Microscopy In Transmitted-Light Brightfield In A Few Steps - Zeiss Axiovert 200 Operating Manual

Carl Zeiss

Microscopy in transmitted-light brightfield in a few steps

Before starting to use the Axiovert 200, make sure to read the notes on instrument safety and
the chapters entitled "Instrument Description" (Chapter 1) and "Start-up" (Chapter 2).
Make the microscope ready for operation as described in chapter 2 and switch it on via the On/Off
switch (0-2/1).
Select the objective with the lowest magnification (e.g. 10x) on the nosepiece (0-2/2). Set factor 1x on
the setting wheel (0-2/4) of the Optovar turret.
Open the luminous-field diaphragm or the aperture diaphragm completely by pulling lever (0-2/16) to
the front until stop or by turning the setting wheel (0-2/20) to the front until stop.
Turn the setting ring (0-2/19) to move the condenser turret in position H for brightfield (or DIC).
Move reflector turret (0-2/5, if available) into the position without filter combination via the setting
ring.
If required, remove analyzer slider (0-2/3) or switch to free light path.
Turn setting wheel for Sideport right / left / vis (0-2/22) to position 100 % vis (visual
Turn setting knob for Frontport / Baseport / vis (0-2/23) to position 100 % vis (
Set beam splitting ratio to 100 % vis (0-2/10) on the tube. Switch off the Bertrand lens (if available).
Move combined rotary / slider knob (0-2/9) to position 100 % vis (
Place a high-contrast specimen on the microscope stage (0-2/21). Adjust the binocular component.
Use the coarse / fine focusing drive (0-2/6) to focus on the selected detail of the specimen. Should no
light be visible in the eyepieces, switch on the halogen illuminator via the HAL on / off switch (0-2/7).
Fig. 0-1 Diaphragm settings in transmitted-
light brightfield according to
KÖHLER
Center (0-1/C) luminous-field diaphragm via the centering screws (0-2/15 and 18) and open it until
the edge of the diaphragm just disappears from the field of view (0-1/D).
Remove one eyepiece from the eyepiece tube (or swing in Bertrand lens) and set aperture diaphragm
(0-2/20) to approx. 2/3 of the diameter of the objective exit pupil (0-1/E). Optimum contrast setting is
dependent on the respective specimen.
Insert the eyepiece again (or swing out Bertrand lens) and refocus, if required, via the fine drive.
After the microscope has been set to transmitted-light brightfield in this way, changing to this special
contrasting technique is now possible (see chapter 3 of this manual).
0-12
INTRODUCTION
Microscopy in transmitted-light brightfield in a few steps
).
Use the toggle switch (0-2/8) to set the light
intensity to comfortable brightness.
Close luminous-field diaphragm (0-2/16) until it
is visible in the field of view, even if not in focus
(0-1/A).
Focus on the edge of the luminous-field
diaphragm (0-1/B) by moving the condenser
(0-2/17) vertically.
Axiovert 200
).
VIS
).
B 40-080 e 03/01