Creating The Test Methods; Creating The Instrument Method - Waters nanoACQUITY UPLC Quick Start Manual

Creating the test methods

Follow the steps below to create the methods, setting the parameter values to
match those reflected in the accompanying screen representations. This
method is designed as a rapid test procedure implementing a 150-µm ID
column at high flow rate.

Creating the instrument method

To create the instrument method:
1. Create an instrument method with these binary solvent manager
parameters.
Exception:
table, enter 0.300 µL/min as the flow rate.
Binary solvent manager instrument parameters (General tab):
4-6 Verifying System Operation
If you use the 75 µm ID × 100 mm column, in the gradient