Zeiss Standard 25 ICS Operating Manual
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Zeiss Standard 25 ICS Operating Manual

Zeiss Standard 25 ICS Operating Manual

Transmitted light microscope
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Standard 25
ICS
Transmitted Light
Microscope
Operating Manual

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Summary of Contents for Zeiss Standard 25 ICS

  • Page 1 Standard 25 Transmitted Light Microscope Operating Manual...
  • Page 2 Violations will entail an obligation to pay compensation. All rights reserved in the event of granting of patents or registration of a utility model. Issued by: Carl Zeiss Mikroskopie D-07740 Jena Telephone: (**49) 03641 / 64-1616...

  • Page 3: Table Of Contents

    Standard 25 CONTENTS Page INTRODUCTION........................I Title page ........................... I Copyright..........................II Contents ...........................III Notes on instrument safety ....................... IV Warranty notes ........................VI Overall view ..........................VII DESCRIPTION........................1-3 Name and intended application ....................1-3 Instrument description and main features................1-3 Microscope configurations and modules .................
  • Page 4 Standard 25 OPERATION.......................... 3-3 Switch on the instrument ....................... 3-3 Setting of transmitted-light brightfield for KÖHLER illumination..........3-4 Setting of transmitted-light phase contrast ................3-6 Setting of transmitted-light darkfield ..................3-8 Setting of transmitted light polarization contrast..............3-9 Measurement of lengths ...................... 3-10 Attachment of photomicrography equipment ...............

  • Page 5: Notes On Instrument Safety

    Standard 25 Notes on instrument safety The Standard 25 microscope was designed, produced and tested in compliance with DIN 61010-1 (IEC 1010-1), Safety requirements for electrical measuring, control and laboratory instruments, and meets the requirements of appendix I of directive 73/23/EC and the relevant CSA and UL directives. The microscope meets the requirements of the EC directive 89/336/EC and the EMC legislation of November 9, 1992.
  • Page 6 Standard 25 The Standard 25 microscope, including its original accessories, may only be used for the microscope techniques described in this manual. Particular attention must be paid to the following warning notes: The Standard 25 microscope is categorized as Protection Class I and has been allocated the protection degree IP 20.
  • Page 7 With the exception of the work specified in this manual, no maintenance or repair of the Standard 25 may be performed. Repairs may only be performed by Zeiss service staff or specially authorized personnel. Should any defect occur with the instrument, please get in touch with your local Zeiss agency first.

  • Page 8: Overall View

    Standard 25 Overall view of the Standard 25 microscope with polarization equipment VIII B 40-029 e 01/98...
  • Page 9 Standard 25 DESCRIPTION Contents DESCRIPTION .........................1-3 Name and intended application..................1-3 Instrument description and main features.................1-3 Microscope configurations and modules................1-4 Objectives........................1-6 Eyepieces.........................1-8 Stage micrometers and eyepiece reticles................1-8 Technical Data ....................... 1-10 List of illustrations Fig. 1-1 Microscope modules......................1-2 Fig. 1-2 Standard 25 microscope configurations...............1-4 B 40-029 e 01/98...
  • Page 10 Standard 25 Fig. 1-1 Microscope modules 1 Eyepieces 5 Condenser carrier with condenser 2 Binocular tube 6 Mechanical stage with specimen holder 3 Stand 7 Objective 4 Coaxial coarse and fine drive 8 Objective nosepiece B 40-029 e 01/98...

  • Page 11: Name And Intended Application

    Standard 25 DESCRIPTION Name and intended application Manufacturer's name: Standard 25 ICS microscope The Standard 25 microscope is a universal microscope for the visualization of fine structures and forms in biology and medicine. These are typical application fields of the Standard 25...

  • Page 12: Microscope Configurations And Modules

    Standard 25 Microscope configurations and modules Fig. 1-2 Standard 25 microscope configurations with polarization equipment B 40-029 e 01/98...
  • Page 13 Standard 25 Cat. No. Configurations 490841 9804 Standard 25 binocular microscope for transmitted-light brightfield and 490841 9904 phase contrast using CP Achromat objectives 10x, 40x Ph2 and 100x 490845 9804 "Standard 25 " binocular microscope for transmitted-light brightfield 490845 9904 using objectives "A-Plan"...

