Zeiss Axioskop 2 plus Operating Manual
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Zeiss Axioskop 2 plus Operating Manual

Routine microscope
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  • Page 2: Copyright

    INTRODUCTION Axioskop 2 plus Carl Zeiss Copyright Axioskop 2 mot plus Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument.

  • Page 3: Table Of Contents

    CHAPTER 1 INSTRUMENT DESCRIPTION ..................1-2 Name and intended application ..................1-2 Instrument description and main features ..............1-2 Axioskop 2 plus (manual) microscope configurations and modules ........1-4 Axioskop 2 mot plus microscope configurations and modules........1-8 Objectives........................1-12 Eyepieces........................1-14 Stage micrometers and eyepiece reticles ..............1-14 Technical data ......................1-16...

  • Page 4: Contents

    Changing the binocular tube ..................2-26 2.2.11 Attachment of intermediate tube with height adjustment..........2-27 2.2.12 Attachment of adapter for the Axioskop 2 plus ............2-28 2.2.13 Installing and Removing “Push&Click” Modules ............2-29 2.2.14 Changing the filter set in the FL P&C reflector module..........2-30 2.2.15...
  • Page 5 CHAPTER 3 OPERATION .........................3-3 Overview of operation and function controls ..............3-3 3.1.1 Operation and function controls of the Axioskop 2 plus..........3-4 3.1.2 Overview of operation and function controls of the Axioskop 2 mot plus.....3-10 Switching on and basic settings ...................3-18 3.2.1...
  • Page 6 INTRODUCTION Axioskop 2 plus Carl Zeiss Contents Axioskop 2 mot plus Page CHAPTER 4 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE ........4-2 Instrument care ......................4-2 Instrument maintenance....................4-3 4.2.1 Performing checks ......................4-3 4.2.2 Changing the fuses .......................4-3 Troubleshooting ......................4-4 Spares, consumables and tools ..................4-8 Requesting service ......................4-9...

  • Page 7: Microscopy In A Few Steps

    ☞ Please read the instructions regarding instrument safety and the Description (Chapter 1) and Start Up (Chapter 2) chapters before using the Axioskop 2 plus and/or Axioskop 2 mot plus microscopes for the first time. As described in chapter 2, the microscope is ready for operation and switched on according to section 3.3.

  • Page 8: Notes On Instrument Safety

    Axioskop 2 mot plus Notes on instrument safety The Axioskop 2 plus and Axioskop 2 mot plus microscopes have been designed produced and tested in compliance with DIN 61010-1 (IEC 1010-1) and DIN EN 61010-2-101 safety requirements for electrical measuring, control and laboratory instruments.
  • Page 9 Notes on instrument safety Carl Zeiss The Axioskop 2 plus and Axioskop 2 mot plus microscopes, including original accessories, may only be used for the microscope techniques described in this manual. Particular attention must be paid to the following warning notes: The manufacturer cannot assume any liability for any other applications, possibly also involving individual modules or single parts.
  • Page 10 For safety reasons, the stage lowering procedure can be automatically switched off (see page 3-13). The Axioskop 2 plus and Axioskop 2 mot plus microscopes are not equipped with any special devices for protection from substances which are corrosive, potentially infectious, toxic, radioactive or other substances which could be hazardous to health.
  • Page 11 The instruments may only be operated by trained personnel who must be aware of the possible danger involved with microscopy and the relevant application. The Axioskop 2 plus / Axioskop 2 mot plus is a high-precision instrument which can be impaired in its performance or destroyed when handled improperly.

  • Page 12: Notes On Warranty

    With the exception of the work specified in this manual, no maintenance or repair of the Axioskop 2 plus / Axioskop 2 mot plus may be performed. Repairs may only be performed by Zeiss service staff or specially authorized personnel. Should any defect occur with the instrument, please get in touch with your local Zeiss agency.

  • Page 13: Overall View Of Axioskop 2 Plus

    Axioskop 2 plus INTRODUCTION Axioskop 2 mot plus Overall view of the Axioskop 2 plus Carl Zeiss Overall view of the Axioskop 2 plus Eyepieces Binocular tube Stand Incident-light illuminator Transmitted-light illuminator Manual universal condenser Mechanical stage Objectives B 40-075 e...

  • Page 14: Overall View Of Axioskop 2 Mot Plus

    INTRODUCTION Axioskop 2 plus Carl Zeiss Overall view of the Axioskop 2 mot plus Axioskop 2 mot plus Overall view of the Axioskop 2 mot plus Eyepieces Binocular ergotube Stand Incident-light illuminator FL excitation filter wheel Transmitted-light illuminator Motorised universal condenser...
  • Page 15 Contents Instrument Description ....................1-2 Name and intended application..................1-2 Instrument description and main features................1-2 Axioskop 2 plus (manual) microscope configurations and modules ........1-4 Axioskop 2 mot plus microscope configurations and modules ..........1-8 Objectives ........................1-12 Eyepieces .........................1-14 Stage micrometers and eyepiece reticles................1-14 Technical Data .........................1-16...

