Zeiss Axioskop 2 Manual
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Zeiss Axioskop 2 Manual

Routine microscope
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Axioskop 2
Routine Microscope
Operating Manual

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Summary of Contents for Zeiss Axioskop 2

  • Page 1 Axioskop 2 Routine Microscope Operating Manual...

  • Page 2: Copyright

    INTRODUCTION Copyright Axioskop 2 / Axioskop 2 Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument.

  • Page 3: Table Of Contents

    CHAPTER 1 INSTRUMENT DESCRIPTION ..................1-2 Name and intended application ...................1-2 Instrument description and main features ..............1-2 Axioskop 2 (manual) microscope configurations and modules ........1-4 Axioskop 2 microscope configurations and modules ..........1-8 Objectives ......................... 1-12 Eyepieces ........................1-14 Stage micrometers and eyepiece reticles ..............
  • Page 4 Changing the binocular tube..................2-25 2.2.10 Attachment of intermediate tube with height adjustment ......... 2-26 2.2.11 Attachment of adapter for the Axioskop 2 ..............2-27 2.2.12 Changing the technique modules on the reflector turret ........... 2-28 2.2.13 Changing the filter set in the FL reflector module ............2-28 2.2.14...
  • Page 5 CHAPTER 3 OPERATION....................... 3-3 Overview of operation and function controls ...............3-3 3.1.1 Operation and function controls of the Axioskop 2, manual ......... 3-4 3.1.2 Overview of operation and function controls Axioskop 2 ........3-10 Switching on and basic settings ................. 3-18 3.2.1...
  • Page 6 INTRODUCTION Contents Axioskop 2 / Axioskop 2 Page CHAPTER 4 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE........4-3 Instrument care ......................4-3 Instrument maintenance .................... 4-4 4.2.1 Performing checks ...................... 4-4 4.2.2 Changing the fuses..................... 4-4 4.2.3 Check instrument voltage and change it, if required ............ 4-5 Troubleshooting ......................

  • Page 7: Microscopy In A Few Steps

    Microscopy in a few steps using the example of "Setting of transmitted-light brightfield according to KÖHLER" Before starting to use the Axioskop 2 and/or Axioskop 2 microscopes, make sure to read the notes on instrument safety and the Description (Chapter 1) and Start-up (Chapter 2) chapters.

  • Page 8: Notes On Instrument Safety

    Notes on instrument safety Axioskop 2 / Axioskop 2 Notes on instrument safety The Axioskop 2 and Axioskop 2 microscopes were designed, produced and tested in compliance with DIN 61010-1 (IEC 1010-1), Safety requirements for electrical measuring, control and laboratory instruments, and meet the requirements of appendix I of directive 73/23/EC and the relevant CSA and UL directives.
  • Page 9 If it is established that the protection measures are no longer effective, the instrument must be switched off and safeguarded against inadvertent operation. Contact a Zeiss service agency or Carl Zeiss microscopy service for the repair of the instrument.
  • Page 10 The instruments must be operated by trained personnel only who must be aware of the possible danger involved with microscopy and the relevant application. The Axioskop 2 / Axioskop 2 is an optical precision instrument which can be impaired in its performance or damaged when handled improperly.

  • Page 11: Notes On Warranty

    This forfeits all the claims against warranty. With the exception of the work specified in this manual, no maintenance or repair of the Axioskop 2 / Axioskop 2 may be performed.

  • Page 12: Overall View Of Axioskop 2

    INTRODUCTION Overall view of the Axioskop 2 Axioskop 2 / Axioskop 2 Overall view of the Axioskop 2 Eyepieces Binocular tube Stand Incident-light illuminator Transmitted-light illuminator Condenser Mechanical stage Objectives 0-12 B 40-075 e 06/98...

  • Page 13: Overall View Of Axioskop 2

    INTRODUCTION Axioskop 2 / Axioskop 2 Overall view of the Axioskop 2 Overall view of the Axioskop 2 Eyepieces Binocular ergotube Stand Incident-light illuminator FL excitation filter wheel Transmitted-light illuminator Condenser Mechanical stage Objectives B 40-075 e 06/98 0-13...
  • Page 14 INTRODUCTION Axioskop 2 / Axioskop 2 0-14 B 40-075 e 06/98...
  • Page 15 INSTRUMENT DESCRIPTION Contents INSTRUMENT DESCRIPTION ..................1-2 Name and intended application..................1-2 Instrument description and main features.................1-2 Axioskop 2 (manual) microscope configurations and modules...........1-4 Axioskop 2 microscope configurations and modules ..........1-8 Objectives........................1-12 Eyepieces........................1-14 Stage micrometers and eyepiece reticles................. 1-14 Technical Data .......................

  • Page 16: Mot

    Axioskop 2, such as tubes, intermediate tubes and condensers, are easy to attach. The Axioskop 2 is offered as a manual and a motorized / coded version. The basic stand is equipped with either manual (Axioskop 2) or motorized / coded operation and function controls (Axioskop 2 ), but is otherwise identical for both versions.
  • Page 17 − Multi-observation equipment for 2, 9 or more viewing stations − Drawing device with intermediate tube for image projection has the following features which In addition to the above instrument features, the Axioskop 2 differ from the manual version: − High-precision step motor drive for stage focusing −...

  • Page 18: Axioskop 2 (Manual) Microscope Configurations And Modules

    INSTRUMENT DESCRIPTION Axioskop 2 microscope configurations ... Axioskop 2 / Axioskop 2 Axioskop 2 (manual) microscope configurations and modules Fig. 1-1 Axioskop 2 (manual) microscope configurations B 40-075 e 06/98...
  • Page 19 INSTRUMENT DESCRIPTION Axioskop 2 / Axioskop 2 Axioskop 2 microscope configurations ... Key to Fig. 1-1 Stand Eyepiece Binocular tube 30°/23 HBO 100 W transformer HBO 103 illuminator HAL 12 V 100 W halogen lamp FL filter slider Objective DIC slider Compensator λ...

