Sample Loading Guidelines; Load Samples - Thermo Scientific Invitrogen E-Gel G8100 User Manual

Sample loading guidelines

•
Use the recommended total loading volume for each gel type. Do not load more than
recommended amount of DNA sample or ladder per well.
•
Load deionized water into all empty wells.
•
Keep all sample volumes uniform. If you do not have enough samples to load all the wells of
the gel, load an identical volume of deionized water into any empty wells. Prepare your
samples by adding E-Gel
amount of DNA to bring the total required sample volume.
•
Avoid introducing bubbles while loading. Bubbles can cause band distortion.
E-Gel
E-Gel
E-Gel
E-Gel
E-Gel
E-Gel
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E-Gel

Load samples

1. Load prepared samples. Keep all sample
volumes uniform.
2. Load prepared DNA ladder.
3. Load 1X E-Gel Sample Loading Buffer or
deionized water in all empty wells.
4. Run gels within 1 minute after loading
samples.
E-G el Power Snap Electroph oresis System User Guide
™
™
Gel type
EX
™
with SYBR Safe
™ ™
EX Double Comb
™
Double Comb with SYBR
™
Go!
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CloneWell II
™
SizeSelect II
™
NGS
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1X Sample Loading Buffer or deionized water to the required
Safe
™
Total loading volume
20 μL
10 μL
25 μL
25 μL
20 μL
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