Opener; Cleaning And Storage; E-Gel ™ Agarose Gel Disposal Guidelines - Thermo Scientific Invitrogen E-Gel G8100 User Manual

Open the E-Gel cassette with an E-Gel
™
Cleaning
After use, clean the E-Gel
and plastic from the platform. Use a squirt bottle and wipe the platform dry with a clean tissue. Do
and storage
not insert your fingers into the area housing the blades, and do not immerse the E-Gel
water as the blades may rust. Store the E-Gel
E-Gel agarose gel disposal guidelines
™
44
™
1. Place the E-Gel Opener on a flat surface, with the knob extending off the edge of the
laboratory bench and facing the user.
™
2. Set the E-Gel Opener to its widest open position by turning the knob counterclockwise.
™
3. Insert the E-Gel into the E-Gel
are aligned with the blades. Position the cassette such that the two sides fit into the
grooves housing the blades.
4. Turn the knob clockwise to bring the blades in contact with the cassette. As the knob is
tightened, you will hear a series of pops.
5. Continue to turn the knob until the resistance increases. Stop turning the knob as soon as
the cassette begins to lift off the surface of the platform. Two sides of the cassette will
now be unsealed.
:
Note Once you observe the cassette begins to lift off the surface of the platform, do not
continue to tighten the knob as you will damage the E-Gel
6. Unscrew the knob and remove the cassette. You may have to carefully work the cassette
from the housing because the cassette fits snugly in the recessed groove
7. Turn the cassette 90° and re-insert the cassette into the E-Gel
remaining sides.
8. Repeat steps 4–5 to break the two remaining seals.
9. Unscrew the knob and carefully remove the E-Gel
should be unsealed. If not, repeat Steps 2–5 as necessary.
™ Opener with mild detergent and water to remove any excess agarose
• ™
Discard E-Gel EX Agarose Gels, E-Gel
Agarose Gels as hazardous waste.
•
™
SYBR Safe stain is not classified as hazardous waste under US Federal regulations, but
contact your safety office for appropriate disposal methods (see page 48).

Opener

™
™
Opener so that two opposing sides of the gel cassette
™
Opener at room temperature.
™
SizeSelect
E-Gel Power Snap Electrophoresis System User Guide
™
™ agarose gel.
™ Opener to open the two
™ cassette. The 4 sides of the cassette
™
Agarose Gels, and E-Gel
™ Opener in
™
Go!