  • Page 20: Axioskop 2 Mot Microscope Configurations And Modules

    Axioskop 2 / Axioskop 2 Axioskop 2 (manual) microscope configurations) Cat. No. Configurations The "Axioskop 2" routine microscope is offered in the following configurations: for transmitted-light brightfield with objectives "A-Plan" 5x, 10x, 40x and 100x for transmitted-light brightfield with objectives "Achroplan" 4x, 10x, 40x and 100x for transmitted-light brightfield and phase contrast with objectives "Achroplan"...
  • Page 21 452376 Intermediate tube for image projection from the Axioplan 2 line 452181-9901 Adapter for Axioskop 2 to allow the use of tubes / intermediate tubes from the Axioplan 2 line 452969 MC 80 microscope camera for 24 mm x 36 mm...

  • Page 22: Axioskop 2 Mot Microscope Configurations And Modules

    INSTRUMENT DESCRIPTION Axioskop 2 microscope configurations ... Axioskop 2 / Axioskop 2 Axioskop 2 microscope configurations and modules Fig. 1-2 Axioskop 2 microscope configurations B 40-075 e 06/98...
  • Page 23 INSTRUMENT DESCRIPTION Axioskop 2 / Axioskop 2 Axioskop 2 microscope configurations ... Key to Fig. 1-2: Stand Eyepiece Binocular ergotube 6-25°/23 HBO 100 W transformer HBO 103 illuminator HAL 12 V 100 W halogen illuminator FL filter slider Objective DIC slider Compensator λ...
  • Page 24 INSTRUMENT DESCRIPTION Axioskop 2 microscope configurations ... Axioskop 2 / Axioskop 2 Axioskop 2 microscope configurations Cat. No. Configurations The "Axioskop 2 " routine microscope is offered in the following configurations: for transmitted-light brightfield with Achroplan objectives 4x, 10x, 40x and 100x for transmitted-light brightfield and phase contrast with Plan-Neofluar objectives 2.5x, 10x Ph 1, 20x Ph 2, 40x Ph 2 and 100x Ph 3...
  • Page 25 452376 Intermediate tube for image projection from the Axioplan 2 line 452181-9901 Adapter for Axioskop 2 to allow the use of tubes / intermediate tubes from the Axioplan 2 line 452969 MC 80 microscope camera for 24 mm x 36 mm...

  • Page 26: Objectives

    INSTRUMENT DESCRIPTION Objectives Axioskop 2 / Axioskop 2 Objectives The objectives are the optical centerpiece of the microscope. The following is an example of how objectives can be labelled: Achroplan 10x/0.25 ∞ 0.17 where 10x : objective magnification, with a defined...

  • Page 27: Objectives

    (do not forget to unlock them again!). The following objectives are ideal for the Axioskop 2 and Axioskop 2 microscopes: Microscopy technique...

  • Page 28: Eyepieces

    INSTRUMENT DESCRIPTION Eyepieces / Stage micrometers and ... Axioskop 2 / Axioskop 2 Eyepieces The following eyepieces are offered for the Axioskop 2 and Axioskop 2 Eyepiece Image angle Application Cat. No. Eyepiece W-PL 10x/23 Br. foc. (2x) 49.4° with all tubes, see pp. 1-6, 1-10 455043, aspheric Eyepiece W-PL 10x/23 Br.
  • Page 29 INSTRUMENT DESCRIPTION Axioskop 2 / Axioskop 2 Stage micrometers and ... Illustration Description, Technical Data Cat. No. Eyepiece reticle / d = 26 mm 474064 Crossline micrometer 10:100, d = 26 mm 474066-9901 gradation length = 10 mm increments = 0.1mm gradation tolerance ≤...

  • Page 30: Technical Data

    HAL 100 ..approx. 280 mm x 670 mm x 500 mm Axioskop 2/Axioskop 2 with ergo-phototube and HBO 103 ..approx. 280 mm x 800 mm x 500 mm Weight Axioskop 2 ........................approx. 18.4 kg Axioskop 2 ......................approx. 22.4 kg Ambient conditions Storage and transport (in packaging) Permissible ambient temperature ..................
  • Page 31 Line frequency ........................50 to 60 Hz Power consumption when HBO 103 is used..................265 VA Fuses in accordance with IEC 127 Axioskop 2 microscope stand ..................T 4 A/H, 5x20 mm ................T 4 A/H, 5x20 mm Axioskop 2 microscope stand HBO 100 W transformer ..................
  • Page 32 INSTRUMENT DESCRIPTION Technical data Axioskop 2 / Axioskop 2 Axioskop 2: Stand with manual stage focusing coarse drive ......................6 mm / rotation Fine drive ................0.1 mm / rotation; 1 µm increments lifting range ....................... max. 25 mm height stop ....................can be set mechanically Achromatic-aplanatic universal condenser 0.9 with...

  • Page 33: Start-Up

    Changing the binocular tube ..................2-25 2.2.10 Attachment of intermediate tube with height adjustment..........2-26 2.2.11 Attachment of adapter for the Axioskop 2 ..............2-27 2.2.12 Changing the technique modules on the reflector turret..........2-28 2.2.13 Changing the filter set in the FL reflector module ............2-28 2.2.14...
  • Page 34 Changing the binocular tube......................2-25 Fig. 2-40 Attachment of intermediate tube with height adjustment ............... 2-26 Fig. 2-41 Attachment of adapter for the Axioskop 2 ..................2-27 Fig. 2-42 Changing of technique modules...................... 2-28 Fig. 2-43 Changing the filter set in the FL reflector module ................2-28 Fig.

