E-Gel ™ Sizeselect ™ Ii Gels; Advantages; General Guidelines; Prepare Samples - Thermo Scientific invitrogen E-Gel G8121ST User Manual

E-Gel SizeSelect II 2% Agarose Gels are designed for use with the E-Gel
™ ™
provide a fast and convenient method for DNA fragment library size selection as part of NGS library preparation
workflows.

Advantages

• Target fragments are collected directly from a recovery well.
Contains highly-sensitive SYBR
•
1.5 ng/band of DNA.
General Load gel within 15 minutes of opening the pouch; run the gel after loading.
•
guidelines
Important! Always wear Safe Imager
•
lid opened.
For guidance on disposal of used gels, see SYBR
•

Prepare samples

Prepare up to 25 μL of sample in 1X Sample Loading Buffer (e.g., use 2.5 μL of 10X Sample
•
Loading Buffer with 22.5 μL total sample).
10X Sample Loading Buffer is provided with E-Gel
• Use the indicated amount of DNA per well for single or multiple bands.
Do not exceed 1 μg for sheared DNA.
•
Divide samples with higher amounts of DNA across multiple wells.
•
• Use up to 25 μL total sample volume per well.
Dilute high salt samples (certain restriction enzyme and PCR buffers) 2- to 5-fold.
•
Gel type
E-Gel SizeSelect II
™
30
Amount of DNA per well
Sample with single band
1–300 ng
E-Gel SizeSelect
™
Power Snap Electrophoresis Device, and
™
Gold II nucleic acid stain that allows detection down to
™
Viewing Glasses when viewing the gel with the filter
™
Gold II DNA Stain (page 50).
™
SizeSelect
™
Sample with multiple bands
300 ng
E-Gel Power Snap Electrophoresis System User Guide
™
II gels
™
II Agarose Gels.
™
Total loading
volume
25 μL