Available Rotors - Beckman Coulter Airfuge Instructions For Use Manual

Air driven ultracentrifuge
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Available Rotors

Table 1.1 Specifications (Continued)
Specification
Installation (overvoltage) category
Pollution degree
a. Relative Centrifugal Field (RCF) is the ratio of the centrifugal acceleration at a specified radius and speed (rw2) to the
standard acceleration of gravity (g) according to the following formula: RCF = rw2/g where r is the radius in millimeters, w
is the angular velocity in radians per second (2 p RPM/60), and g is the standard acceleration of gravity (9807 mm/s2). After
substitution: RCF = 1.12 r (RPM/100)2.
b. Instrument operation at gauge pressures below 20 psig (138 kPa) may cause rotor instability.
c. Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation
must be expected.
Available Rotors
Airfuge rotors are made of anodized aluminum and have turbine flutes on the bottom that provide
the driving surface for the jets of air. Pressurized air impinges on the rotor bottom and lifts and
turns the rotor. A white plastic bushing fitted in the rotor bottom is engaged by the brake pin
during braking.
Rotors are designed to meet specific needs. Detailed information about each rotor is contained in
the applicable rotor manual.
• The A-110 fixed angle rotor holds up to six 180 L tubes at a tube angle of 18 degrees from the
axis of rotation, and is ideal for the rapid separation of small sample volumes in a very short
time. Maximum operating speed is 110 000 rpm at 30 psig (207 kPa) air pressure.
• The A-100/18 fixed angle rotor holds up to six 175 L tubes at a tube angle of 18 degrees from
the axis of rotation, and is ideal for the efficient sedimentation of small sample volumes in a
very short time. Maximum operating speed is 95 000 rpm at 30 psig (207 kPa) air pressure.
• The A-100/30 fixed angle rotor holds up to six 240 L tubes at a tube angle of 30 degrees from
the axis of rotation, and is ideal for the efficient sedimentation of small sample volumes in a
very short time. Maximum operating speed is 92 000 rpm at 30 psig (207 kPa) air pressure.
• The A-95 fixed angle rotor holds up to four 450 L tubes at a tube angle of 30 degrees from the
axis of rotation, and is ideal for the rapid separation of small sample volumes in a very short
time or to isolate microsomal fractions and plasma membranes. It can also be used to clarify
small volumes of lipemic serum by flotation of the chylomicrons. Maximum operating speed is
95 000 rpm at 30 psig (207 kPa) air pressure.
• The ACR-90 rotor uses 3.5 mL and 2.4 mL disposable liners, and is used to clarify lipemic serum.
Serum can be clarified by a 10 minute spin at 90 000 rpm at 30 psig (207 kPa) air pressure.
• The EM-90 electron microscopy particle-counting rotor is specifically designed for sedimenting
particulates from very small volumes onto supports suitable for insertion into an electron
microscope. The rotor is rated for 90 000 rpm at 30 psig (207 kPa) air pressure.
• The Batch rotor can be used to pellet particles from a single large sample. Sample volumes of
2 to 7 mL can be run. The rotor is rated for 90 000 rpm at 30 psig (207 kPa) air pressure.
1-8
Description
II
c
2
AF-IM-13AC

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