E-Gel ™ Clonewell ™ Ii Gels; Advantages; General Guidelines; Prepare Samples - Thermo Scientific invitrogen E-Gel G8121ST User Manual

E-Gel CloneWell II pre-cast agarose gels are designed for use with the E-Gel
™ ™
and provide a fast, safe, and effective DNA fragment isolation method for DNA cloning workflows.

Advantages

• Target fragments are collected directly from a recovery well. No gel-purification is required.
Contains SYBR
•
efficiency by avoiding UV transillumination.
General
• Load gel within 15 minutes of opening the pouch; run the gel immediately after loading.
guidelines
• Monitor the band of interest carefully as it migrates near the recovery wells. It may be difficult
to see low amounts of DNA in the well.
Important! Always wear Safe Imager
•
lid opened.
For guidance on disposal of used gels, see SYBR
•

Prepare samples

• Prepare up to 25 μL of sample in 1X Sample Loading Buffer (e.g., use 2.5 μL of 10X Sample
Loading Buffer with 22.5 μL total sample).
10X Sample Loading Buffer is provided with E-Gel
Use the indicated amount of DNA per well for single or multiple bands.
•
• Divide samples with higher amounts of DNA across multiple wells.
• Use up to 25 μL total sample volume per well.
Dilute high salt samples (certain restriction enzyme and PCR buffers) 2- to 5-fold.
•
Gel type
E-Gel CloneWell II
™
E-Gel Power Snap Electrophoresis System User Guide
™
Safe DNA stain, eliminating the risk of DNA damage, and improving cloning
™
Amount of DNA per well
Sample with single band
200-800 ng
E-Gel CloneWell
™
Power Snap Electrophoresis Device,
™
Viewing Glasses when viewing the gel with the filter
™
Safe DNA Gel Stain (page 49).
™
Clonewell
™
Sample with multiple bands
800 ng
II gels
™
II Agarose Gels.
™
Total loading
volume
25 μL
25