E-Gel
Power Snap Blue-Light Transilluminator
™
To monitor sample separation right at laboratory bench, the E-Gel
integrated blue-light LED source with emission maximum at 465 nm. This enables real-time monitoring of samples
running on E-Gel
™
agarose gels that are pre-stained with SYBR Safe
The light from a LED source within the transilluminator passes through a blue filter producing a single intensity
signal at approximately 465 nm, effective for the excitation of SYBR
gel stain and SYBR Gold. Sensitivity obtained using this instrument is comparable to that obtained with a standard
UV transilluminator.
The E-Gel
™
Power Snap Electrophoresis Device transilluminator is designed for viewing E-Gel
™
gels, E-Gel
EX gels, E-Gel
The use of blue-light transillumination is advantageous over the UV, as it does not require UV protective equipment
during use. In preparative gel electrophoresis blue-light transillumination results in dramatically increased cloning
efficiencies compared to UV transillumination.
Important! Do not look directly at blue-light transilluminator surface. Make sure the filter lid is closed when the blue
light is on. When working with opened filter cover, always use E-Gel
Imaging E-Gels
on Third Party
Gel Imagers
E-G el
Power Snap Electroph oresis System User Guide
™
™
™
CloneWell
II gels, E-Gel
For E-Gel
™
agarose gel imaging on other commercially available imaging devices follow user
guides provided by the supplier. Instruments with an excitation source in the UV range or
between 470–530 nm may also be used with the proper filter. Contact your instrument
manufacturer for advice.
™
Power Snap Electrophoresis Device has an
™
or SYBR Gold II DNA stains.
™
DNA-binding dyes such as SYBR
™
™
SizeSelect
II, and E-Gel
™
Safe Imager
Appendix F
™
Safe DNA
™
with SYBR
™
Go! gels.
™
viewing glasses.
™
Safe
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