Other Available Gel Types For Routine Electrophoresis; Cassettes; Gel Knife; Ex And Ngs Cassettes With A Gel Knife - Thermo Scientific Invitrogen E-Gel G8100 User Manual

Other available gel types for routine electrophoresis

E-Gel
non-denaturing conditions. Use non-denaturing conditions only when checking for RNA quality,
where accurately determining size is not critical. See page 46 for instructions on performing
electrophoresis of RNA samples.
Opening E-Gel
•
Electrophoresis must be complete before opening the E-Gel
•
Photograph the gel before opening the cassette.
•
If you plan to isolate DNA from the E-Gel
fragment immediately after electrophoresis as bands will diffuse within 20 minutes.
•
If you plan to blot the gel, prepare your blotting apparatus before opening the cassette.
•
Important! Before opening the E-Gel

Gel Knife

The Gel Knife (Cat. no. EI9010) is used to open the cassette for E-Gel
Open E-Gel

EX and NGS cassettes with a Gel Knife

™
1. Place the cassette on a flat surface, with the
wells facing upward.
2. Insert the sharp edge of the gel knife into the
groove around the edge of the cassette edge,
then lever the knife up and down to crack the
seal.
3. Unseal the plate by working around the
perimeter of the entire cassette and cracking
the seal for every edge.
4. Remove the top of the gel cassette after all
four sides of the cassette are unsealed.
5. Proceed to downstream application.
If you plan to transfer DNA from the gel by
blotting, only the main running gel is
required. Remove the upper and lower ion
exchange matrix layers and the well areas
with the Gel Knife.
If you plan to purify DNA from the gel,
excise the gel fragment. Transfer the gel slice
to a microcentrifuge tube.
42
™
EX Agarose Gels can be used to run RNA samples. RNA can be run under denaturing or

cassettes

™
™
cassette.
™
agarose gel, open the cassette and excise the gel
™ cassette, put on safety goggles and gloves.
™ ™
EX and E-Gel NGS agarose gels.
E-Gel Power Snap Electrophoresis System User Guide
™
Sharp edge
Insert
Unseal the plate
Remove top plate