  • Page 16: Name And Intended Application

    Axioskop 2 plus, such as tubes, intermediate tubes and condensers, are easy to attach. The Axioskop 2 plus is offered as a manual and a motorized / coded version. The basic stand is equipped with either manual (Axioskop 2 plus) or motorized / coded operation and function controls (Axioskop 2 mot plus), but is otherwise identical for both versions.

  • Page 17: Instrument Description

    Instrument description and main features Carl Zeiss Major instrument features of the Axioskop 2 plus (manual): − Stable and sturdy diecast stand with T-shaped base in the pyramid design and integrated DC supply for the HAL 12 V 100 W halogen lamp −...

  • Page 18: Axioskop 2 Plus (Manual) Microscope Configurations And Modules

    INSTRUMENT DESCRIPTION Axioskop 2 plus Carl Zeiss Axioskop 2 plus microscope configurations ... Axioskop 2 mot plus Axioskop 2 plus (manual) microscope configurations and modules Fig. 1-1 Axioskop 2 plus (manual) microscope configurations B 40-075 e 10/03...
  • Page 19 Axioskop 2 plus INSTRUMENT DESCRIPTION Axioskop 2 mot plus Axioskop 2 plus microscope configurations ... Carl Zeiss Key to Fig. 1-1 Stand Eyepiece Binocular tube 30°/23 HBO 100 W transformer HBO 103 illuminator HAL 12 V 100 W halogen lamp...
  • Page 20 Axioskop 2 mot plus Axioskop 2 plus (manual) microscope configurations Cat. No. Configurations The "Axioskop 2 plus" routine microscope is offered in the following different configurations: for transmitted-light brightfield with objectives "A-Plan" 5x, 10x, 20x, 40x and 100x for transmitted-light brightfield with objectives "Achroplan"...
  • Page 21 Intermediate tube with height adjustment for binocular ergotube / phototube 452341 / 452342 452376-0000-000 Adapter for Axioskop 2 plus to allow the use of tubes / intermediate tubes from the Axioplan 2 line 452969-0000-000 Intermediate tube for image projection from the Axioplan 2 line...

  • Page 22: Axioskop 2 Mot Plus Microscope Configurations And Modules

    INSTRUMENT DESCRIPTION Axioskop 2 plus Carl Zeiss Axioskop 2 mot plus microscope configurations ... Axioskop 2 mot plus Axioskop 2 mot plus microscope configurations and modules Fig. 1-2 Axioskop 2 mot plus microscope configurations B 40-075 e 10/03...
  • Page 23 Axioskop 2 plus INSTRUMENT DESCRIPTION Axioskop 2 mot plus Axioskop 2 mot plus microscope configurations ... Carl Zeiss Key to Fig. 1-2: Stand Eyepiece Binocular ergotube 6-25°/23 HBO 100 W transformer HBO 103 illuminator HAL 12 V 100 W halogen illuminator...
  • Page 24 INSTRUMENT DESCRIPTION Axioskop 2 plus Carl Zeiss Axioskop 2 mot plus microscope configurations ... Axioskop 2 mot plus Axioskop 2 mot plus microscope configurations Cat. No. Configurations The "Axioskop 2 mot plus" routine microscope is offered in the following configurations:...
  • Page 25 Axioskop 2 plus INSTRUMENT DESCRIPTION Axioskop 2 mot plus Axioskop 2 mot plus microscope configurations ... Carl Zeiss Axioskop 2 mot plus microscope configurations Cat. No. Modules Polarizers: SENARMONT DIC polarizer 453622-0000-000 Rotary polarizer D 453620-0000-000 Circular polarizer D 453623-0000-000...

  • Page 26: Objectives

    Achroplan 10x/0.25 ∞ 0.17 where 10x : objective magnification, with a defined color ring on the objective being allocated to each magnification step (Zeiss color code) 0.25 : numerical aperture ∞ : infinite image distance; these objectives...

  • Page 27: Objectives

    (do not forget to unlock them again!). The following objectives are ideal for the Axioskop 2 plus and Axioskop 2 mot plus microscopes: Microscopy technique...

