
Calibration Point (µl)
10.00 µl
25.0 µl
100 µl
200 µl
1000 µl
Table D.2.2: Calibration Specifications
Factory Calibration Steps
Located on the calibration
certificate "Pipette Calibration
Report" sheet included in the
pipette documentation.
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