Thermo Scientific Invitrogen E-Gel G8141ST Manuals

Manuals and User Guides for Thermo Scientific Invitrogen E-Gel G8141ST. We have 2 Thermo Scientific Invitrogen E-Gel G8141ST manuals available for free PDF download: User Manual

Thermo Scientific Invitrogen E-Gel G8141ST User Manual (59 pages)

Power Snap Electrophoresis System

Brand: Thermo Scientific | Category: Laboratory Equipment | Size: 2.21 MB

Table of Contents
3
About this Guide

7
- Purpose of the Guide
  7
- Safety
  7
Product Information

8
- Product Description
  8
  - Features
    8
  - Throughput
    8
  - System Components
    8
- Kit Contents and Storage
  9
  - Upon Receiving the Instrument
    9
  - Storage
    9
- Description of Parts
  10
- Using the E-Gel ™ Power Snap Electrophoresis Device
  14
  - Required Materials
    14
  - Prepare Samples
    14
    
    Dilute Samples Containing High Salt
    15
    
    DNA Ladder Preparation Guidelines
    15
  - Prepare Gel
    16
    
    Prepare E-Gel Go! Agarose Gel
    16
  - Sample Loading Guidelines
    17
  - Load Samples
    17
  - Run the Gel
    18
  - Check Status
    19
    
    View Gel
    19
    
    View Gel with Filter Lid Open
    19
  - Modify a Run
    19
    
    Pause the Run
    19
    
    Cancel the Run
    20
    
    Edit Gel Duration
    20
    
    Change to Another Protocol
    20
- Using the E-Gel ™ Power Snap Camera
  21
  - General Guidelines
    21
  - Set up the Camera
    21
    
    Modify Camera Settings
    21
    
    Home Screen
    21
  - Attach the Camera
    22
  - Remove the Camera
    22
  - View Gel
    23
  - Capture Image
    23
    
    Adjust Capture Settings
    23
  - Automatic Image Capture
    24
    
    Cancel Auto Capture
    24
  - Export Image
    24
    
    Export from Capture Screen
    24
    
    Export from Image Gallery
    24
- E-Gel ™ Clonewell ™ II Gels
  25
  - Advantages
    25
  - General Guidelines
    25
  - Prepare Samples
    25
  - Prepare Gel
    25
  - Load Samples
    26
  - Run the Gel
    26
  - Check Status
    26
  - Prepare Wells
    26
  - Collect DNA Fragment
    27
  - Guidelines for Estimating Run Time
    27
  - Troubleshooting
    28
- E-Gel ™ Sizeselect ™ II Gels
  29
  - Advantages
    29
  - General Guidelines
    29
  - Prepare Samples
    29
  - Prepare Gel
    29
  - Load Samples
    30
  - Run the Gel
    30
  - Check Status
    30
  - Prepare Wells
    30
  - Collect DNA Fragment
    31
  - Guidelines for Estimating Run Time
    31
  - Quantitation of Isolated DNA
    33
    
    Troubleshooting
    34
Appendix A

35
- Troubleshooting
  35
Appendix A

35
Appendix B

37
Appendix B System Maintenance

37
- Materials Required
  37
- Cleaning
  37
- Upgrade System Firmware
  37
- Battery Replacement
  38
- Instrument Specifications
  38
Appendix C

40
Appendix C

40
- E-Gel ™ Agarose Gels
  40
- Choosing the Right Gel
  41
- Cassettes
  42
  - Gel Knife
    42
  - EX and NGS Cassettes with a Gel Knife
    42
  - Cleaning and Storage
    43
  - E-Gel ™ Opener
    43
  - Opener
    44
  - Cleaning and Storage
    44
  - E-Gel ™ Agarose Gel Disposal Guidelines
    44
Appendix D

45
Appendix D

45
- Choosing the Right DNA Ladder
  45
Appendix E

46
Appendix E EX Agarose Gels

46
- Non-Denaturing Conditions
  46
- Denaturing Agents
  46
- Denaturing Conditions
  46
Appendix F

47
Appendix F E-Gel ™ Power Snap Blue-Light Transilluminator

47
- Imaging E-Gels on Third Party Gel Imagers
  47
- Agarose Gels
  48
  - SYBR ™ Safe DNA Gel Stain
    48
  - Safety Features
    48
  - Cloning Benefits
    48
  - Disposal
    48
  - Spectrum
    48
  - Visualization
    48
  - SYBR ™ Gold II Gel Stain
    49
  - Disposal
    49
  - Spectrum
    49
  - Visualization
    49
- Instrument Starter Kits
  50
- Accessory Products
  52
- E-Gel ™ Agarose Gels
  52
- Accessory Items
  53
Appendix H

54
Appendix H Safety

54
- Before Starting
  54
- Installing the Instrument
  55
- Electromagnetic Compatibility (EMC) Standards
  55
  - Class a Notice
    55
- Electrical Safety
  56
  - Service Operation Requirements
    56
  - LED (Light-Emitting Diode)
    56
- Explanation of Symbols and Warnings
  57
Appendix I

