Check Status; Prepare Wells; Collect Dna Fragment - Thermo Scientific invitrogen E-Gel G8121ST User Manual

Check status

1. Check the gel status by activating the Back
light.
Monitor the gel during the run to avoid the
target fragment missing the recovery well
2. Pause the gel when the band of interest
reaches the reference line (RF) near the row
of recovery wells.
Important: Put on orange Safe Imager
viewing glasses prior to proceeding to
further steps. Reduce ambient light or
work in dark room for better visibility.

Prepare wells

1. Open the filter lid of the E-Gel
Snap Electrophoresis Device and activate
the Back light.
The transilluminator turns off
automatically when the filter lid is opened.
Press Back light to re-activate the blue
light transilluminator.
2. Load 40 μL of deionized water to all
recovery wells. Do not allow water to spill
over the edge of the wells.

Collect DNA fragment

1. Resume the run and carefully observe as
the band of interest fully enters the
recovery well.
2. Stop the gel and recover the sample with a
pipette. Avoid piercing the agarose.
Some residual DNA will remain visible in
the well due to migration into the agarose
at the bottom of the well.
3. Proceed with downstream cloning
workflow. No additional gel-purification is
required.
4. (Optional) Collect additional DNA bands in
the same sample from the recovery well by
adding more water to the recovery well
(see page 27).
5. (Optional) Use the Reverse E-Gel protocol
if the band of interest passes the recovery
well (see page 19).
E-Gel Power Snap Electrophoresis System User Guide
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