  • Page 35: Initial Start-Up

    The Axioskop 2 / Axioskop 2 can be installed, converted and started up by the customer himself. However, it is also possible to have the microscope installed or converted by Zeiss service staff against an extra charge. Before installation and start-up of the microscope, make sure to carefully read the Notes on instrument safety.

  • Page 36: Attachment Of Binocular Tube Or Phototube

    All the binocular tubes included in the microscope configurations listed in section 1.3 or 1.4 can be attached to the Axioskop 2 / Axioskop 2 described below. • Loosen hexagonal screw (2-2/3) using the SW3 ball-headed screwdriver. Remove dust covers (2-2/2) from the tube underside and the dovetail mount on the stand.
  • Page 37 START-UP Axioskop 2 / Axioskop 2 Initial start-up Inserting the eyepiece reticle The eyepieces PL 10x/23 Br. foc. are intended for use with eyepiece reticles. The slight image shift caused by the additional path through the glass is taken into account on...

  • Page 38: Setting Of The Interpupillary Distance Of The Binocular Tube

    • Remove dust caps (2-6/2) from the respective openings in the nosepiece. • Remove objectives (2-6/3) from case and screw them in nosepiece (2-6/1) clockwise one by one, starting with the lowest magnification. In the case of the Axioskop 2 , start with objective position coded nosepiece.

  • Page 39: Attachment Of Universal Condenser

    START-UP Axioskop 2 / Axioskop 2 Initial start-up 2.1.7 Attachment of universal condenser • Move stage carrier to the upper stop position via the focusing drive. • Use lever (2-7/8) to fold out the front lens on the condenser or, in the case of the motorized version, swing it out by pressing the respective key.

  • Page 40: Connection To The Line

    START-UP Initial start-up Axioskop 2 / Axioskop 2 2.1.8 Connection to the line • Connect microscope socket (2-9/2) to the line socket via a line cable. The microscope can be connected to line voltage between 100 and 240 VAC, 60 - 60 Hz. The power unit sets itself automatically to the available line voltage within this range.

  • Page 41: Hal 100 Halogen Illuminator

    START-UP Axioskop 2 / Axioskop 2 Initial start-up 2.1.10 HAL 100 halogen illuminator Attachment HAL 100 halogen illuminator • Remove cover from transmitted-light tube (2-12/3). • Insert dovetail (2-13/2) of lamp housing (2-12/1) in transmitted-light tube (2-12/3) on the instrument rear and use the SW 3 ball-headed screwdriver to tighten clamping screw (2-12/2).

  • Page 42: Hbo 103 Illuminator

    START-UP Initial start-up Axioskop 2 / Axioskop 2 Fine adjustment • Attach illuminator to the stand again and tighten clamping screw (2-12/2). • Remove diffusion disk (3-1/15) and filters (3-1/18), if switched in, from the beam path. • Focus on the specimen using objective ≤ 40x and search for a free object area.
  • Page 43 START-UP Axioskop 2 / Axioskop 2 Initial start-up • Insert multi-pin plug of the HBO 103 illuminator (2-14/3) into socket (2-15/2) of the HBO 100 W transformer and secure it with captive coupling ring. • Connect line cable to socket (2-15/1) of the HBO 100 W transformer first, then connect it to the line.
  • Page 44 START-UP Initial start-up Axioskop 2 / Axioskop 2 Fig. 2-18 Focal spots of HBO 103 before Fig. 2-19 Focal spots of HBO 103 after coarse coarse adjustment adjustment During coarse adjustment of the focal spots, adjusting screws (2-17/2 and 5) marked in red must not be changed, since they influence the reflector setting in the HBO 103 illuminator.
  • Page 45 START-UP Axioskop 2 / Axioskop 2 Initial start-up • If required, set knurled knob (2-17/6) and adjusting screw (2-17/4) alternately until both focal spots have the same dimension (see Fig. 2-19). • Use adjusting screws (2-17/1 and 3) to adjust the brighter focal spot in the adjusting circle, e.g. on the left side, in the same way as the focal spot image (2-20).

  • Page 46: Connect Pc / Laptop To The Axioskop 2

    START-UP Initial start-up Axioskop 2 / Axioskop 2 2.1.12 Connect PC / Laptop to the Axioskop 2 microscope Connect the PC to the Axioskop 2 microscope via the RS-232 serial interface (2-22/2) on the instrument rear. Other interfaces on the instrument rear are designed as SUB-BUS (2-22/3) and combined CAN/SUB-BUS (2-22/1) connectors.

  • Page 47: Attachments And Conversions

    START-UP Axioskop 2 / Axioskop 2 Attachments and conversions Attachments and conversions The steps required for attachments to and conversions of the microscope are explained in the following. After this, the respective modules must be reset in a functioning status again.

  • Page 48: Changing The Phase Stop For Phase Contrast Or Darkfield In The Universal Condenser

    START-UP Attachments and conversions Axioskop 2 / Axioskop 2 • In the case of the motorized condenser (Axioskop 2 ), plug connecting cable in the socket on the left front of the stand (2-24/1) and tighten screwing ring. • The achromatic-aplanatic condenser 1.4 H DIC and other condensers from the Axioplan 2 line are also suitable for attachment.

  • Page 49: Changing The Dic Prism In The Universal Condenser

    START-UP Axioskop 2 / Axioskop 2 Attachments and conversions 2.2.3 Changing the DIC prism in the universal condenser • To loosen the cover on the condenser underside (2-26/1), loosen both grub screws using the SW 2 screwdriver remove cover. Position the turret disk containing the DIC prism to be exchanged in the exchange opening and hold it on the knurled ring.

  • Page 50: Attachment Of Filter Mount

    START-UP Attachments and conversions Axioskop 2 / Axioskop 2 2.2.4 Attachment of filter mount • Hold filter mount (2-27/5) parallel to the underside of the condenser carrier (2-27/3) and screw holding pin (2-27/4) of the filter mount (2-27/5) into the front threaded hole on the left below the condenser carrier (2-27/3) until stop using the SW 2 angled Allen key 2-27/6).

