Nanoacquity Operating Modes - Waters nanoACQUITY UPLC Quick Start Manual

Smaller-column diameters require lower flow rates, which can encourage
extra-column bandspreading. The system counters this with precise injection
volume capacity and gradient formation, optional detector flow cell volume,
and low-volume connection tubing.
Waters offers nanoACQUITY UPLC columns packed with 1.7-µm, bridged,
ethane-silicon (BEH), hybrid particles as well as conventional reversed phase
packing materials, typically 3- to 5-micron particle sizes. Compared with
traditional HPLC columns, nanoACQUITY UPLC columns deliver superior
resolution and sensitivity in the same run time, or greater sensitivity and
faster run times with equivalent resolution.

nanoACQUITY operating modes

The nanoACQUITY system can operate in direct injection mode or any of four
trapping modes. Trapping improves system performance in several ways:
• Removes salts
• Cleans samples
• Concentrates larger sample volumes
• Decreases sample loading time
Each mode requires a different configuration of solvent managers and
columns:
• Direct injection mode uses the binary solvent manager with an
analytical column.
• Single-pump trapping uses the binary solvent manager with a trap
column and an analytical column.
• Two-pump trapping uses the auxiliary solvent manager and
nanoACQUITY binary solvent manager with a trap column and an
analytical column.
• Online two-dimensional liquid chromatography (2D-LC) trapping, with
salt plugs, uses the auxiliary solvent manager and binary solvent
manager with an ion exchange column, trap column, and analytical
column.
• Off-line 2D-LC trapping requires the CTC PAL MALDI Spotter
Microfraction Collector.
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