  • Page 14: Objectives

    CP ACHROMAT 10x/0.25 where objective magnification, with a defined color ring on the objective being allocated to each magnification step (Zeiss color code) 0.25 numerical aperture infinite tube length can be used with cover slip thickness D = 0 or 0.17 0.17...
  • Page 15 Standard 25 The following objectives are available for the Standard 25 microscope: Microscopy Objective Magnification/Num. Free working Cover slip Cat.No. Technique Aperture distance in mm thickness D in CP-Achromat Transm.-light brightfield CP-Achromat 5x/0.12 11.2 440920 CP-Achromat 10x/0.25 440930 CP-Achromat 40x/0.65 0.17 440950 CP-Achromat...

  • Page 16: Eyepieces

    Standard 25 Eyepieces The following eyepieces are offered for the Standard 25 ICS: Eyepiece Image Angle Cat. No. Eyepiece PL 10x/18 Br. 39° 444131 9901 Eyepiece PL 10x/18 Br. foc. 39° 444132 9901 Eyepiece E-PL 10x/20 Br. 43° 444231 9901 Eyepiece E-PL 10x/20 Br.
  • Page 17 Standard 25 Crossline disk / d = 26 mm 474064 Crossline micrometer disk 10:100 / d =26 474066 9901 gradation length=10 mm increments = 0.1mm gradation tolerance 0.001 mm Net micrometer 12.5x12.5/5;10 / d = 26 mm 474068 area 12.5x12.5 mm, divided in fields of 5x5 or 10x10.

  • Page 18: Technical Data

    Standard 25 Technical Data Dimensions (width x depth x height) Stand with binocular tube................approx. 200 x 350 x 450 mm Stand with phototube ................approx. 200 x 375 x 460 mm Overall height including T2 adapter and CONTAX 167 MT camera housing..... approx. 570 mm Weight Standard 25 with binocular tube..................

  • Page 19: Objectives

    Standard 25 Fuses according IEC 127: for 230 V......................T 0.2 A; 250 V; 5 x 20 mm for 115 V......................T 0.4 A; 250 V; 5 x 20 mm Light source Halogen lamp ........................HAL 6 V 20 W Adjustment of the light source ..............continuous, 1.5 to 6 V DC Color temperature at 6 V ......................
  • Page 20 Standard 25 Binocular phototube 35°/20 maximum field number ......................20 interpupillary distance ............can be set between 55 and 75 mm viewing angle......................... 35° viewing height ....................approx. 430 mm visual port......................tube factor 1x camera/video port ....................tube factor 1x camera/video port ....................

  • Page 21: Start-Up

    Standard 25 START-UP Contents START-UP........................2-3 Unpacking the instrument....................2-3 Attachment of binocular tube 45°/20 ICS or binocular phototube 35°/20 ICS....2-3 Screwing-in of objectives ....................2-3 Insertion of eyepieces ......................2-4 2.4.1 Insertion of eyepiece reticle....................2-4 2.4.2 Compensation of ametropia when eyepiece reticles are used ...........2-5 Setting of interpupillary distance and viewing height ............2-5 Attachment of specimen holder to mechanical stage 75x30 R...........2-6 Attachment of condensers ....................2-7...
  • Page 22 Standard 25 B 40-029 e 01/98...

  • Page 23: Unpacking The Instrument

    Standard 25 START-UP Standard microscope, including accessories, is delivered in standard packaging. We would recommend you to keep the packaging material so that the instrument can be stored for a longer period of time or returned to the manufacturer. Unpacking the instrument Remove the microscope from the transport case and place it on the worktable.

  • Page 24: Insertion Of Eyepieces

    Standard 25 Insertion of eyepieces Remove both protection caps (2-3/1) from the binocular tube (2-3/2). Insert the fixed eyepiece, e.g. Pl 10x/18 Br. (2- 3/3) in the right tube and the focusing eyepiece Pl 10x/18 Br. foc. (2-3/4) in the left tube. The focusing eyepiece is used to compensate for ametropia of the eyes.