  • Page 51: Changing The Hal 100 Halogen Lamp

    START-UP Axioskop 2 / Axioskop 2 Attachments and conversions 2.2.6 Changing the HAL 100 halogen lamp • Switch off the microscope as described in section 2.1.9, remove plug (2-12/5) from 12 V / 100 W socket (2-12/4) allow instrument to cool down for approx. 15 mins.
  • Page 52 START-UP Attachments and conversions Axioskop 2 / Axioskop 2 After expiry of the average operation time of 300 h, the HBO 103 W/2 mercury vapor short-arc lamp must be exchanged. The operating time of the lamp can be checked on...

  • Page 53: Changing The Hbo 103 W/2 Mercury Vapor Short-Arc Lamp

    START-UP Axioskop 2 / Axioskop 2 Attachments and conversions • Exchange the contact wire (2-32/13) if it is strongly oxidized. For this purpose, loosen clamping screw (2-32/14) using SW 1 mm Allen key. • Insert new HBO 103 W/2 lamp in the cooling body with the smaller shaft diameter and tighten clamping screw (2-32/4).

  • Page 54: Changing The Mechanical Stage

    START-UP Attachments and conversions Axioskop 2 / Axioskop 2 2.2.8 Changing the mechanical stage Fixed mechanical stage 75x50 R Removing the stage • Unscrew 4 fixation screws (2-33/5) on the stage carrier (2-33/6) using angled SW 3 Allen key (2- 33/4) •...
  • Page 55 START-UP Axioskop 2 / Axioskop 2 Attachments and conversions Centering the stage When objectives with a high magnification are used, centering can be exact for one selected objective only. All stages are factory-precentered, i.e. a set specimen detail remains in the image center even when the stage is rotated.
  • Page 56 START-UP Attachments and conversions Axioskop 2 / Axioskop 2 Setting the smoothness (torque) of the drive for stage adjustment The torque for x and y movement can be individually matched for both mechanical stages. They are factory-aligned to a medium value.

  • Page 57: Changing The Binocular Tube

    START-UP Axioskop 2 / Axioskop 2 Attachments and conversions Changing the specimen holder • Loosen two countersunk screws (2-38/1) using SW 2 Allen key (2-38/3). • Remove specimen holder (2-38/2) from the mechanical stage (2-38/4) in an upward direction. • Attach required specimen holder (2-38/5) to the mechanical stage and tighten two countersunk screws.

  • Page 58: Attachment Of Intermediate Tube With Height Adjustment

    START-UP Attachments and conversions Axioskop 2 / Axioskop 2 2.2.10 Attachment of intermediate tube with height adjustment The intermediate tube with height adjustment may only be used in combination with a binocular ergotube. When removing the intermediate tube and attaching the binocular ergotube, make absolutely sure to screw the tube lens into the binocular ergotube again.

  • Page 59: Attachment Of Adapter For The Axioskop 2

    2.2.11 Attachment of adapter for the Axioskop 2 The adapter for the Axioskop 2 is used to attach intermediate tubes and/or tubes of the Axioplan 2 to the Axioskop 2 / Axioskop 2 • Use SW 3 ball-headed screwdriver to loosen clamping screw (2-41/5) and remove available tube of the Axioskop 2 in an upward direction.

  • Page 60: Changing The Technique Modules On The Reflector Turret

    START-UP Attachments and conversions Axioskop 2 / Axioskop 2 2.2.12 Changing the technique modules on the reflector turret • Remove cover (2-42/1) on the reflector turret by pulling it forward. • Loosen two fixation screws (2-42/3) using SW 2.5 ball-headed screwdriver and remove technique module (2-42/2) by pulling forward.

  • Page 61: Changing The Beam Splitter In The Fl Reflector Module

    START-UP Axioskop 2 / Axioskop 2 Attachments and conversions 2.2.14 Changing the beam splitter in the FL reflector module Attachment of filters and of the beam splitter requires utmost care to prevent damage and contamination of the optical components. We would recommend you to order completely equipped FL reflector modules, since changing the beam splitter requires much skill.

  • Page 62: Attachment Of Active Coobservation Equipment For 2 Users

    2.2.15 Attachment of active coobservation equipment for 2 users The active coobservation equipment for 2 users can only be attached to a Axioskop 2 / Axioskop 2 microscope stand if the incident-light illuminator (e.g. HBO 103) has been removed. • Use SW 3 ball-headed screwdriver to loosen clamping screw (2-45/12) and remove available tube in an upward direction.
  • Page 63 START-UP Axioskop 2 / Axioskop 2 Attachments and conversions Binocular ergotube Binocular tube Dust cap Rear dovetail Socket for power supply Heat protection shield Fixation screw Clamping screw Support point (stand) Power unit Plug Clamping screw Dovetail (stand) Dust cap...

  • Page 64: Attachment Of Multi-Observation Equipment

    We would recommend you to have assembly of the multi-observation equipment performed by Zeiss service staff (also see 4.5), since careful optical adjustment and centering of the fields of view of the various observation tubes is required. Should you want to perform assembly yourself, please proceed as follows: •...
  • Page 65 START-UP Axioskop 2 / Axioskop 2 Attachments and conversions The binocular tubes of the multi-observation equipment can be turned and fixed in position as required by the user. The multi-observation equipment with light pointer permits attachment of 2, 5, 9 and more observation devices.

  • Page 66: Attachment Of Intermediate Tube For Image Projection

    • Use SW 3 ball-headed screwdriver to loosen clamping screw (2-47/8) and remove available tube of the Axioskop 2 in an upward direction. • Insert dovetail of the adapter for the Axioskop 2 (2-47/6) in the stand opening (2-47/9) on the underside and slightly tighten clamping screw (2-47/8).

