Table of Contents
Advertisement
2 Hardware Installation (Differences)
Preparing to Condition the Column
9 Verify column flow by placing the outlet end of the column in a beaker of
water and checking for bubbles at the column outlet.
Allow five minutes before applying heat to any GC thermal zone. This wait time is
to purge trapped air from the column.
10 In the Status/Settings/Inlet screen, set the Inlet to 300° C.
11 In the Status/Settings/Oven screen, set the initial oven temperature to
40° C.
12 Set the following oven temperature program in the Status/Settings/Oven
screen:
— 1 minute intial time
— 5° C/min ramp rate
— 280° C final temperature
— 71 minute hold in (at 280° C)
— 35° C post-run temperature
This procedure is for conditioning an HP5-MS capillary column. The temperatures
of the GC thermal zones (injection port, column oven and GC/MS interface) must
never exceed the upper limit specified for the column.
13 In the Status/Settings/Column screen, verify that the run time is 120
minutes.
14 In the Status /Settings/Detector screen, verify that the screen is titled
"Mass Selective Detector Transfer Line" and that the message "Heater
Missing" is displayed.
15 In the Status/Settings/Auxillary screen, verify that the No Auxiliary
option is selected from the Auxiliary Type field.
Return to the Status Screen.
16 In the Status screen, select Method Files.
17 Verify that the method parameters are correct (see Appendix C for a
complete list of PREP_RUN GC method parameters).
18 Select the Save (F8) key.
28
Advertisement
Table of Contents
