Check Status; Prepare Wells; Collect Dna Fragment - Thermo Scientific invitrogen E-Gel G8121ST User Manual

Check status

1. Check the gel status by activating the Back
light.
Monitor the gel during the run to avoid the
target fragment missing the recovery well
2. Pause the gel when the reference band of
the DNA ladder reaches the reference line
(RF) near the row of recovery wells.
Important: Put on orange Safe Imager
viewing glasses prior to proceeding to
further steps. Reduce ambient light or
work in dark room for better visibility.

Prepare wells

1. Open the filter lid of the E-Gel
Snap Electrophoresis Device and activate
the Back light.
The transilluminator turns off
automatically when the filter lid is opened.
Press Back light to re-activate the blue
light transilluminator.
2. Carefully remove all liquid from the
recovery wells.
3. Load 50 μL of nuclease-free water to all
recovery wells. Do not allow water to spill
over the edge of the wells.

Collect DNA fragment

1. Resume the run and carefully observe as
the reference band enters the recovery well.
Important: See NGS library size selection
reference to determine when to collect
samples of specific target library length.
2. Stop the gel and recover the sample with a
pipette. Avoid piercing the agarose.
Some residual DNA will remain visible in
the well due to migration into the agarose
at the bottom of the well.
3. Proceed with downstream NGS workflow.
4. (Optional) Use the Reverse E-Gel protocol
if the band of interest passes the recovery
well (see page 19).
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