Sybr ™ Gold Ii Gel Stain; Disposal; Spectrum; Visualization - Thermo Scientific Invitrogen E-Gel G8100 User Manual

SYBR Gold II Gel Stain
™
SYBR
E-Gel Go! agarose gels. This gel stain has high sensitivity, with detection down to 0.5 ng/band of
DNA. This fluorescent nucleic acid stain can be viewed by blue light transilluminator, significantly
reducing DNA damage that can reduce cloning efficiency.

Disposal

Dispose E-Gel
same manner as ethidium bromide containing gels. Contact your safety office or local municipality
for appropriate disposal in your community.

Spectrum

When bound to nucleic acids, the proprietary nucleic acid stain in E-Gel
and E-Gel
maximum at 522 nm (see figure below).
Normalized fluorescence excitation and emission spectra of proprietary DNA gel stain in E-Gel
EX, E-Gel

Visualization

For quick visualization and documentation of SYBR
E-Gel
Alternatively, use a blue light transilluminator or a standard UV transilluminator.
E-G el Power Snap Electroph oresis System User Guide
™
™
Gold II gel stain has been specifically developed for E-Gel
™ EX, E-Gel ™ SizeSelect
™
Go! agarose gels has fluorescence excitation maxima at 490 nm, and an emission
™ SizeSelect ™ and E-Gel
™ Power Snap Camera.
™ and E-Gel ™ Go! agarose gels as hazardous waste in the
™ Go! agarose gels, determined in the presence of DNA.
™ Gold II stained E-Gel
™ ™ ™
EX, E-Gel SizeSelect
™ ™
EX, E-Gel SizeSelect
™ agarose gels use
II and
™
™
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