Thermo Scientific Invitrogen E-Gel G8152ST Manuals

Manuals and User Guides for Thermo Scientific Invitrogen E-Gel G8152ST. We have 1 Thermo Scientific Invitrogen E-Gel G8152ST manual available for free PDF download: User Manual

Thermo Scientific Invitrogen E-Gel G8152ST User Manual

Thermo Scientific Invitrogen E-Gel G8152ST User Manual (59 pages)

Power Snap Electrophoresis System

Brand: Thermo Scientific | Category: Laboratory Equipment | Size: 2.21 MB

Table of Contents

Table of Contents
3
About this Guide

7
- Purpose of the Guide
  7
- Safety
  7
Product Information

8
- Product Description
  8
  - Features
    8
  - Throughput
    8
  - System Components
    8
- Kit Contents and Storage
  9
  - Upon Receiving the Instrument
    9
  - Storage
    9
- Description of Parts
  10
- Using the E-Gel ™ Power Snap Electrophoresis Device
  14
  - Required Materials
    14
  - Prepare Samples
    14
    
    Dilute Samples Containing High Salt
    15
    
    DNA Ladder Preparation Guidelines
    15
  - Prepare Gel
    16
    
    Prepare E-Gel Go! Agarose Gel
    16
  - Sample Loading Guidelines
    17
  - Load Samples
    17
  - Run the Gel
    18
  - Check Status
    19
    
    View Gel
    19
    
    View Gel with Filter Lid Open
    19
  - Modify a Run
    19
    
    Pause the Run
    19
    
    Cancel the Run
    20
    
    Edit Gel Duration
    20
    
    Change to Another Protocol
    20
- Using the E-Gel ™ Power Snap Camera
  21
  - General Guidelines
    21
  - Set up the Camera
    21
    
    Modify Camera Settings
    21
    
    Home Screen
    21
  - Attach the Camera
    22
  - Remove the Camera
    22
  - View Gel
    23
  - Capture Image
    23
    
    Adjust Capture Settings
    23
  - Automatic Image Capture
    24
    
    Cancel Auto Capture
    24
  - Export Image
    24
    
    Export from Capture Screen
    24
    
    Export from Image Gallery
    24
- E-Gel ™ Clonewell ™ II Gels
  25
  - Advantages
    25
  - General Guidelines
    25
  - Prepare Samples
    25
  - Prepare Gel
    25
  - Load Samples
    26
  - Run the Gel
    26
  - Check Status
    26
  - Prepare Wells
    26
  - Collect DNA Fragment
    27
  - Guidelines for Estimating Run Time
    27
  - Troubleshooting
    28
- E-Gel ™ Sizeselect ™ II Gels
  29
  - Advantages
    29
  - General Guidelines
    29
  - Prepare Samples
    29
  - Prepare Gel
    29
  - Load Samples
    30
  - Run the Gel
    30
  - Check Status
    30
  - Prepare Wells
    30
  - Collect DNA Fragment
    31
  - Guidelines for Estimating Run Time
    31
  - Quantitation of Isolated DNA
    33
    
    Troubleshooting
    34
Appendix A

35
- Troubleshooting
  35
Appendix A

35
Appendix B

37
Appendix B System Maintenance

37
- Materials Required
  37
- Cleaning
  37
- Upgrade System Firmware
  37
- Battery Replacement
  38
- Instrument Specifications
  38
Appendix C

40
Appendix C

40
- E-Gel ™ Agarose Gels
  40
- Choosing the Right Gel
  41
- Cassettes
  42
  - Gel Knife
    42
  - EX and NGS Cassettes with a Gel Knife
    42
  - Cleaning and Storage
    43
  - E-Gel ™ Opener
    43
  - Opener
    44
  - Cleaning and Storage
    44
  - E-Gel ™ Agarose Gel Disposal Guidelines
    44
Appendix D

45
Appendix D

45
- Choosing the Right DNA Ladder
  45
Appendix E

46
Appendix E EX Agarose Gels

46
- Non-Denaturing Conditions
  46
- Denaturing Agents
  46
- Denaturing Conditions
  46
Appendix F

47
Appendix F E-Gel ™ Power Snap Blue-Light Transilluminator

47
- Imaging E-Gels on Third Party Gel Imagers
  47
- Agarose Gels
  48
  - SYBR ™ Safe DNA Gel Stain
    48
  - Safety Features
    48
  - Cloning Benefits
    48
  - Disposal
    48
  - Spectrum
    48
  - Visualization
    48
  - SYBR ™ Gold II Gel Stain
    49
  - Disposal
    49
  - Spectrum
    49
  - Visualization
    49
- Instrument Starter Kits
  50
- Accessory Products
  52
- E-Gel ™ Agarose Gels
  52
- Accessory Items
  53
Appendix H

54
Appendix H Safety

54
- Before Starting
  54
- Installing the Instrument
  55
- Electromagnetic Compatibility (EMC) Standards
  55
  - Class a Notice
    55
- Electrical Safety
  56
  - Service Operation Requirements
    56
  - LED (Light-Emitting Diode)
    56
- Explanation of Symbols and Warnings
  57
Appendix I

58
Appendix I

58
- Customer and Technical Support
  58
- Limited Product Warranty
  58

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