  • Page 25: Compensation Of Ametropia When Eyepiece Reticles Are Used

    Standard 25 2.4.2 Compensation of ametropia when eyepiece reticles are used The correct use of an eyepiece reticle requires two focusing eyepieces, e.g. Pl 10x/18 Br. foc., to enable compensation of ametropia. Use the eyelens of the focusing eyepiece to focus on the line figure of the eyepiece reticle; focus on the edge of the field of view if no eyepiece reticle is used.

  • Page 26: Attachment Of Specimen Holder To Mechanical Stage 75X30 R

    Standard 25 Attachment of specimen holder to mechanical stage 75x30 R The Standard 25 features the mechanical stage 75x30 R with ceramic coating (2-6/6). The specimen is precisely moved in x and y using the user-friendly coaxial drive (2-6/4). The x/y gradation on the stage surface (2-6/5) with the two vernier scales helps to relocate certain specimen spots.

  • Page 27: Attachment Of Condensers

    Standard 25 Attachment of condensers 2.7.1 Attach condenser 0.9 Z Unscrew both centering screws (2-7/3) on the condenser carrier (2-7/2) until the condenser 0.9 Z (2-7/9) can be easily inserted in the condenser carrier. Insert condenser 0.9 Z (2-7/9) in the condenser carrier (2-7/2) in such a way that, firstly, the dovetail of the condenser is pressed against spring pin (2-7/1) in the condenser carrier and,...

  • Page 28: Connecting The Instrument To The Line

    Standard 25 Connecting the instrument to the line Check whether the voltage indicated at the rear of the instrument complies with the line voltage! Do not connect the instrument to the line if the instrument voltage and the line voltage are not identical and make sure to inform the nearest CZ agency or CZ service agency.

  • Page 29: Operation

    Standard 25 OPERATION Contents OPERATION ........................3-3 Switch on the instrument....................3-3 Setting of transmitted-light brightfield for KÖHLER illumination ........3-4 Setting of transmitted-light phase contrast ...............3-6 Setting of transmitted-light darkfield................3-8 Setting of transmitted light polarization contrast ..............3-9 Measurement of lengths ....................3-10 Attachment of photomicrography equipment..............
  • Page 30 Standard 25 B 40-029 e 01/98...

  • Page 31: Switch On The Instrument

    Standard 25 OPERATION Switch on the instrument Switch on the instrument via the on/off switch (3-1/3) on the instrument rear. The green LED (3-1/2) must light up. Set the required brightness via the "Brightness" control (3-1/1). Fig. 3-1 Switch on the instrument Depending on the application, place one or several of the following dia.

  • Page 32: Setting Of Transmitted-Light Brightfield For Köhler Illumination

    Standard 25 Setting of transmitted-light brightfield for KÖHLER illumination As described in chapter 2, the microscope is ready for operation and switched on according to section 3.1. If the Ph 2 phase stop carrier (3-3/4) is available on condenser 0.9 Z (3-3/6), it should be swung out of the optical beam path for microscopy in transmitted-light brightfield.
  • Page 33 Standard 25 The KÖHLER illumination principle requires the following settings of the condenser, the luminous-field diaphragm and the aperture diaphragm: Move condenser 0.9 Z (3-3/6) to the upper stop position via the condenser drive, swing condenser front lens 0.9 into the optical beam path and move aperture diaphragm in the center position via lever (3-3/5).

  • Page 34: Setting Of Transmitted-Light Phase Contrast

    Standard 25 Setting of transmitted-light phase contrast The phase contrast technique is mainly used to increase the image contrast of unstained specimens. As described in chapter 2, the microscope is ready for operation and switched on according to section 3.1. The microscope must be set for transmitted-light brightfield, as described in section 3.2.
  • Page 35 Standard 25 Complete phase contrast is available only if the dark phase ring in the objective covers the bright phase stop in the condenser completely and precisely (also see Fig. 3-7/B). To achieve this, the diopter or the centering telescope (3-6/3) must be inserted in tube (3-6/4) instead of an eyepiece.

