Other Available Gel Types For Routine Electrophoresis; Opening E-Gel ™ Cassettes; Gel Knife - Thermo Scientific invitrogen E-Gel G8121ST User Manual

Other available gel types for routine electrophoresis

E-Gel
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non-denaturing conditions. Use non-denaturing conditions only when checking for RNA quality,
where accurately determining size is not critical. See page 47 for instructions on performing
electrophoresis of RNA samples.
Opening E-Gel
Electrophoresis must be complete before opening the E-Gel
•
• Photograph the gel before opening the cassette.
• If you plan to isolate DNA from the E-Gel
fragment immediately after electrophoresis as bands will diffuse within 20 minutes.
• If you plan to blot the gel, prepare your blotting apparatus before opening the cassette.
Important! Before opening the E-Gel
•

Gel Knife

The Gel Knife (Cat. No. EI9010) is used to open the cassette for various types of E-Gel
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EX Agarose Gels can be used to run RNA samples. RNA can be run under denaturing or
cassettes
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cassette.
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agarose gel, open the cassette and excise the gel
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cassette, put on safety goggles and gloves.
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E-Gel Power Snap Electrophoresis System User Guide
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agarose gels (see page 43).
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Sharp edge