Table Of Contents - Infors HT Labfors 5 Cell User Instructions

27 May 2014
1 General Information .............................................................5
1.1 About this Document ...................................................5
1.2 About the Device Option ..............................................5
2 Safety ...................................................................................6
2.1 Personnel ....................................................................6
2.2 Personal Protective Equipment ....................................7
3 Technical Data .....................................................................8
3.1 Stirrer Drive .................................................................8
3.2 Temperature ................................................................8
4 Setup and Function ........................................................... 10
4.1 Overview Labfors 5 Cell with Single-Use Vessel
Option ....................................................................... 10
4.1.1 Single-Use Vessel ....................................... 11
4.1.2 Heating and Temperature Measurement ..... 11
4.1.3 Stirrer Drive ................................................ 12
4.1.4 Connection with Gassing and Fluid
Addition ...................................................... 13
4.1.5 Cultivation and Process Control .................. 13
5 Procedures to carry out prior to Cultivation .................... 14
5.1 Preparing the Reagent Bottles and Hoses .................. 15
5.1.1 Connecting a Pump with a Reagent Bottle... 15
5.1.2 Equipping a Reagent Bottle for Connection
with a Fluid Inlet Line .................................. 17
5.2 Calibrating the pH Sensor .......................................... 18
5.3 Sterilising the Reagent Bottles and Sensors before
Cultivation ................................................................. 18
5.4 Equipping the Gassing Line on the Equipment Side ... 20
5.5 Connecting the Reagent Bottles, Fitting the Sensors .. 20
5.6 Connecting the Sensors ............................................. 20
5.7 Fitting and Connecting the Heater Pad ....................... 21
5.8 Connecting the Motor................................................. 23
5.9 Connecting the Gassing ............................................. 24
5.10 Calibrating the pO
6 Cultivation .......................................................................... 26
6.1 Safety ........................................................................ 26
7 Procedures to carry out after Cultivation ......................... 27
7.1 Emptying the Fluid Inlet Lines .................................... 27
7.2 Disconnecting Cable and Hose Connections,
Removing the Sensors ............................................... 28
Labfors 5 Cell - Single-Use Vessel Option

Table of Contents

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