  • Page 36: Setting Of Transmitted-Light Darkfield

    Standard 25 Setting of transmitted-light darkfield Darkfield is mainly used for small or minute objects such as treponema, spirochaeta, cilia and bacteria, but also for emulsions or unstained objects in watery solutions. The special benefit of the darkfield technique is its natural, true rendition of the original colors (color fidelity).

  • Page 37: Setting Of Transmitted Light Polarization Contrast

    The sliders containing the analyzer and the lambda plate must always be pushed in until stop to make them fully effective and to avoid the field if view to be cut off. Retrofitting of the polarization equipment can be performed by the Zeiss service agency (see section 4.4).

  • Page 38: Measurement Of Lengths

    Standard 25 Measurement of lengths The measurement of lengths using the Standard 25 ICS requires the following, for example: stage micrometer, positive 5 + 100/100 y D = 0.17 mm eyepiece crossline micrometer 10:100, d = 26 mm An overview of available stage micrometers and eyepiece reticles is given in chapter 1.6.

  • Page 39: Attachment Of Photomicrography Equipment

    Standard 25 Attachment of photomicrography equipment The Standard 25 microscope with camera tube can be changed from observation to photography via a pushrod (3-11/7 or 3-12/8) (pushrod pulled out for photomicrography). Special adapters allow commercially available 35 mm SLR cameras and special microscope cameras (e.g. MC 80 ) to be attached to the camera port of the Standard 25 .
  • Page 40 Standard 25 Various T2 adapters for SLR cameras are listed below: T2 adapters to SLR cameras Cat. No. T2 adapter for CONTAX (CONTAX bayonet) 416010 T2 adapter for OLYMPUS OM (OM bayonet) 416002 T2 adapter for MINOLTA (SR bayonet) 416003 T2 adapter for CANON (FD bayonet) 416004 T2 adapter for NIKON (F bayonet)

  • Page 41: Attachment Of Mc 80 Dx Microscope Camera (35 Mm Film Cassette)

    Standard 25 3.7.2 Attachment of MC 80 microscope camera (35 mm film cassette) Insert adapter 60 for microscope camera (3- 12/5) (456006) in camera tube (3-12/6) and fix it using three hexagonal screws (3-12/7). Insert projection lens P 2.5x (3-12/4) in adapter 60 for microscope camera (3-12/5).

  • Page 42: Attachment Of Adapters For Video Cameras

    Standard 25 Attachment of adapters for video cameras The following video adapters and video zoom adapters with 60 mm interface permit the attachment of one-chip b/w and color CCD cameras and three-chip color CCD cameras to the camera tube of the Standard 25 Tube Adapter...
  • Page 43 Standard 25 The connecting piece 60 - 44 also allows video adapters with 44 mm interface to be used with the camera tube of the Standard 25 ICS with 60 mm interface. Video adapters Suitable for:: Comments (Cat.No.) Microscopes with 60 mm interface and all...

  • Page 44: Insertion Of 8X Drawing Eyepiece

    Standard 25 Insertion of 8x drawing eyepiece The 8x drawing eyepiece (444126) is an accessory for microscopic drawing and can only be used in combination with the binocular phototube 35°/20 CS on the Standard 25 The eyepiece contains a ICS. beam splitter which allows the simultaneous observation of the microscope image and the drawing area.

  • Page 45: Care, Maintenance And Troubleshooting

    Standard 25 CARE, MAINTENANCE AND TROUBLESHOOTING Contents CARE, MAINTENANCE AND TROUBLESHOOTING ............4-3 Care and maintenance of the instrument .................4-3 Troubleshooting ......................4-4 4.2.1 Changing the fuses......................4-4 4.2.2 Changing the 6 V 20 W halogen lamp ................4-5 4.2.3 Troubleshooting table .....................4-6 Table of spares, wearing parts and tools ................ 4-10 Requesting service ......................4-11 List of illustrations Fig.
  • Page 46 Standard 25 B 40-029 e 01/98...

  • Page 47: Care And Maintenance Of The Instrument

    Standard 25 CARE, MAINTENANCE AND TROUBLESHOOTING Care and maintenance of the instrument Maintenance of the Standard 25 is limited to the following operations: Cover the instrument with the dust cover after every use. Do not set up the instrument in a damp room, i.e. max. humidity 85%.

