Prepare Gel; Load Samples; Run The Gel - Thermo Scientific invitrogen E-Gel G8121ST User Manual

Prepare gel

1. Remove the gel from the package.
2. Gently remove the combs. Do not allow the
combs to bend or create suction in the
wells during removal.
3. Insert gel cassette into the E-Gel
Snap Electrophoresis Device, starting from
the right edge.
4. Press down on the left side of the cassette
to secure it into the device.

Load samples

1. Fill all wells of both rows with 50 μL of
deionized water.
2. Load 25 μL of sample with 1X Sample
Loading Buffer into wells from the bottom
up. Do not damage the gel or introduce
bubbles into the wells.
3. Load 25 μL of ready-to-use E-Gel
Plus Express DNA Ladder into a well.

Run the gel

1. Press Set up run, then select the
CloneWell 0.8% protocol on E-Gel
Snap Electrophoresis Device.
2. Determine the estimated run time. See the
E-Gel
migration pattern for approximate sample
migration time (page 28).
3. Adjust protocol time according to the
expected migration time of the target
fragment to the reference line.
4. Run the gel protocol by pressing Start run.
The run stops automatically after the
programmed time has elapsed.
26
1 Kb Plus Express DNA Ladder
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Power
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1 Kb
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Power
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E-Gel Power Snap Electrophoresis System User Guide
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