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Agilent G2545A Hybridization Oven
Calibration Procedure
Step 1. Set up and heat the oven
Step 2. Start the digital recording thermometer
Step 3. Retrieve the temperature data from the digital recording
thermometer
Step 4. Calibrate the oven
The Agilent G2545A Hybridization Oven maintains temperature calibration
for at least 3 months under normal operating conditions when properly
installed within the laboratory.
See the Agilent G2545A Hybridization Oven Installation, Operation, and
Maintenance Guide (p/n G2545-9001) for information on site
requirements and oven placement.
Calibrate the Agilent G2545A Hybridization Oven:
• After first installation in a working environment
• After each 3 months of use
• When systematic high microarray background noise may indicate a drift
in oven temperature
Do the calibration steps in this guide to maintain optimal performance for
your Agilent G2545A Hybridization Oven.
To view a demonstration of the hybridization oven
calibration procedure, go to
http://www.agilent.com/genomics/protocolvideos, or scan this
QR code with your mobile device.
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Summary of Contents for Agilent Technologies G2545A
- Page 1 The Agilent G2545A Hybridization Oven maintains temperature calibration for at least 3 months under normal operating conditions when properly installed within the laboratory. See the Agilent G2545A Hybridization Oven Installation, Operation, and Maintenance Guide (p/n G2545-9001) for information on site requirements and oven placement.
- Page 2 Required Parts and Tools Part Vendor and part/model hybridization oven Agilent p/n G2545A hybridization oven rotator rack Agilent p/n G2530-60029 hybridization chambers Agilent p/n G2534A Fourtec MicroLite II USB digital recording MicroDAQ.com thermometer or equivalent • p/n LITE5008P (8,000 readings) •...
- Page 3 Step 1. Set up and heat the oven Step 1. Set up and heat the oven 1 Install the hybridization oven rotator rack into the hybridization oven. 2 Load the hybridization oven rotator rack with the typical number of hybridization chambers used during a single hybridization run.
- Page 4 Step 2. Start the digital recording thermometer Step 2. Start the digital recording thermometer • Set up and start the digital recording thermometer. The software instructions are for the Fourtec MicroLite II USB digital recording thermometer. The hardware instructions are the same for all digital recording thermometers.
- Page 5 Step 2. Start the digital recording thermometer 6 Set Temperature Scale to °C. 7 Mark the Timer Run check box and select the date and time to start the recorder. 8 Set Interval to 00:05:00 (5 minutes). 9 Click Setup. 10 Click Yes to continue.
- Page 6 Step 2. Start the digital recording thermometer Make sure that the digital recording thermometer is secure and will not detach from CA UT IO N the rack. If the digital recording thermometer comes loose during use, the data collected can be inaccurate. A loose digital recording thermometer that becomes trapped and prevents the rotator rack from rotating can damage the oven.
- Page 7 Step 3. Retrieve the temperature data from the digital recording thermometer Step 3. Retrieve the temperature data from the digital recording thermometer • Follow the instructions for the digital recording thermometer to retrieve the temperature data. These steps are for the Fourtec MicroLite II USB digital recording thermometer.
- Page 8 Step 3. Retrieve the temperature data from the digital recording thermometer Figure 1 Temperature reading. Period of stable temperature is highlighted. Take the temperature reading from the period of stable temperature data recorded before the oven door is opened. Hybridization Oven Calibration Procedure...
- Page 9 Step 4. Calibrate the oven Step 4. Calibrate the oven 1 Compare the temperature reading from the digital recording thermometer with the temperature on the digital display on the control panel of the hybridization oven. Make sure that the oven temperature is stable after the digital recording thermometer is removed and before you adjust the...
- Page 10 Agilent Technologies, Inc. 2014 Revision A1, February 2014 *G2545-90002* G2545-90002...