E-Gel ™ Power Snap Blue-Light Transilluminator; Imaging E-Gels On Third Party Gel Imagers - Thermo Scientific invitrogen E-Gel G8121ST User Manual

E-Gel Power Snap Blue-Light Transilluminator
™
To monitor sample separation right at laboratory bench, the E-Gel
integrated blue-light LED source with emission maximum at 465 nm. This enables real-time monitoring of samples
running on E-Gel agarose gels that are pre-stained with SYBR Safe
™
The light from a LED source within the transilluminator passes through a blue filter producing a single intensity
signal at approximately 465 nm, effective for the excitation of SYBR
gel stain and SYBR Gold. Sensitivity obtained using this instrument is comparable to that obtained with a standard
UV transilluminator.
The E-Gel Power Snap Electrophoresis Device transilluminator is designed for viewing E-Gel
™
gels, E-Gel EX gels, E-Gel
™
The use of blue-light transillumination is advantageous over the UV, as it does not require UV protective equipment
during use. In preparative gel electrophoresis blue-light transillumination results in dramatically increased cloning
efficiencies compared to UV transillumination.
Important! Do not look directly at blue-light transilluminator surface. Make sure the filter lid is closed when the blue
light is on. When working with opened filter cover, always use E-Gel
Imaging E-Gels
For E-Gel
guides provided by the supplier. Instruments with an excitation source in the UV range or
on Third Party
between 470–530 nm may also be used with the proper filter. Contact your instrument
Gel Imagers
manufacturer for advice.
48
CloneWell II gels, and E-Gel
™ ™
agarose gel imaging on other commercially available imaging devices follow user
™
Power Snap Electrophoresis Device has an
™
or SYBR Gold II DNA stains.
™
DNA-binding dyes such as SYBR
™
SizeSelect II gels.
™ ™
Safe Imager
™ ™
E-Gel Power Snap Electrophoresis System User Guide
™
Appendix F
Safe DNA
™
with SYBR Safe
™ ™
viewing glasses.