  • Page 67: Operation

    Overview of operation and function controls ..............3-3 3.1.1 Operation and function controls of the Axioskop 2, manual..........3-4 3.1.2 Overview of operation and function controls of the Axioskop 2 ....... 3-10 Switching on and basic settings ..................3-18 3.2.1 Axioskop 2, manual ....................... 3-18 3.2.2...
  • Page 68 List of illustrations Axioskop 2 / Axioskop 2 List of illustrations Fig. 3-1 Operation and function controls of the Axioskop 2, manual ..........3-4 Fig. 3-2 Universal condenser ......................3-8 Fig. 3-3 Nosepiece with eyes for compensators and DIC slider............3-8 Fig.

  • Page 69: Overview Of Operation And Function Controls

    OPERATION Overview of operation and function controls The Axioskop 2 routine microscopes are offered in a manual version and in a version with motorized/coded components. In the "Operation" chapter, the manual setting and operation functions are explained first, and the motorized / coded functions are explained afterwards, where necessary.

  • Page 70: Operation And Function Controls Of The Axioskop 2, Manual

    OPERATION Operation and function controls Axioskop 2 / Axioskop 2 3.1.1 Operation and function controls of the Axioskop 2, manual Fig. 3-1 Operation and function controls of the Axioskop 2, manual B 40-075 e 06/98...
  • Page 71 OPERATION Axioskop 2 / Axioskop 2 Operation and function controls Key to Fig. 3-1: Eyepieces Binocular tube Centering screws for luminous-field diaphragm (incident light) Pushrod for luminous-field diaphragm (incident light) Compartment for filter slider Centering screws for aperture diaphragm (incident light)
  • Page 72 OPERATION Operation and function controls Axioskop 2 / Axioskop 2 Eyepieces − Both eyepiece versions, E-PL 10x/23 Br. foc. and W-PL 10x/23 Br. foc., enable compensation of ametropia of the user's eyes (see section 2.1.3) and also accept eyepiece reticles (see section 1.7).
  • Page 73 OPERATION Axioskop 2 / Axioskop 2 Operation and function controls Color temperature key 3200 K − When the color temperature key 3200 K is activated, LED step 9 lights up. The DC voltage 10.5 V is then available for the halogen lamp, which corresponds to the color temperature of 3200 K. This is required for photomicrography using color films (artificial light).
  • Page 74 OPERATION Operation and function controls Axioskop 2 / Axioskop 2 Universal condenser Depending on the version, the achromatic- aplanatic universal condenser (3-2/1) is equipped as follows: − fold-out front lens. − turret disk for: brightfield - without or with DIC I, II, III...
  • Page 75 OPERATION Axioskop 2 / Axioskop 2 Operation and function controls Reflector turret − Accepts the maximum of four technique modules, such as the FL reflector module and the shutter module A for incident light, and the Optovar module and analyzer module for transmitted light.

  • Page 76: Overview Of Operation And Function Controls Axioskop

    OPERATION Operation and function controls Axioskop 2 / Axioskop 2 3.1.2 Overview of operation and function controls of the Axioskop 2 Fig. 3-5 Operation and function controls of the Axioskop 2 3-10 B 40-075 e 06/98...
  • Page 77 OPERATION Axioskop 2 / Axioskop 2 Operation and function controls Key to Fig. 3-5: Motorized, 8-position FL excitation filter wheel for filter diameter 25 mm in epi-fluorescence (optional) LED lamp voltage array Pilot lamp Control for light intensity Motorized focusing drive (on both sides) for coarse / fine adjustment...
  • Page 78 OPERATION Operation and function controls Axioskop 2 / Axioskop 2 Motorized universal condenser Depending on the version, the achromatic-aplanatic universal condenser is equipped as follows: − Motorized fold-out front lens. − Motorized turret disk with brightfield insert, darkfield stop and phase stops (centering), DIC prisms combined with iris stop.
  • Page 79 When the stage is in the low position, the motorized focusing drive is switched off. To avoid squashing of your fingers during fast stage lowering (changing key), the Axioskop 2 has been equipped with a safety switch-off device. The switch-off point (3-7/3) is approx. 11 mm below the upper stop (3-7/1) of the entire travel range of 25 mm.
  • Page 80 OPERATION Operation and function controls Axioskop 2 / Axioskop 2 for fast stage lifting − When this key is activated, the stage is moved back to the focus position last stored. In the case of different specimen slide thickness, there is a risk of the specimen and/or the objective being damaged during fast stage lifting.
  • Page 81 OPERATION Axioskop 2 / Axioskop 2 Operation and function controls Binocular ergo-phototube 6-25°/23 (100/100) The camera port (3-9/1) of the binocular ergo- phototube allows the attachment of SLR-cameras, microscope cameras and video cameras via the relevant adapters. The light can be directed either to the eyepieces or to the attached camera via a pushrod.
  • Page 82 OPERATION Operation and function controls Axioskop 2 / Axioskop 2 Filter mount The filter mount allows three additional dia. 32mm color filters to be swung in the beam path together or separately. • Insert filter in one of the three filter plates (3-11/1).
  • Page 83 Intermediate tube with height adjustment Attachment of the intermediate tube with height adjustment allows the viewing height of the Axioskop 2 to be increased continuously from 60 to 110 mm. This intermediate tube can only be used in combination with the binocular ergotube 6-25°/23...