  • Page 48: Troubleshooting

    Standard 25 Troubleshooting Troubleshooting is described in further detail using the following two examples: changing the fuses, and changing the 6 V / 20 W halogen lamp. Further measures are summarized under 4.2.3. 4.2.1 Changing the fuses Fig. 4-1 Changing the fuses Switch off the instrument via the on/off switch (4-1/1) and disconnect earth-contact plug (4-1/4) from the line;...

  • Page 49: Changing The 6 V 20 W Halogen Lamp

    Standard 25 4.2.2 Changing the 6 V 20 W halogen lamp The following procedure is required to exchange the lamp: Switch off lamp supply via on/off switch (4-1/2) and allow lamp to cool down for approx. 15 minutes. Disconnect the earth-contact plug (4-1/4) from the line and remove the flat plug (4-1/3) from the instrument connector (4-1/2).

  • Page 50: Troubleshooting Table

    Standard 25 4.2.3 Troubleshooting table Problem Cause Remedy vignetting or unhomogeneous The pushrod on the camera tube is Move the pushrod in the correct image brightness in the field of not in the correct position position (end position), see pages view (observation/photography) 3-11 and 3-13.
  • Page 51 Standard 25 Problem Cause Remedy Low resolving power and poor Aperture diaphragm opening not Set aperture diaphragm opening image contrast set correctly. in accordance with the 2/3 rule or depending on the specimen features, see page 3-5. Condenser not focused correctly Focus condenser and set and condenser lens 0.9 not condenser lens 0.9 correctly or...
  • Page 52 Standard 25 Problem Cause Remedy Left and right fields of view Interpupillary distance of the Set interpupillary distance cannot be combined into an binocular tube is not set correctly. correctly, see pages 2-5, 3-4 image Focusing eyepieces are not set Set focusing eyepieces to the correctly.
  • Page 53 Standard 25 Problem Cause Remedy The 6 V 20 W halogen lamp End of average life of 6 V 20 W Replace 6 V 20 W halogen lamp, flickers, unstable brightness halogen lamp see page 4-5 Incorrectly installed or broken line Correctly connect line cable or cable replace it, see page 4-4...
  • Page 54 Standard 25 Table of spares, consumables and tools Description Cat. No. Application 6 V 20 W long-life halogen lamp 380079 9690.000 for the integrated Standard 25 illumination SW 3 ball-headed screwdriver 000000 0069.551 to change the tubes Eyepiece eyecup 444801 0000.00 recommended for techniques with a low brightness level in order to suppress light reflection...

  • Page 55: Requesting Service

    To ensure the optimum setting and trouble-free function of your microscope even for a longer period of time, we would recommend you to conclude a service/maintenance contract with Carl Zeiss. In the case of subsequent orders or when service is required, please get in touch with your local Zeiss agency.
  • Page 56 Standard 25 4-12 B 40-029 e 01/98...

  • Page 57: Annex

    Standard 25 ANNEX List of abbreviations ........................A-3 Physical and technical units......................A-4 Certification in accordance with DIN EN ISO 9001 / DIN EN 46001..........A-5 EC conformity declaration ......................A-7 B 40-029 e 01/98...
  • Page 58 Standard 25 B 40-029 e 01/98...

  • Page 59: List Of Abbreviations

    Standard 25 List of abbreviations Alternating Current aspheric A-Plan achromatic objectives featuring improved image flatness (ICS line) suitable for eyeglass wearers Correction Blue (conversion filter) Charge Coupled Device CP-Achromat achromatic objective (ICS line) Canadian Standards Association diameter darkfield, or cover slip thickness Direct Current Deutsches Institut für Normung (German standards association) documentation...

  • Page 60: Physical And Technical Units

    Standard 25 polychlorinated biphenyl phase contrast flatfield right (control on the right of the mechanical stage) Single Lens Reflex protection class wrench opening black-and-white slow-blow (fuse type) television T2-Adapter standard adapter for 35 mm cameras Underwriters Laboratories (US test authority) magnification of the objective association of German electrotechnicians visual...
  • Page 61 Click below to find more Mipaper at www.lcis.com.tw Mipaper at www.lcis.com.tw...

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