  • Page 28: Eyepieces

    Axioskop 2 plus Carl Zeiss Eyepieces / Stage micrometers and ... Axioskop 2 mot plus Eyepieces The following eyepieces are offered for the Axioskop 2 plus and Axioskop 2 mot plus: Eyepiece Image angle Application Cat. No. Eyepiece W-PL 10x/23 Br. foc. (2x) 49.4°...
  • Page 29 Axioskop 2 plus INSTRUMENT DESCRIPTION Axioskop 2 mot plus Stage micrometers and ... Carl Zeiss Illustration Description, Technical Data Cat. No. Eyepiece reticle / d = 26 mm 474064-0000-000 474066-9901-000 Crossline micrometer 10:100, d = 26 mm gradation length = 10 mm increments = 0.1mm...

  • Page 30: Technical Data

    Axioskop 2 plus / mot plus with phototube and HAL 100....approx. 280 mm x 600 mm x 475 mm Axioskop 2 plus / mot plus with phototube and HBO 103 ....approx. 280 mm x 733 mm x 475 mm Axioskop 2 plus / mot plus with ergo-phototube and HAL 100..
  • Page 31 Power consumption when HBO 50 is used................max. 350 VA Fuses in accordance with IEC 127 Axioskop 2 plus microscope stand .................T 4 A/H, 5x20 mm Axioskop 2 mot plus microscope stand ................T 4 A/H, 5x20 mm HBO 50AC transformer mbq52ac-z ................100 V, 127 V: 2x T 4 A ........................
  • Page 32 INSTRUMENT DESCRIPTION Axioskop 2 plus Carl Zeiss Technical data Axioskop 2 mot plus Axioskop 2 plus: Stand with manual stage focusing coarse drive ......................6 mm / rotation Fine drive .................. 0.1 mm / rotation; 1 µm increments lifting range ........................max. 25 mm height stop ....................can be set mechanically...

  • Page 33: Start-Up

    Changing the binocular tube....................2-25 2.2.11 Attachment of intermediate tube with height adjustment ..........2-26 2.2.12 Attachment of adapter for the Axioskop 2 plus ..............2-27 2.2.13 Installing and Removing “Push&Click” Modules ...............2-28 2.2.14 Changing the filter set in the FL P&C reflector module .............2-29 2.2.15...
  • Page 34 Changing the binocular tube ......................2-25 Fig. 2-40 Attachment of intermediate tube with height adjustment .............. 2-26 Fig. 2-41 Attachment of adapter for the Axioskop 2 plus ................2-27 Fig. 2-42 Changing the technique module ....................2-28 Fig. 2-43 Changing the filter set in the P&C FL reflector module ..............2-29 Fig.

  • Page 35: Initial Start-Up

    Carl Zeiss START-UP The Axioskop 2 plus / Axioskop 2 mot plus can be installed, converted and started up by the customer himself. However, it is also possible to have the microscope installed or converted by Zeiss service staff against an extra charge.

  • Page 36: Attachment Of Binocular Tube Or Phototube

    All the binocular tubes included in the microscope configurations listed in section 1.3 or 1.4 can be attached to the Axioskop 2 plus / Axio- skop 2 mot plus as described below. • Loosen hexagonal screw (2-2/3) using the SW3 ball-headed screwdriver.
  • Page 37 Axioskop 2 plus START-UP Axioskop 2 mot plus Initial start-up Carl Zeiss Inserting the eyepiece reticle The eyepieces PL 10x/23 Br. foc. are intended for use with eyepiece reticles. The slight image shift caused by the additional path through the glass is taken into account on...

  • Page 38: Setting Of The Interpupillary Distance Of The Binocular Tube

    START-UP Axioskop 2 plus Carl Zeiss Initial start-up Axioskop 2 mot plus 2.1.4 Setting interpupillary distance of the binocular tube The eyepiece distance is matched to the user's individual interpupillary distance by swinging the eyepiece tubes symmetrically towards one another (2-5/A and (2-5/B).

  • Page 39: Attachment Of Universal Condenser

    Axioskop 2 plus START-UP Axioskop 2 mot plus Initial start-up Carl Zeiss 2.1.7 Attachment of universal condenser • Move stage carrier to the upper stop position via the focusing drive. • Use lever (2-7/8) to fold out the front lens on...

  • Page 40: Connection To The Line

    The HBO 100 W transformer must be connected to the line via its line socket (2-10/2). Fig. 2-9 Axioskop 2 plus (rear) 2.1.9 Switch microscope and HBO 100 W transformer on and off • Switch the microscope on and off using the line switch (2-9/1).