  • Page 84: Switching On And Basic Settings

    − In the manual mode, the function of the light manager is switched off. Illumination intensity and condenser settings must be made manually, as in the Axioskop 2. Programming mode: − In the programming mode, the user can individually match and store the focusing speeds and focus positions of the various objectives (parfocality).
  • Page 85 OPERATION Axioskop 2 / Axioskop 2 Switching on and basic settings Overview of function and programming modes: Function modes Programming mode automatic manual • switch on Axioskop 2 • Keep key "transmitted light • Keep Set key (3-14/1) on Start with the on / off switch on / off"...
  • Page 86 Axioskop 2 / Axioskop 2 Illumination setting in the automatic mode (light manager function) When switched on, the Axioskop 2 always starts in the automatic mode. The factory-aligned (average) values or the last stored settings of illumination intensity, condenser functions, transmitted...
  • Page 87 Illumination intensity and condenser functions must then be operated manually, as in the Axioskop 2. The manual mode is set as follows: • Switch off the microscope with the on / off switch (3-1/27).
  • Page 88 OPERATION Switching on and basic settings Axioskop 2 / Axioskop 2 The parameters for stage focusing are set as follows: • Swing in the dry objective with the highest magnification factor via the nosepiece. • Set reflector turret to transmitted-light position (see 3-1/23).
  • Page 89 OPERATION Axioskop 2 / Axioskop 2 Switching on and basic settings • Leave the programming mode by shortly pressing the Set key. The end of the programming mode is confirmed by a beep when the Set key is released. The pilot lamp lights up in green again.

  • Page 90: Illumination And Contrasting Techniques

    Transmitted-light brightfield settings according to KÖHLER on the manual Axioskop 2 − The manual Axioskop 2 has been set up as described in chapter 2. − The manual Axioskop 2 has been switched on. • Set the image brightness via voltage control (3-16/1) on the microscope stand.
  • Page 91 OPERATION Axioskop 2 / Axioskop 2 Illumination and contrasting techniques • Swing in 10x objective (yellow ring, see p. 1-12) in the nosepiece (3-16/6) and focus on the specimen via drive (3-16/2). • Close luminous-field diaphragm (3-16/4) until it becomes visible (even if not in focus) in the field of view (3-16/A).
  • Page 92 • Setting of KÖHLER illumination to be performed in the same way as described in section 3.3.1 (3) for the manual Axioskop 2. Unlike this procedure, however, the luminous-field diaphragm must be fully opened after the correct setting of the condenser. Shortly press the Set key on the instrument rear after the last setting step to store the settings.

  • Page 93: Setting Of Transmitted-Light Darkfield

    • Setting of KÖHLER illumination is identical to transmitted-light brightfield, except that the highest aperture objective must be used instead of the 10x objective. • Turn turret disk of the universal condenser to position D (for Axioskop 2 using key (3-6/4 or 5)) and swing in condenser front lens (for Axioskop 2 using key (3-6/3)).
  • Page 94 OPERATION Illumination and contrasting techniques Axioskop 2 / Axioskop 2 • Insert eyepiece in the tube again. • If the height of the dark field condenser has been set correctly and sensibly enough, any visible brightening in the field of view can be reduced, and the luminous-field diaphragm image will be almost perfectly in focus.

  • Page 95: Setting Of Transmitted-Light Phase Contrast

    Configurations of the manual Axioskop 2 and the Axioskop 2 − Phase contrast objectives with phase rings Ph 1, Ph 2 or Ph 3 for different average numeric apertures which can also be used in brightfield without any restriction.
  • Page 96 OPERATION Illumination and contrasting techniques Axioskop 2 / Axioskop 2 Transmitted-light phase contrast settings on manual Axioskop 2 and Axioskop 2 • Swing phase contrast objective, e.g. Ph 1, in the beam path. • Insert phase stop labelled like the phase contrast objective, e.g.

  • Page 97: Setting Of Transmitted-Light Polarization

    (mostly with biological objects) to white, yellow, red and blue occur as a function of birefringence, thickness and orientation of the object. These interference colors can be of the 1 or a higher order. Axioskop 2 (manual) and Axioskop 2 configuration − Objectives suitable for polarization − Rotary mechanical stage 75x50/240° R −...
  • Page 98 − Analyzer module D in reflector turret − Adjusting specimen for polarization microscopy (453679) Setting of Axioskop 2 (manual) and Axioskop 2 • Set the microscope as for transmitted-light brightfield (see section 3.3.1 (3)), taking care to ensure that the interpupillary distance of the binocular tube has been correctly set (see section 2.1.3 (2)).
  • Page 99 OPERATION Axioskop 2 / Axioskop 2 Illumination and contrasting techniques • Turn the mechanical stage with specimen, e.g. an artificial fiber until the specimen displays maximum darkness. The fiber is now parallel to one of the two directions of the crosslines. If pronounced deviations (5°...
  • Page 100 OPERATION Illumination and contrasting techniques Axioskop 2 / Axioskop 2 Conclusions The grayish white color first occurring in the bright position in the above example corresponds to a path difference of 150 nm, according to the Michel Lévy color chart (3-23).
  • Page 101 Unlike polarization contrast, circular polarization contrast does not feature any dark positions which depend on the turning angle (azimuth) of the specimen in relation to the polarizer or analyzer. All transparent specimens feature their specific interference colors in optical anisotropy. Axioskop 2 (manual) and Axioskop 2 configuration − Objectives suitable for polarization −...

  • Page 102: Setting Of Transmitted-Light Differential Interference Contrast (Dic)

    − DIC analyzer module in reflector turret − Rotary mechanical stage should be preferred Setting of transmitted-light DIC on Axioskop 2 (manual) and Axioskop 2 • Swing in the DIC-suitable objective via the nosepiece (3-25/1). Push the relevant DIC slider (3-25/3) in the compartment on the nosepiece until it click-stops.
  • Page 103 OPERATION Axioskop 2 / Axioskop 2 Illumination and contrasting techniques • Bring SENARMONT polarizer (3-25/7), consisting of carrier with λ/4 plate (above) and rotary polarizer (below), in click-stop position below the condenser, unless polarization filters are integrated. • Set luminous-field diaphragm and aperture diaphragm (3-25/6) in accordance with the KÖHLER rules.
  • Page 104 Illumination and contrasting techniques Axioskop 2 / Axioskop 2 Objective in nosepiece Reflector turret DIC slider Knurled screw λ-compensator Sliding knob for aperture diaphragm SENARMONT polarizer Fig. 3-25 Components for the transmitted-light DIC technique on the Axioskop 2 3-38 B 40-075 e 06/98...

