Thermo Scientific invitrogen E-Gel G8122ST Manuals

Manuals and User Guides for Thermo Scientific invitrogen E-Gel G8122ST. We have 1 Thermo Scientific invitrogen E-Gel G8122ST manual available for free PDF download: User Manual

Thermo Scientific invitrogen E-Gel G8122ST User Manual (61 pages)

Power Snap Electrophoresis System

Brand: Thermo Scientific | Category: Laboratory Equipment | Size: 2.39 MB

Table of Contents
3
About this Guide
7
Purpose of the Guide
7
Safety
7
Product Information
8
Product Description
8
Features
8
Throughput
8
System Components
8
Kit Contents and Storage
9
Upon Receiving the Instrument
9
Storage
9
Description of Parts
10
Front View
10
Parts of the E-Gel ™ Power Snap Electrophoresis Device
11
Parts of the E-Gel ™ Power Snap Camera
12
User Graphical Interface Overview
13
Using the E-Gel ™ Power Snap Electrophoresis Device
14
Required Materials
14
Prepare Samples
14
Dilute Samples Containing High Salt
15
DNA Ladder Preparation Guidelines
15
Prepare Gel
16
Sample Loading Guidelines
16
Load Samples
17
Run the Gel
17
Check Status
18
View Gel
18
View Gel with Filter Lid Open
18
Modify a Run
18
Pause the Run
18
Cancel the Run
19
Edit Gel Duration
19
Change to Another Protocol
19
Using the E-Gel ™ Power Snap Camera
20
General Guidelines
20
Set up the Camera
20
Modify Camera Settings
20
Home Screen
20
Attach the Camera
21
Remove the Camera
21
View Gel
22
Capture Image
22
Adjust Capture Settings
22
Automatic Image Capture
23
Cancel Auto Capture
23
Export Image
23
Export from Capture Screen
23
Export from Image Gallery
24
E-Gel ™ Clonewell ™ II Gels
25
Advantages
25
General Guidelines
25
Prepare Samples
25
Prepare Gel
26
Load Samples
26
Run the Gel
26
Check Status
27
Prepare Wells
27
Collect DNA Fragment
27
Guidelines for Estimating Run Time
28
Troubleshooting
29
E-Gel ™ Sizeselect ™ II Gels
30
Advantages
30
General Guidelines
30
Prepare Samples
30
Prepare Gel
31
Load Samples
31
Run the Gel
31
Check Status
32
Prepare Wells
32
Collect DNA Fragment
32
Guidelines for Estimating Run Time
33
Quantitation of Isolated DNA
35
Troubleshooting
35
Troubleshooting
36
System Maintenance
38
Materials Required
38
Cleaning
38
Battery Replacement
38
Upgrade System Firmware
39
Instrument Specifications
40
Instrument Dimensions and Specifications
40
Electrical Requirements
41
Environmental Requirements
41
E-Gel ™ Agarose Gels
42
Choosing the Right Gel
43
Analytical Gels
43
Gels for Preparative Gel Electrophoresis in Cloning and NGS Applications
43
Other Available Gel Types for Routine Electrophoresis
44
Opening E-Gel ™ Cassettes
44
Gel Knife
44
Open an E-Gel ™ Cassette with a Gel Knife
45
Cleaning and Storage
45
E-Gel ™ Agarose Gel Disposal Guidelines
45
Choosing the Right DNA Ladder
46
Running RNA Samples on E-Gel ™ EX Agarose Gels
47
Denaturing Agents
47
Prepare Ladder
47
Denature Sample
47
Typical Result of RNA Separation on E-Gel ™ EX Agarose Gels
47
E-Gel ™ Power Snap Blue-Light Transilluminator
48
Imaging E-Gels on Third Party Gel Imagers
48
Nucleic Acid Stain Use in E-Gel ™ Agarose Gels
49
SYBR ™ Safe DNA Gel Stain
49
Safety Features
49
Cloning Benefits
49
Disposal
49
Spectrum
49
Visualization
50
SYBR ™ Gold II Gel Stain
50
Disposal
50
Spectrum
50
Instrument Starter Kits
51
E-Gel Agarose Gels
53
Accessory Products
55
Accessory Items
55
Safety
56
Before Starting
56
Installing the Instrument
57
Electromagnetic Compatibility (EMC) Standards
57
Class a Notice
57
Electrical Safety
58
Service Operation Requirements
58
LED (Light-Emitting Diode)
59
Explanation of Symbols and Warnings
59
Customer and Technical Support
60
Limited Product Warranty
60

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Thermo Scientific invitrogen E-Gel G8122ST User Manual (61 pages)

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