  • Page 41: Hal 100 Halogen Illuminator

    Axioskop 2 plus START-UP Axioskop 2 mot plus Initial start-up Carl Zeiss 2.1.10 HAL 100 halogen illuminator Attachment HAL 100 halogen illuminator • Remove cover from transmitted-light tube (2-11/3). • Insert dovetail 2-12/2) of lamp housing (2-11/1) in transmitted-light tube (2-11/3) on the instrument rear and use the SW 3 ball-headed screwdriver to tighten clamping screw (2-11/2).

  • Page 42: Hbo 103 Illuminator

    START-UP Axioskop 2 plus Carl Zeiss Initial start-up Axioskop 2 mot plus Fine adjustment • Attach illuminator to the stand again and tighten clamping screw (2-11/2). • Remove diffusion disk (3-1/15) and filters (3-1/18), if switched in, from the beam path.
  • Page 43 Axioskop 2 plus START-UP Axioskop 2 mot plus Initial start-up Carl Zeiss • Insert multi-pin plug of the HBO 103 illuminator (2-13/3) into socket (2-14/2) of the HBO 100 W transformer and secure it with captive coupling ring. • Connect line cable to socket (2-14/1) of the HBO 100 W transformer first, then connect it to the line.
  • Page 44 START-UP Axioskop 2 plus Carl Zeiss Initial start-up Axioskop 2 mot plus Fig. 2-17 Focal spots of HBO 103 before Fig. 2-18 Focal spots of HBO 103 after coarse coarse adjustment adjustment ☞ The adjusting screws (2-16/2 and 5) should not be changed during coarse adjustment of the focal spots because they influence the reflector setting in the HBO 103 illuminator.
  • Page 45 Axioskop 2 plus START-UP Axioskop 2 mot plus Initial start-up Carl Zeiss • If required, set knurled knob (2-16/6) and adjusting screw (2-16/4) alternately until both focal spots have the same dimension (see Fig. 2-18). • Use adjusting screws (2-16/1 and 3) to adjust the brighter focal spot in the adjusting circle, e.g. on the left side, in the same way as the focal spot image (2-19).

  • Page 46: Connect Pc / Laptop To The Axioskop 2 Mot Plus Microscope

    START-UP Axioskop 2 plus Carl Zeiss Initial start-up Axioskop 2 mot plus 2.1.12 Connect PC / Laptop to the Axioskop 2 mot plus microscope Connect the PC to the Axioskop 2 mot plus microscope via the RS-232 serial interface (2-21/2) on the instrument rear.

  • Page 47: Attachments And Conversions

    Axioskop 2 plus START-UP Axioskop 2 mot plus Attachments and conversions Carl Zeiss Attachments and conversions ☞ The steps required for attachments to and conversions of the microscope are explained in the following. After this, the respective modules must be reset in a functioning status again.

  • Page 48: Changing The Phase Stop For Phase Contrast Or Darkfield In The Universal Condenser

    START-UP Axioskop 2 plus Carl Zeiss Attachments and conversions Axioskop 2 mot plus • In the case of the motorized condenser (Axioskop 2 mot plus), plug connecting cable in the socket on the left front of the stand (2- 23/1) and tighten screwing ring.

  • Page 49: Changing The Dic Prism In The Universal Condenser

    Axioskop 2 plus START-UP Axioskop 2 mot plus Attachments and conversions Carl Zeiss 2.2.3 Changing the DIC prism in the universal condenser • To loosen the cover on the condenser underside (2-25/1), loosen both grub screws using the SW 2 screwdriver remove cover.

  • Page 50: Attachment Of D Polarizer

    START-UP Axioskop 2 plus Carl Zeiss Attachments and conversions Axioskop 2 mot plus 2.2.5 Attachment of D polarizer • Hold the polarizer (2-27/6) parallel to the bottom of the condenser carrier (2-27/3) and screw in the fastening bolts (2-274) of the...

  • Page 51: Changing The Hal 100 Halogen Lamp

    Axioskop 2 plus START-UP Axioskop 2 mot plus Attachments and conversions Carl Zeiss 2.2.7 Changing the HAL 100 halogen lamp • Switch off the microscope as described in section 2.1.9, remove plug (2-11/5) from 12 V / 100 W socket (2-11/4) and allow the instrument to cool down for approx.
  • Page 52 START-UP Axioskop 2 plus Carl Zeiss Attachments and conversions Axioskop 2 mot plus After expiry of the average operation time of 300 h, the HBO 103 W/2 mercury vapor short-arc lamp must be exchanged. The operating time of the lamp can be checked on...
  • Page 53 Axioskop 2 plus START-UP Axioskop 2 mot plus Attachments and conversions Carl Zeiss • Loosen the fixing screw (2-33/5) on the heat sink (2-33/6) and pull off the heat sink. If the heat sink does not come off (or cannot be...