  • Page 105: Setting Of Epi-Fluorescence

    2.1.11 by using the adjusting aid. If required, re-adjustment must be performed depending on the operation time. Setting of epi-fluorescence on the Axioskop 2 (manual) and Axioskop 2 The first epi-fluorescence setting is considerably facilitated if the Plan-Neofluar objective 20x/0.50 and a specimen featuring pronounced fluorescence is used.
  • Page 106 OPERATION Illumination and contrasting techniques Axioskop 2 / Axioskop 2 • Switch on the HBO 103 or HBO 50 mercury vapor short-arc lamp (3-26/2) and allow it to heat up to its operation temperature for approx. 15 mins. • Select the FL reflector module in reflector turret...

  • Page 107: Documentation

    Documentation 3.4.1 Attachment of photomicrography equipment The Axioskop 2 / Axioskop 2 equipped with a binocular phototube (3-27/5) can be switched from observation to photomicrography (pushrod pulled out) using the pushrod (3-27/7). The special T2 adapter 2.5x and other T2 adapters permit commercially available 35 mm SLR cameras (SLR - Single Lens Reflex) and special microscope cameras (e.g.
  • Page 108 SLR cameras, but also of microscope cameras, e.g. MC 80 or MC 200 from Carl Zeiss. You can order the MC 80 CHIP MC 200 microscope cameras under the catalog numbers given in section 1.3, "microscope CHIP configurations".
  • Page 109 416009 T2 Adapter for PENTAX (KA-bayonet) 416011 If a PC or notebook with installed software is already connected to the Axioskop 2 , the microscope camera can be controlled directly via the user interface of the software. For this purpose,...

  • Page 110: Attachment Of Videomicroscopy Equipment

    3.4.2 Attachment of videomicroscopy equipment Due to ICS optics from Carl Zeiss, the completely corrected intermediate image falls directly on the target of the video camera. This permits optimum quality of the image transfer, even if high-resolution video cameras are used.
  • Page 111 Axioskop 2 / Axioskop 2 Documentation The 60 - 44 adapter also allows video adapters with 44 mm interface to be used with the phototube of the Axioskop 2 / Axioskop 2 with 60 mm interface. Video adapter Suitable for: Comments (Cat.

  • Page 112: Multi-Observation Equipment

    OPERATION Multi-observation equipment Axioskop 2 / Axioskop 2 Multi-observation equipment 3.5.1 Attachment of active coobservation equipment for 2 users The active coobservation equipment is intended for two users and consists of the following: − Basic body (3-30/3) with two mounts for the binocular tubes −...
  • Page 113 OPERATION Axioskop 2 / Axioskop 2 Multi-observation equipment Binocular ergotube Binocular tube Basic body Power unit Knob for brightness control Lever for light pointer Fig. 3-30 Active coobservation equipment for 2 users B 40-075 e 06/98 3-47...

  • Page 114: Attachment Of Multi-Observation Equipment

    − Binocular tubes 30°/23 for further viewing stations (3-31/6 and 7, 452927) We would recommend you to have the multi-observation equipment assembled by Carl Zeiss service staff, since careful optical adjustment and centering of the fields of view of the various observation tubes is required.
  • Page 115 OPERATION Axioskop 2 / Axioskop 2 Multi-observation equipment Knob for brightness control Coobservation tube Basic body End connector L Lever for light pointer Power unit Binocular tube End connector R Connecting piece L Connecting piece R Coobservation tube Fig. 3-31...

  • Page 116: Drawing Device

    OPERATION Drawing device Axioskop 2 / Axioskop 2 Drawing device 3.6.1 Intermediate tube for image projection, switchable The intermediate tube for image projection (3-32/1) can also be attached to the new Axioskop 2 / Axioskop 2 routine microscopes via adapter (3-32/2).

  • Page 117: Quantitative Microscopy

    Quantitative microscopy 3.7.1 Measurement of lengths The measurement of lengths using the Axioskop 2 / Axioskop 2 requires the following, for example: − stage micrometer, positive 5 + 100/100 y, D = 0.17 mm as the object − eyepiece crossline micrometer 10:100, d = 26 mm in the eyepiece An overview of available stage micrometers and eyepiece reticles is given in section 1.7.

  • Page 118: Height Measurement

    OPERATION Quantitative microscopy Axioskop 2 / Axioskop 2 After exchange of the stage micrometer for the specimen to be measured, the measuring distance of interest results from the number of increments of the crossline micrometer (tenth estimated), multiplied by the scale value k’. Example: L = 35.5 x 9.9 µm = 351.5 µm...
  • Page 119 OPERATION Axioskop 2 / Axioskop 2 Quantitative microscopy Example: Upper (3-34/1) and lower side (3-34/2) of a thin section were focused using a dry objective = 1.0). The indicated intervals of the mechanical fine drive are 15.0 and 24.5, which means that d’ = 9.5 µm with an interval of 1 µm.
  • Page 120 OPERATION Axioskop 2 / Axioskop 2 3-54 B 40-075 e 06/98...

  • Page 121: Care, Maintenance, Troubleshooting And Service

    CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 2 / Axioskop 2 Contents / List of illustrations CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Contents CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE ........4-3 Instrument care .......................4-3 Instrument maintenance ....................4-4 4.2.1 Performing checks ......................4-4 4.2.2 Changing the fuses......................4-4 4.2.3...
  • Page 122 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 2 / Axioskop 2 B 40-075 e 06/98...

