E-Gel ™ Sizeselect ™ Ii Gels; Advantages; General Guidelines; Prepare Samples - Thermo Scientific Invitrogen E-Gel G8100 User Manual

™ ™
E-Gel SizeSelect II 2% Agarose Gels are designed for use with the E-Gel
provide a fast and convenient method for DNA fragment library size selection as part of NGS library preparation
workflows.

Advantages

•
Target fragments are collected directly from a recovery well.
•
Contains highly-sensitive SYBR
0.5 ng/band of DNA.
General
•
Load gel within 15 minutes of opening the pouch; run the gel immediately after loading.
guidelines
•
Important! Always wear Safe Imager
lid opened.
•
For guidance on disposal of used gels, see SYBR

Prepare samples

•
Prepare up to 25 μL of sample in 1X Sample Loading Buffer (e.g., use 2.5 μL of 10X Sample
Loading Buffer with 22.5 μL total sample).
10X Sample Loading Buffer is provided with E-Gel
•
Use the indicated amount of DNA per well for single or multiple bands.
•
Do not exceed 1 μg for sheared DNA.
•
Divide samples with higher amounts of DNA across multiple wells.
•
Use up to 25 μL total sample volume per well.
•
Dilute high salt samples (certain restriction enzyme and PCR buffers) 2- to 5-fold.
Gel type
E-Gel SizeSelect II
™

Prepare gel

1. Remove the gel from the package.
2. Gently remove the combs. Do not allow the
combs to bend or create suction in the
wells during removal.
3. Insert gel cassette into the E-Gel
Snap Electrophoresis Device, starting from
the right edge.
4. Press down on the left side of the cassette
to secure it into the device.
E-G el Power Snap Electroph oresis System User Guide
™
Amount of DNA per well
Sample with single band
1-300 ng
E-Gel SizeSelect
™
™
Power Snap Electrophoresis Device and
™ Gold II nucleic acid stain that allows detection down to
™ Viewing Glasses when viewing the gel with the filter
™
Gold II DNA Stain (page 49).
™ SizeSelect
Sample with multiple bands
500 ng
™ Power
II gels
™
™ II Agarose Gels.
Total loading
volume
25 μL
29