Zeiss Axiovert 200 Operating Manual page 99

Axiovert 200
The specimen field with the relevant pupil image
of the VAREL diaphragm is shown in Fig. 3-22. In
the pupil image, the diaphragm appears rotated by
180°.
Shifting the VAREL illumination to outside the pupil corresponds to unilateral darkfield
illumination.
Shifting the VAREL illumination between the Ph and VAREL rings of the objective
corresponds to oblique brightfield illumination.
Objective
Ph ring VAREL ring
Fig. 3-23 Pupil images in VAREL contrast
B 40-080 e 03/01
OPERATION
Illumination and contrasting techniques
Var contrast
VAREL diaphragm
Fig. 3-22 VAREL contrast with microtiter
plates
Oblique brightfield
illumination
Carl Zeiss
Unilateral darkfield
illumination
3-39