  • Page 123: Instrument Care

    CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Instrument care Care of the Axioskop 2 / Axioskop 2 is limited to the following operations: • Switch off the instrument after every use and place instrument cover on it to protect it from dust and humidity.

  • Page 124: Instrument Maintenance

    CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Instrument maintenance Axioskop 2 / Axioskop 2 Instrument maintenance 4.2.1 Performing checks • Make sure that the instrument(s) comply with the required line voltage (e.g. the HBO 100 transformer). • Check line cable and plug for defects.

  • Page 125: Check Instrument Voltage And Change It, If Required

    Check instrument voltage and change it, if required Axioskop 2 microscope stand The power supply in the Axioskop 2 / Axioskop 2 microscope is suitable for the entire line voltage range from 100 ... 240 V AC (line frequency: 50 - 60 Hz). Separate setting of the instrument voltage is therefore not required.

  • Page 126: Troubleshooting

    CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Troubleshooting Axioskop 2 / Axioskop 2 Troubleshooting Problem Cause Remedy Shadows or inhomogeneous The vis/fot pushrod on the camera Move the vis/fot pushrod on the tube is not in the correct phototube in the correct position image brightness in the field of (intermediate) position.
  • Page 127 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 2 / Axioskop 2 Troubleshooting Problem Cause Remedy Low resolving power and poor Aperture diaphragm opening not Set aperture diaphragm opening image contrast. set correctly. in accordance with the 2/3 rule or depending on the specimen features, see p.
  • Page 128 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Troubleshooting Axioskop 2 / Axioskop 2 Problem Cause Remedy Great focus differences after Focusing eyepieces are not set Set focusing eyepieces to the objective change. correctly. appropriate ametropia, s. p. 2-5. Objective not screwed in until Screw in objective until stop.
  • Page 129 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 2 / Axioskop 2 Troubleshooting Problem Cause Remedy The 12 V 100 W halogen lamp Line cable not connected to the Connect line cable to the line and line. make sure to check the instrument does not function although the and line voltage.

  • Page 130: Spares, Consumables And Tools

    CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Spares, consumables ... Axioskop 2 / Axioskop 2 Spares, consumables and tools Description Cat. No. Application 12 V 100 W halogen lamp 380079 9540 for HAL 100 illuminator HBO 103 W/2 mercury pressure short-arc 380301 9350...

  • Page 131: Requesting Service

    To ensure the optimum setting and trouble-free function of your microscope even for a longer period of time, we would recommend you to conclude a service/maintenance contract with Carl Zeiss. In the case of subsequent orders or when service is required, please get in touch with your local Zeiss agency.
  • Page 132 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 2 / Axioskop 2 4-12 B 40-075 e 06/98...
  • Page 133 ANNEX Axioskop 2 / Axioskop 2 Overview ANNEX List of abbreviations .......................... A-3 List of key words..........................A-5 Certification in accordance with DIN ISO 9001 / EN 46001..............A-9 EC conformity declaration ........................A-11 B 40-075 e 06/98...

  • Page 134: Annex

    ANNEX Axioskop 2 / Axioskop 2 B 40-075 e 06/98...

  • Page 135: List Of Abbreviations

    ANNEX Axioskop 2 / Axioskop 2 List of abbreviations List of abbreviations Alternating Current A-Plan Achromatic objectives featuring improved image flatness (ICS line) suitable for eyeglass wearers Charge Couple Device cod. coded Canadian Standards Association cover slip thickness darkfield diameter (e.g. of filters)
  • Page 136 ANNEX List of abbreviations Axioskop 2 / Axioskop 2 Infinity Color-Corrected System International Electrotechnical Commission International Protection (protection class) International Organization for Standardization measuring distance (length measurement) Light Emitting Diode Microscope Camera / mot. motorized refractive index of the medium...
  • Page 137 ANNEX Axioskop 2 / Axioskop 2 List of key words List of key words Page adapter ............................2-27 adjusting aid............................3-6 ambient conditions .......................... 1-16 ametropia............................2-5 analyzer.........................3-24, 3-31, 3-35 analyzer module ......................3-9, 3-32, 3-35 aperture diaphragm ................ 1-3, 3-5, 3-8, 3-12, 3-25, 3-26, 3-40 automatic mode ........................3-18, 3-20...

  • Page 138: Eyepieces

    ANNEX List of key words Axioskop 2 / Axioskop 2 Page epi-fluorescence ........................1-2, 3-39 eyepiece reticle..........................2-5 eyepieces ......................2-3, 2-4, 2-5, 2-10, 3-6 fast stage lifting........................3-11, 3-14 fast stage lowering ......................3-11, 3-13, 3-22 filters ......................... 3-5, 3-7, 3-9, 3-16 filter mount......................
  • Page 139 ANNEX Axioskop 2 / Axioskop 2 List of key words Page KÖHLER...........................3-9, 3-24, 3-37 lamp voltage..........................3-6, 3-11 light intensity..........................3-6, 3-11 light manager function ........................3-20 light sources ............................ 1-17 line connection ..........................2-27 line voltage............................1-17 luminous-field diaphragm ................ 3-5, 3-6, 3-9, 3-25, 3-30, 3-40 maintenance............................4-4...
  • Page 140 ANNEX List of key words Axioskop 2 / Axioskop 2 Page quantitative microscopy ........................3-51 reflector turret..................1-3, 1-18, 3-9, 3-13, 3-20, 3-22 remedy.............................. 4-6 requesting service..........................4-11 SÉNARMONT polarizer................. 2-18, 3-31, 3-32, 3-36, 3-37 Set key ..................3-12, 3-14, 3-19, 3-20, 3-22, 3-23, 3-26 smoothness.............................

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