Load Samples; Run The Gel; Check Status; Prepare Wells - Thermo Scientific Invitrogen E-Gel G8100 User Manual

Load samples

1. Fill all wells of both rows with 50 μL of
deionized water.
2. Load 25 μL of sample with 1X Sample
Loading Buffer into wells from the bottom
up. Do not damage the gel or introduce
bubbles into the wells.
3. Load 25 μL of ready-to-use E-Gel
Plus Express DNA Ladder into a well.

Run the gel

1. Press Set up run, then select the
CloneWell 0.8% protocol on E-Gel
Snap Electrophoresis Device.
2. Determine the estimated run time. See the
E-Gel
migration pattern for approximate sample
migration time (page 27).
3. Adjust protocol time according to the
expected migration time of the target
fragment to the reference line.
4. Run the gel protocol by pressing Start run.
The run stops automatically after the
programmed time has elapsed.

Check status

1. Check the gel status by activating the Back
light.
Monitor the gel during the run to avoid the
target fragment missing the recovery well
2. Pause the gel when the band of interest
reaches the reference line (RF) near the row
of recovery wells.
Important: Put on orange Safe Imager
viewing glasses prior to proceeding to
further steps. Reduce ambient light or
work in dark room for better visibility.

Prepare wells

1. Open the filter lid of the E-Gel
Snap Electrophoresis Device and activate
the Back light.
The transilluminator turns off
automatically when the filter lid is opened.
Press Back light to re-activate the blue
light transilluminator.
2. Load 40 μL of deionized water to all
recovery wells. Do not allow water to spill
over the edge of the wells.
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™ 1 Kb Plus Express DNA Ladder
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E-Gel Power Snap Electrophoresis System User Guide
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