Using The E-Gel ™ Power Snap Electrophoresis Device; Required Materials; Prepare Samples - Thermo Scientific Invitrogen E-Gel G8100 User Manual

Using the E-Gel
This section provides instructions for performing electrophoresis using the E-Gel
Electrophoresis Device.
For specific protocols describing the use of E-Gel
For specific protocols describing the use of E-Gel

Required materials

For electrophoresis:
•
E-Gel
•
Safe Imager
•
DNA sample
•
E-Gel
•
E-Gel
weight ladder
•
Optional: 1X E-Gel
•
Optional: E-Gel
For E-Gel
•
E-Gel
•
USB storage device (not included)

Prepare samples

Sample preparation is critical for separation quality. Follow these guidelines for best result.
•
Prepare DNA sample in deionized water or 1X E-Gel
•
Use the indicated amount of DNA per well for single or multiple bands. If you are unsure
how much to use, test a range of concentrations to determine the optimal concentration for
your particular sample. Overloading DNA will cause poor resolution.
Gel Type
E-Gel EX
™
E-Gel EX Double
™
Comb
E-Gel with SYBR
™ ™
Safe
E-Gel Double Comb
™
with SYBR Safe
™
E-Gel Go!
™
E-Gel CloneWell™ II
™
E-Gel SizeSelect II
™
E-Gel NGS
™
14
Power Snap Electrophoresis Device
™
™ Power Snap Electrophoresis Device
™ Viewing Glasses (included)
™
agarose gel cassette (see Choosing the right gel, page 41).
™ DNA Ladder (see Choosing the DNA ladder, page 45) or other appropriate molecular
™ Sample Loading Buffer (Cat No. 10482055)
™ Go! Adapter for E-Gel
gel documentation:
™
™
Power Snap Camera (Cat. No G8300), E-Gel
%
Agarose
Sample with Single Band
1% 0.5–100 ng
2%, 4% 0.5–100 ng
1% 0.5-100 ng
2% 0.5–100 ng
1% 5–300 ng
2%, 4% 5–300 ng
1% 5-300 ng
2% 5-500 ng
1% 0.5–100 ng
0.8% 200-800 ng
2% 1-300 ng
0.8% 20-100 ng
™
CloneWell
™ SizeSelect
™ Power Snap Electrophoresis Device
™
Imager, or other third-party imager.
™ Sample Loading Buffer.
Amount of DNA per Well
Sample with Multiple
Bands
50 ng
50 ng
50 ng
50 ng
500 ng
500 ng
500 ng
500 ng
200 ng
800 ng
500 ng
500 ng
E-Gel Power Snap Electrophoresis System User Guide
™
™
Power Snap
™ II Agarose Gels, see page 25.
™ II Agarose Gels, see page 29.
Total
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Volume
20 µL
10 µL
25 µL