Dilute Samples Containing High Salt; Dna Ladder Preparation Guidelines - Thermo Scientific Invitrogen E-Gel G8100 User Manual

Dilute E-Gel
100 mM KCl, 10 mM acetate ions, or 10 mM EDTA (i.e., certain restriction enzyme and PCR
samples
buffers) cause loss of resolution on E-Gel
containing
Dilute samples as suggested below:
high salt
•
•
•
DNA ladder •
Dilute the ladder accordingly with deionized water or 1X E-Gel
preparation
•
Use the indicated amount of ladder per well. Overloading the ladder will result in distorted
guidelines
or incomplete band separation.
E-Gel DNA E-Gel
™ ™
Ladder EX
E-Gel Ultra
™
4 μL
Low Range
(100 ng)
DNA Ladder
E-Gel 50 bp
™
2 μL
DNA Ladder
(50 ng)
E-Gel 1 Kb
™
2 μL
Plus DNA
(50 ng)
Ladder
E-Gel 1 Kb 2 μL
™
Plus Express (80 ng)
E-Gel Sizing
™
—
DNA Ladder
E-Gel Low
™
5 μL
Range
(87.5
Quantitative
ng)
DNA Ladder
E-Gel 96 3 µL
™
High Range (15 ng)
E-G el Power Snap Electroph oresis System User Guide
™
™ EX gels are sensitive to high salt and EDTA content. Samples containing ≥50 mM NaCl,
Dilute E-Gel ™ EX agarose gels 10-30 fold.
™ ™
Dilute E-Gel SYBR
Dilute E-Gel ™ EX Double Comb agarose gels 10-30 fold.
E-Gel EX E-Gel
™ ™
Double SYBR
™
Comb
20 μL
—
(500 ng)
2 μL 20 μL
(50 ng) (500 ng)
2 μL 20 μL
(50 ng) (500 ng)
20 μL
—
(800 ng)
— —
3 μL 20 μL
(52.5 ng) (350 ng)
3 µL 20 µL
(15 ng) (100 ng)
™ agarose gels.
Safe agarose gels 2-10 fold.
with E-Gel EX E-Gel
™
Safe Double CloneWell
Comb with
SYBR
™
Safe
—
20 μL
(500 ng)
20 μL 25 μL
(500 ng) (625 ng)
20 μL 25 μL
(800 ng) (1,000 ng)
—
20 μL
(350 ng)
20 µL
(100 ng)
™
Sample Loading Buffer.
E-Gel E-Gel
™ ™
SizeSelect Go!
II II
— — —
10 μL
2 μL (50
—
(250
ng)
ng)
10 μL
— (250
ng)
5 μL
— (200
ng)
25 μL 10 μL
—
(50 ng) (20 ng)
10 μL
— — (175
ng)
— — —
E-Gel
™ ™
NGS
—
—
—
—
25 µL
(50 ng)
—
—
15