  • Page 54: Changing The Mechanical Stage

    START-UP Axioskop 2 plus Carl Zeiss Attachments and conversions Axioskop 2 mot plus 2.2.9 Changing the mechanical stage Depending on their type, mechanical stages can be bolted on or may be turned and centred. The range of movement in X direction is 75 mm and in Y direction 50 mm.
  • Page 55 Axioskop 2 plus START-UP Axioskop 2 mot plus Attachments and conversions Carl Zeiss • Press stage to the front against spring pin and lower its back in the stage carrier (2-35/3), then let go. • Tighten screwing cap (2-35/2). Centering the stage...
  • Page 56 START-UP Axioskop 2 plus Carl Zeiss Attachments and conversions Axioskop 2 mot plus Adjusting the travel on ergo-drive On the mechanical stages with ergo-drive, the travel range of the X and Y adjustment can be extended by a max. 15 mm by axial movement of the drive buttons.

  • Page 57: Changing The Binocular Tube

    Axioskop 2 plus START-UP Axioskop 2 mot plus Attachments and conversions Carl Zeiss ☞ To ensure long life of the stage, the scuff from the specimen holders should be removed at regular intervals. Make sure that the scuff does not get into the guiding components for the x- movement.

  • Page 58: Attachment Of Intermediate Tube With Height Adjustment

    START-UP Axioskop 2 plus Carl Zeiss Attachments and conversions Axioskop 2 mot plus 2.2.11 Attachment of intermediate tube with height adjustment The intermediate tube with height adjustment may only be used in combination with a binocular ergotube. When removing the intermediate tube and...

  • Page 59: Attachment Of Adapter For The Axioskop 2 Plus

    Axioskop 2 mot plus Attachments and conversions Carl Zeiss 2.2.12 Attachment of adapter for the Axioskop 2 plus The adapter for the Axioskop 2 plus is used to attach intermediate tubes and/or tubes the Axioplan 2 to the Axioskop 2 plus / Axio- skop 2 mot plus.

  • Page 60: Installing And Removing "Push&Click" Modules

    START-UP Axioskop 2 plus Carl Zeiss Attachments and conversions Axioskop 2 mot plus 2.2.13 Installing and Removing “Push&Click” Modules FL P&C reflector module # 000000-1046-281 Optovar module 1,25x (for transmitted-light only) # 000000-1046-284 Optovar module 1,6x (for transmitted-light only) # 000000-1046-283...

  • Page 61: Changing The Filter Set In The Fl P&C Reflector Module

    Axioskop 2 plus START-UP Axioskop 2 mot plus Attachments and conversions Carl Zeiss 2.2.14 Changing the filter set in the FL P&C reflector module The filter sets for the FL P&C reflector module can be combined and assembled individually by the customer.
  • Page 62 START-UP Axioskop 2 plus Carl Zeiss Attachments and conversions Axioskop 2 mot plus • Now tip up the module half on top (2-45/1) and remove from the retaining elements (2-45/5b) of the bottom module half. • Remove the beam splitter (2-45/2) and spring frame (2-45/3) from the bottom module half.

  • Page 63: Attachment Of Active Coobservation Equipment For 2 Users

    Attachment of active coobservation equipment for 2 users The active coobservation equipment for 2 users can only be attached to a Axioskop 2 plus / Axioskop 2 mot plus microscope stand if the incident-light illuminator (e.g. HBO 103) has been removed.
  • Page 64 START-UP Axioskop 2 plus Carl Zeiss Attachments and conversions Axioskop 2 mot plus Binocular ergotube Binocular tube Dust cap Rear dovetail Socket for power supply Heat protection shield Fixation screw Clamping screw Support point (stand) Power unit Plug Clamping screw...

  • Page 65: Attachment Of Multi-Observation Equipment

    We would recommend you to have assembly of the multi-observation equipment performed by Zeiss service staff (also see 4.5), since careful optical adjustment and centering of the fields of view of the various observation tubes is required. Should you want to perform assembly yourself, please proceed as follows: •...
  • Page 66 START-UP Axioskop 2 plus Carl Zeiss Attachments and conversions Axioskop 2 mot plus Clamping screw Coobservation tube End connector R Clamping screw End connector L Setting screw Dovetail Socket for power supply Connecting piece R Binocular tube Plug Connecting piece L Power unit Fig.

  • Page 67: Attachment Of Intermediate Tube For Image Projection

    Axioplan 2 line. • Use SW 3 ball-headed screwdriver to loosen clamping screw (2-48/8) and remove available tube of the Axioskop 2 plus in an upward direction. • Insert dovetail adapter...
  • Page 68 START-UP Axioskop 2 plus Carl Zeiss Axioskop 2 mot plus 2-36 B 40-075 e 10/03...

