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For typical operating conditions, use the following table as a starting point.
nanoACQUITY UPLC typical operating conditions:
Analytical
Mode
Column ID
Direct
Direct
Direct
Direct
1-pump
trapping
1-pump
trapping
2-pump
trapping
2D-LC
Direct injection
The direct injection mode is used to inject sample directly onto an analytical
column. The trap valve stays in the "elute" position, which routes all flow
through the analytical column. The sample manager post-injection mode can
be programmed in either of two ways:
•
To keep the sample loop in the flow path throughout the run (most
common).
•
To remove the loop from the flow path after time that the user enters.
Single-pump trapping
At nL/min flow rates, samples larger than 2 µL are slow to load onto a
small-bore column. Using single-pump trapping improves sample loading time
by loading samples onto a separate trap column at a higher flow rate while
excess solvent, salts, and impurities elute to waste. After loading, the trap
column is connected to the flow path, and a gradient elutes the sample from
the trap column onto the analytical column, usually at a slower rate.
1-4
System Overview
Analytical
Flow Rate
(µm)
(µL/min)
75
0.20—0.40
100
0.40—0.60
150
0.80—1.20
300
4.00—5.00
75
0.20—0.40
100
0.40—0.60
100
0.40—0.60
75
0.20—0.40
Trap
Trapping
Column
Flow Rate
(ID, µm ×
(µL/min)
length, mm)
n/a
n/a
n/a
n/a
n/a
n/a
n/a
n/a
180 × 20
4.00
180 × 20
4.00
180 × 20
4.00 (up to
50)
180 × 20
3.00—5.00
Injection
Volume (µL)
1—5
1—5
1—10
1—10
1—5
1—5
1—5
5—10
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