58
Appendix I

58
- Customer and Technical Support
  58
- Limited Product Warranty
  58

Thermo Scientific Invitrogen E-Gel G8141ST User Manual (61 pages)

Power Snap Electrophoresis System

Brand: Thermo Scientific | Category: Laboratory Equipment | Size: 2.39 MB

Table of Contents
3
About this Guide
7
Purpose of the Guide
7
Safety
7
Product Information
8
Product Description
8
Features
8
Throughput
8
System Components
8
Kit Contents and Storage
9
Upon Receiving the Instrument
9
Storage
9
Description of Parts
10
Front View
10
Parts of the E-Gel ™ Power Snap Electrophoresis Device
11
Parts of the E-Gel ™ Power Snap Camera
12
User Graphical Interface Overview
13
Using the E-Gel ™ Power Snap Electrophoresis Device
14
Required Materials
14
Prepare Samples
14
Dilute Samples Containing High Salt
15
DNA Ladder Preparation Guidelines
15
Prepare Gel
16
Sample Loading Guidelines
16
Load Samples
17
Run the Gel
17
Check Status
18
View Gel
18
View Gel with Filter Lid Open
18
Modify a Run
18
Pause the Run
18
Cancel the Run
19
Edit Gel Duration
19
Change to Another Protocol
19
Using the E-Gel ™ Power Snap Camera
20
General Guidelines
20
Set up the Camera
20
Modify Camera Settings
20
Home Screen
20
Attach the Camera
21
Remove the Camera
21
View Gel
22
Capture Image
22
Adjust Capture Settings
22
Automatic Image Capture
23
Cancel Auto Capture
23
Export Image
23
Export from Capture Screen
23
Export from Image Gallery
24
E-Gel ™ Clonewell ™ II Gels
25
Advantages
25
General Guidelines
25
Prepare Samples
25
Prepare Gel
26
Load Samples
26
Run the Gel
26
Check Status
27
Prepare Wells
27
Collect DNA Fragment
27
Guidelines for Estimating Run Time
28
Troubleshooting
29
E-Gel ™ Sizeselect ™ II Gels
30
Advantages
30
General Guidelines
30
Prepare Samples
30
Prepare Gel
31
Load Samples
31
Run the Gel
31
Check Status
32
Prepare Wells
32
Collect DNA Fragment
32
Guidelines for Estimating Run Time
33
Quantitation of Isolated DNA
35
Troubleshooting
35
Troubleshooting
36
System Maintenance
38
Materials Required
38
Cleaning
38
Battery Replacement
38
Upgrade System Firmware
39
Instrument Specifications
40
Instrument Dimensions and Specifications
40
Electrical Requirements
41
Environmental Requirements
41
E-Gel ™ Agarose Gels
42
Choosing the Right Gel
43
Analytical Gels
43
Gels for Preparative Gel Electrophoresis in Cloning and NGS Applications
43
Other Available Gel Types for Routine Electrophoresis
44
Opening E-Gel ™ Cassettes
44
Gel Knife
44
Open an E-Gel ™ Cassette with a Gel Knife
45
Cleaning and Storage
45
E-Gel ™ Agarose Gel Disposal Guidelines
45
Choosing the Right DNA Ladder
46
Running RNA Samples on E-Gel ™ EX Agarose Gels
47
Denaturing Agents
47
Prepare Ladder
47
Denature Sample
47
Typical Result of RNA Separation on E-Gel ™ EX Agarose Gels
47
E-Gel ™ Power Snap Blue-Light Transilluminator
48
Imaging E-Gels on Third Party Gel Imagers
48
Nucleic Acid Stain Use in E-Gel ™ Agarose Gels
49
SYBR ™ Safe DNA Gel Stain
49
Safety Features
49
Cloning Benefits
49
Disposal
49
Spectrum
49
Visualization
50
SYBR ™ Gold II Gel Stain
50
Disposal
50
Spectrum
50
Instrument Starter Kits
51
E-Gel Agarose Gels
53
Accessory Products
55
Accessory Items
55
Safety
56
Before Starting
56
Installing the Instrument
57
Electromagnetic Compatibility (EMC) Standards
57
Class a Notice
57
Electrical Safety
58
Service Operation Requirements
58
LED (Light-Emitting Diode)
59
Explanation of Symbols and Warnings
59
Customer and Technical Support
60
Limited Product Warranty
60

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Thermo Scientific Invitrogen E-Gel G8141ST Manuals

Thermo Scientific Invitrogen E-Gel G8141ST User Manual (59 pages)

Table of Contents

About this Guide

Product Information

Appendix A

Appendix A

Appendix B

Appendix B System Maintenance

Appendix C

Appendix C

Appendix D

Appendix D

Appendix E

Appendix E EX Agarose Gels

Appendix F

Appendix F E-Gel ™ Power Snap Blue-Light Transilluminator

Appendix H

Appendix H Safety

Appendix I

Appendix I

Thermo Scientific Invitrogen E-Gel G8141ST User Manual (61 pages)

Table of Contents

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