  • Page 69: Operation

    Contents OPERATION........................3-3 Overview of operation and function controls ..............3-3 3.1.1 Operation and function controls of the Axioskop 2 plus .............3-4 3.1.2 Overview of operation and function controls of the Axioskop 2 mot plus ......3-10 Switching on and basic settings..................3-18 3.2.1 Axioskop 2 plus........................3-18...
  • Page 70 Diagram of the Michel-Lévy color charts................3-33 Fig. 3-25 Components for transmitted-light polarization on the Axioskop 2 plus......3-35 Fig. 3-26 Components for the transmitted-light DIC technique on the Axioskop 2 plus ....3-38 Fig. 3-27 Components for Epi-fluorescence on the Axioskop 2 plus ..........3-40 Fig. 3-28 Attachment of SLR camera, (e.g.

  • Page 71: Overview Of Operation And Function Controls

    OPERATION Overview of operation and function controls The Axioskop 2 plus / Axioskop 2 mot plus routine microscopes are offered in a manual version and in a version with motorized/coded components. In the "Operation" chapter, the manual setting and operation functions are explained first, and the motorized / coded functions are explained afterwards, where necessary.

  • Page 72: Operation And Function Controls Of The Axioskop 2 Plus

    OPERATION Axioskop 2 plus Carl Zeiss Operation and function controls Axioskop 2 mot plus 3.1.1 Operation and function controls of the Axioskop 2 plus Fig. 3-1 Operation and function controls of the Axioskop 2 plus B 40-075 e 02/01...
  • Page 73 Axioskop 2 plus OPERATION Axioskop 2 mot plus Operation and function controls Carl Zeiss Key to Fig. 3-1: Eyepieces Binocular tube Centering screws for luminous-field diaphragm (incident light) Pushrod for luminous-field diaphragm (incident light) Compartment for filter slider Centering screws for aperture diaphragm (incident light)
  • Page 74 OPERATION Axioskop 2 plus Carl Zeiss Operation and function controls Axioskop 2 mot plus Eyepieces − Both eyepiece versions, E-PL 10x/23 Br. foc. and W-PL 10x/23 Br. foc., enable compensation of ametropia of the user's eyes (see section 2.1.3) and also accept eyepiece reticles (see section 1.7).
  • Page 75 Axioskop 2 plus OPERATION Axioskop 2 mot plus Operation and function controls Carl Zeiss Color temperature key 3200 K − When the color temperature key 3200 K is activated, LED step 9 lights up. The DC voltage 10.5 V is then available for the halogen lamp, which corresponds to the color temperature of 3200 K.

  • Page 76: Fig

    OPERATION Axioskop 2 plus Carl Zeiss Operation and function controls Axioskop 2 mot plus Universal condenser Depending on the version, the achromatic- aplanatic universal condenser (3-2/1) is equipped as follows: − fold-out front lens. − turret disk for: brightfield - without or with DIC I, II, III...

  • Page 77: Fig

    Axioskop 2 plus OPERATION Axioskop 2 mot plus Operation and function controls Carl Zeiss Nosepiece with objectives − features compartment (3-4/1) for compensators − W 0.8“ threaded mount for five objectives, each with compartment for DIC slider (3-4/2) − fast change of objectives by turning the knurled ring of the nosepiece (3-4/3).

  • Page 78: Overview Of Operation And Function Controls Of The Axioskop 2 Mot Plus

    OPERATION Axioskop 2 plus Carl Zeiss Operation and function controls Axioskop 2 mot plus 3.1.2 Overview of operation and function controls of the Axioskop 2 mot plus Fig. 3-6 Operation and function controls of the Axioskop 2 mot plus 3-10...
  • Page 79 Axioskop 2 plus OPERATION Axioskop 2 mot plus Operation and function controls Carl Zeiss Key to Fig. 3-6: Motorized, 8-position FL excitation filter wheel for filter diameter 25 mm in epi-fluorescence (optional) LED lamp voltage array Pilot lamp Control for light intensity...

  • Page 80: Fig

    OPERATION Axioskop 2 plus Carl Zeiss Operation and function controls Axioskop 2 mot plus Motorized universal condenser Depending on the version, the achromatic-aplanatic universal condenser is equipped as follows: − Motorized fold-out front lens. − Motorized turret disk with brightfield insert, darkfield stop and phase stops (centering), DIC prisms combined with iris stop.

  • Page 81: Travel Range Of Stage Focusing (Diagram)

    Axioskop 2 plus OPERATION Axioskop 2 mot plus Operation and function controls Carl Zeiss Coded reflector turret − Accepts the maximum of five technique modules such as the FL P&C reflector module and shutter plate for incident light and optovar module and analyzer module for transmitted-light .

