Characterizing The Needle And Sample Loop Volumes - Waters nanoACQUITY UPLC Quick Start Manual

To characterize the needle seal:
1. In the nanoACQUITY UPLC Console, select Sample Manager from the
system tree.
2. Click Maintain > Characterize > Needle seal.
3. In the Characterize Needle Seal dialog box, click Start. The calibrate
seal operation begins, and the sample manager status displays
"Calibrating seal."
4. When calibration ends, the sample manager status displays "Idle."
5. Click Results to view the needle seal characterization results.
6. Click Close.

Characterizing the needle and sample loop volumes

Whenever you replace the sample loop and/or the sample needle, you must
instruct the system to characterize the volume of the replacement parts. Do
this regardless of whether the sizes of the replacement parts are nominally
the same as those of the original parts or differ from them. Also perform this
procedure when the composition of the weak wash solvent changes.
• Characterizing the loop volume compares the loop's nominal volume to
its measured volume.
• Characterizing the needle volume compares the needle's nominal
volume, 15.0 µL, to its measured volume.
Tip:
Characterizing the system volume is critical to acceptable sample
manager performance.
Requirements:
• Before characterizing the volumes, prime the sample manager, and then
characterize the seal.
• Perform a method setup (MassLynx) with any method that has the same
air gap and sample draw rate that you will be using.
Tip:
This procedure takes approximately 15 minutes.
To characterize the needle and sample loop volumes:
1. In the nanoACQUITY UPLC Console, select Sample Manager from the
system tree.
2-18 Preparing System Hardware