Collect Dna Fragment; Guidelines For Estimating Run Time - Thermo Scientific Invitrogen E-Gel G8100 User Manual

Collect DNA fragment

1. Resume the run and carefully observe as
the band of interest fully enters the
recovery well.
2. Stop the gel and recover the sample with a
pipette. Avoid piercing the agarose.
Some residual DNA will remain visible in
the well due to migration into the agarose
at the bottom of the well.
3. Proceed with downstream cloning
workflow. No additional gel-purification is
required.
4. (Optional) Collect additional DNA bands in
the same sample from the recovery well by
adding more water to the recovery well
(see page 26).
5. (Optional) Use the Reverse E-Gel protocol
if the band of interest passes the recovery
well (see page 20).

Guidelines for estimating run time

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E-Gel 1 Kb Plus Express DNA Ladder migration pattern
™
Ladder
E-G el Power Snap Electroph oresis System User Guide
™
Refer to the E-Gel ™ 1 Kb Plus Express DNA Ladder migration pattern table to estimate
target DNA run time to the reference line.
The run times indicated in the table are estimates. Monitor your gel in real time during
the run to ensure the sample does not pass the recovery well.
Identically sized bands in different wells may migrate differently.
DNA fragment size, amount, and salt content can affect migration rates.
Fragment size
5000 bp
3000 bp
2000 bp
1500 bp
1000 bp
750 bp
500 bp
300 bp
100 bp
DNA amount (per 25 μL)
100 ng
100 ng
100 ng
160 ng
90 ng
90 ng
180 ng
90 ng
90 ng
Migration time to
reference line
~27.5 min
~23 min
~20.5 min
~19 min
~17 min
~16 min
~15 min
~14 min
~13 min
27