  • Page 82: Fig

    OPERATION Axioskop 2 plus Carl Zeiss Operation and function controls Axioskop 2 mot plus for fast stage lifting − When this key is activated, the stage is moved back to the focus position last stored. In the case of different specimen slide thickness, there is a risk of the specimen and/or the objective being damaged during fast stage lifting.
  • Page 83 Axioskop 2 plus OPERATION Axioskop 2 mot plus Operation and function controls Carl Zeiss Binocular ergo-phototube 6-25°/23 (100/100) The camera port (3-10/1) of the binocular ergo- phototube allows the attachment of SLR-cameras, microscope cameras and video cameras via the relevant adapters. The light can be directed either to the eyepieces or to the attached camera via a pushrod.
  • Page 84 OPERATION Axioskop 2 plus Carl Zeiss Operation and function controls Axioskop 2 mot plus Filter mount The filter mount allows three additional dia. 32mm color filters to be swung in the beam path together or separately. • Insert filter in one of the three filter plates (3-12/1).
  • Page 85 Intermediate tube with height adjustment Attachment of the intermediate tube with height adjustment allows the viewing height of the Axioskop 2 plus to be increased continuously from 60 to 110 mm. This intermediate tube can only be used in combination with the binocular ergotube 6-25°/23...

  • Page 86: Switching On And Basic Settings

    − In the manual mode, the function of the light manager is switched off. Illumination intensity and condenser settings must be made manually, as in the Axioskop 2 plus. Programming mode: − In the programming mode, the user can individually match and store the focusing speeds and focus positions of the various objectives (parfocality).
  • Page 87 Axioskop 2 plus OPERATION Axioskop 2 mot plus Switching on and basic settings Carl Zeiss Overview of function and programming modes: Function modes Programming mode automatic manual • Switch on • Keep key "transmitted light • Keep Set key (3-15/1) on...
  • Page 88 OPERATION Axioskop 2 plus Carl Zeiss Switching on and basic settings Axioskop 2 mot plus Illumination setting in the automatic mode (light manager function) When switched on, the Axioskop 2 mot plus always starts in the automatic mode. The factory-aligned...
  • Page 89 If required, the Axioskop 2 mot plus can be switched to the manual mode. Illumination intensity and condenser functions must then be operated manually, as in the Axioskop 2 plus. The manual mode is set as follows: • Switch off the microscope with the on / off switch (3-1/27).
  • Page 90 OPERATION Axioskop 2 plus Carl Zeiss Switching on and basic settings Axioskop 2 mot plus The parameters for stage focusing are set as follows: • Swing in the dry objective with the highest magnification factor via the nosepiece. • Set reflector turret to transmitted-light position (see 3-1/23).
  • Page 91 Axioskop 2 plus OPERATION Axioskop 2 mot plus Switching on and basic settings Carl Zeiss • Leave the programming mode by shortly pressing the Set key. The end of the programming mode is confirmed by a beep when the Set key is released. The pilot lamp lights up in green again.

  • Page 92: Illumination And Contrasting Techniques

    Axioskop 2 plus − The manual Axioskop 2 plus has been set up as described in chapter 2. − The manual Axioskop 2 plus has been switched on. • Set the image brightness via voltage control (3-17/1) on the microscope stand.
  • Page 93 Fig. 3-17 Microscope settings on the Axioskop 2 plus in transmitted-light • Insert eyepiece in the tube again. brightfield ☞ Since field size and objective aperture change after every objective change, the setting of the luminous-field diaphragm and the aperture diaphragm must be repeated to obtain optimum results.
  • Page 94 • Setting of KÖHLER illumination to be performed in the same way as described in section 3.3.1 (3) for the manual Axioskop 2 plus. Unlike this procedure, however, the luminous-field diaphragm must be fully opened after the correct setting of the condenser. Shortly press the Set key on the instrument rear after the last setting step to store the settings.

  • Page 95: Setting Of Transmitted-Light Darkfield

    This is one of the reasons why even fine structures can be resolved, although they are below the resolving power of the light microscope and appear very bright on a dark background. Transmitted-light darkfield configuration for the manual Axioskop 2 plus and the Axioskop 2 mot plus −...
  • Page 96 OPERATION Axioskop 2 plus Carl Zeiss Illumination and contrasting techniques Axioskop 2 mot plus • Insert eyepiece in the tube again. • If the height of the dark field condenser has been set correctly and sensibly enough, any visible brightening in the field of view can be reduced, and the luminous-field diaphragm image will be almost perfectly in focus.

