Table of Contents
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Imperial College
Facility for Imaging
by Light Microscopy
Observing Life As It Happens
Startup procedure ................................................................................................................ 2
Adjusting brightfield Köhler illumination ............................................................................... 2
Observing fluorescence through the eyepiece ..................................................................... 3
Acquiring images with the Improvision Volocity software ..................................................... 3
General informations ................................................................................................................................. 3
Acquiring the image ...................................................................................................................... 3
refocussing/ re-centring: ........................................................................................................................... 3
adjusting channel presets: ........................................................................................................................ 4
multidimensional acquisition setup: .......................................................................................................... 4
Shutdown procedure ............................................................................................................ 5
Important additional information ........................................................................................... 7
Volocity Video Toolbar .................................................................................................................. 7
Auto Exposure .............................................................................................................................. 8
Auto Contrast ................................................................................................................................ 8
Keypad controls: ........................................................................................................................... 8
Control buttons on the lefthand side of the microscope: ............................................................... 8
File formats ................................................................................................................................... 8
merge colours: .............................................................................................................................. 8
London
Q
S
UICK
TART
W
IDEFIELD
Z
A
EISS
XIOVERT
(SAFB 409)
18 Feb 2011,<qsg-wf1> p. 1/8
G
:
UIDE
1
200 / V
OLOCITY
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Table of Contents
Related Manuals for Zeiss AXIOVERT 200
Summary of Contents for Zeiss AXIOVERT 200
- Page 1 Imperial College London Facility for Imaging UICK TART UIDE by Light Microscopy IDEFIELD 200 / V EISS XIOVERT OLOCITY (SAFB 409) Observing Life As It Happens Startup procedure ........................ 2 Adjusting brightfield Köhler illumination ................2 Observing fluorescence through the eyepiece ..............3 ...
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Page 2: Startup Procedure
Startup procedure Turn on (in this order): • if required: heater unit and heater, 4 hours before live imaging to allow focus to stabilise: • plate holder • chamber • if required: CO supply • switch on the mercury fluorescence lamp •... -
Page 3: Observing Fluorescence Through The Eyepiece
Observing fluorescence through the eyepiece There are 2 options to observe fluorescence: the common way would be to use the filter cubes in the cube revolver; for some applications the use of a dichroic mirror in the filter cube position together with the filters in the filter wheels is necessary. -
Page 4: Adjusting Channel Presets
Z position button, choose "Set Bottom" in "Acquisition Setup" dialogue • set "Change focus using" to "Zeiss Focus Drive" • choose and set Z-spacing or number of slices o using Autofocus 18 Feb 2011,<qsg-wf1> p. 4/8... -
Page 5: Shutdown Procedure
"Acquisition Setup" dialogue • set "Change focus using" to "Zeiss Focus Drive" • choose and set Z-spacing or number of slices • set "Auto-focus using" to "Zeiss Focus Drive" • choose the lightpath where the Autofocus should take its cues from o set time series o in "Acquisition Setup"... - Page 6 If someone’s booked within two hours: • update usage in Sharepoint • remove your samples • clean objective lenses with fresh lens tissue and close incubation chamber • clear up the desk • save files onto the server or on a mobile harddrive •...
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Page 7: Important Additional Information
Important additional information Volocity Video Toolbar 18 Feb 2011,<qsg-wf1> p. 7/8... -
Page 8: Auto Exposure
Auto Exposure • sets max pixels to 80% (not 100%) (can also be applied to a selected area) Auto Contrast • unlike Gain only affects appearance of the image on the screen(!!!) Keypad controls: • top level button 1 = transmitted light on/off (#2-4 = empty) •...
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