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Invitrogen E-Gel G8151ST
Thermo Scientific Invitrogen E-Gel G8151ST Manuals
Manuals and User Guides for Thermo Scientific Invitrogen E-Gel G8151ST. We have
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Thermo Scientific Invitrogen E-Gel G8151ST manual available for free PDF download: User Manual
Thermo Scientific Invitrogen E-Gel G8151ST User Manual (59 pages)
Power Snap Electrophoresis System
Brand:
Thermo Scientific
| Category:
Laboratory Equipment
| Size: 2.21 MB
Table of Contents
Table of Contents
3
About this Guide
7
Purpose of the Guide
7
Safety
7
Product Information
8
Product Description
8
Features
8
Throughput
8
System Components
8
Kit Contents and Storage
9
Upon Receiving the Instrument
9
Storage
9
Description of Parts
10
Front View
10
Parts of the E-Gel ™ Power Snap Electrophoresis Device
11
Parts of the E-Gel ™ Power Snap Camera
12
User Graphical Interface Overview
13
Using the E-Gel ™ Power Snap Electrophoresis Device
14
Required Materials
14
Prepare Samples
14
Dilute Samples Containing High Salt
15
DNA Ladder Preparation Guidelines
15
Prepare Gel
16
Prepare E-Gel Go! Agarose Gel
16
Sample Loading Guidelines
17
Load Samples
17
Run the Gel
18
Check Status
19
View Gel
19
View Gel with Filter Lid Open
19
Modify a Run
19
Pause the Run
19
Cancel the Run
20
Edit Gel Duration
20
Change to Another Protocol
20
Using the E-Gel ™ Power Snap Camera
21
General Guidelines
21
Set up the Camera
21
Modify Camera Settings
21
Home Screen
21
Attach the Camera
22
Remove the Camera
22
View Gel
23
Capture Image
23
Adjust Capture Settings
23
Automatic Image Capture
24
Cancel Auto Capture
24
Export Image
24
Export from Capture Screen
24
Export from Image Gallery
24
E-Gel ™ Clonewell ™ II Gels
25
Advantages
25
General Guidelines
25
Prepare Samples
25
Prepare Gel
25
Load Samples
26
Run the Gel
26
Check Status
26
Prepare Wells
26
Collect DNA Fragment
27
Guidelines for Estimating Run Time
27
Troubleshooting
28
E-Gel ™ Sizeselect ™ II Gels
29
Advantages
29
General Guidelines
29
Prepare Samples
29
Prepare Gel
29
Load Samples
30
Run the Gel
30
Check Status
30
Prepare Wells
30
Collect DNA Fragment
31
Guidelines for Estimating Run Time
31
Quantitation of Isolated DNA
33
Troubleshooting
34
Appendix A
35
Troubleshooting
35
Appendix A
35
Appendix B
37
Appendix B System Maintenance
37
Materials Required
37
Cleaning
37
Upgrade System Firmware
37
Battery Replacement
38
Instrument Specifications
38
Instrument Dimensions and Specifications
38
Electrical Requirements
39
Environmental Requirements
39
Appendix C
40
Appendix C
40
E-Gel ™ Agarose Gels
40
Choosing the Right Gel
41
Analytical Gels
41
Gels for Preparative Gel Electrophoresis in Cloning and NGS Applications
41
Other Available Gel Types for Routine Electrophoresis
42
Cassettes
42
Gel Knife
42
EX and NGS Cassettes with a Gel Knife
42
Cleaning and Storage
43
E-Gel ™ Opener
43
Opener
44
Cleaning and Storage
44
E-Gel ™ Agarose Gel Disposal Guidelines
44
Appendix D
45
Appendix D
45
Choosing the Right DNA Ladder
45
Appendix E
46
Appendix E EX Agarose Gels
46
Non-Denaturing Conditions
46
Denaturing Agents
46
Denaturing Conditions
46
Appendix F
47
Appendix F E-Gel ™ Power Snap Blue-Light Transilluminator
47
Imaging E-Gels on Third Party Gel Imagers
47
Agarose Gels
48
SYBR ™ Safe DNA Gel Stain
48
Safety Features
48
Cloning Benefits
48
Disposal
48
Spectrum
48
Visualization
48
SYBR ™ Gold II Gel Stain
49
Disposal
49
Spectrum
49
Visualization
49
Instrument Starter Kits
50
Accessory Products
52
E-Gel ™ Agarose Gels
52
Accessory Items
53
Appendix H
54
Appendix H Safety
54
Before Starting
54
Installing the Instrument
55
Electromagnetic Compatibility (EMC) Standards
55
Class a Notice
55
Electrical Safety
56
Service Operation Requirements
56
LED (Light-Emitting Diode)
56
Explanation of Symbols and Warnings
57
Appendix I
58
Appendix I
58
Customer and Technical Support
58
Limited Product Warranty
58
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