  • Page 97: Setting Of Transmitted-Light Phase Contrast

    Configurations of the manual Axioskop 2 plus and the Axioskop 2 mot plus − Phase contrast objectives with phase rings Ph 1, Ph 2 or Ph 3 for different average numeric apertures which can also be used in brightfield without any restriction.
  • Page 98 OPERATION Axioskop 2 plus Carl Zeiss Illumination and contrasting techniques Axioskop 2 mot plus Transmitted-light phase contrast settings manual Axioskop 2 plus Axioskop 2 mot plus • Swing phase contrast objective, e.g. Ph 1, in the beam path. • Insert phase stop labelled like the phase contrast objective, e.g.

  • Page 99: Setting Of Transmitted-Light Polarization

    Fig. 3-22 Components for transmitted-light • Swing polarizer (3-22/1) in beam path and polarization on the Axioskop 2 plus position it to 0°, provided that a rotary polarizer is used. • Swing in analyzer module on reflector turret (3-22/3). The field of view now appears dark on account of the crossed polarizers.
  • Page 100 − Analyzer module D in reflector turret − Adjusting specimen for polarization microscopy (453679-0000-000) Setting of Axioskop 2 plus and Axioskop 2 mot plus • Set the microscope as for transmitted-light brightfield (see section 3.3.1 (3)), taking care to ensure that the interpupillary distance of the binocular tube has been correctly set (see section 2.1.3 (2)).
  • Page 101 Axioskop 2 plus OPERATION Axioskop 2 mot plus Illumination and contrasting techniques Carl Zeiss • Turn the mechanical stage with specimen, e.g. an artificial fiber until the specimen displays maximum darkness. The fiber is now parallel to one of the two directions of the crosslines. If pronounced deviations (5°...
  • Page 102 OPERATION Axioskop 2 plus Carl Zeiss Illumination and contrasting techniques Axioskop 2 mot plus Conclusions The grayish white color first occurring in the bright position in the above example corresponds to a path difference of 150 nm, according to the Michel Lévy color chart (3-24).
  • Page 103 (azimuth) of the specimen in relation to the polarizer or analyzer. All transparent specimens feature their specific interference colors in optical anisotropy. Axioskop 2 plus and Axioskop 2 mot plus configuration − Objectives suitable for polarization −...

  • Page 104: Setting Of Transmitted-Light Differential Interference Contrast (Dic)

    − DIC analyzer module in reflector turret − Rotary mechanical stage should be preferred Setting of transmitted-light DIC on Axioskop 2 plus and Axioskop 2 mot plus • Swing in the DIC-suitable objective via the nosepiece (3-26/1). Push the relevant DIC slider (3-26/3) in the compartment on the nosepiece until it click-stops.
  • Page 105 Axioskop 2 plus OPERATION Axioskop 2 mot plus Illumination and contrasting techniques Carl Zeiss • Bring SENARMONT polarizer (3-26/7c), consisting of carrier with λ/4 plate (above) and rotary polarizer (below), in click-stop position below the condenser, unless polarization filters are integrated.
  • Page 106 Reflector turret in the condenser or Analyzer module D polarizer DIC slider SENARMONT polarizer Knurled screw Sliding knob for aperture compensator λ diaphragm Fig. 3-26 Components for the transmitted-light DIC technique on the Axioskop 2 plus 3-38 B 40-075 e 02/01...

  • Page 107: Setting Of Epi-Fluorescence

    2.1.11 by using the adjusting aid. If required, re-adjustment must be performed depending on the operation time. Setting of epi-fluorescence on the Axioskop 2 plus and Axioskop 2 mot plus The first epi-fluorescence setting is considerably facilitated if the Plan-Neofluar objective 20x/0.50 and a specimen featuring pronounced fluorescence is used.
  • Page 108 Fig. 3-27 Components for Epi-fluorescence • Use the two centering screws (3-27/6) to center on the Axioskop 2 plus the luminous-field diaphragm in relation to the edge of the field of view. • Either open the luminous-field diaphragm until it just disappears behind the edge of the field of view or, if there is a risk of specimen bleaching, reduce it until it is visible in the field of view.

  • Page 109: Documentation

    3.4.1 Attachment of photomicrography equipment The Axioskop 2 plus / Axioskop 2 mot plus equipped with a binocular phototube (3-28/5) can be switched from observation to photomicrography (pushrod pulled out) using the pushrod (3-28/7). The special T2 adapter 2.5x and other T2 adapters permit commercially available 35 mm SLR cameras (SLR - Single Lens Reflex) and special microscope cameras (e.g.
  • Page 110 OPERATION Axioskop 2 plus Carl Zeiss Documentation Axioskop 2 mot plus The following T2 adapters are available for SLR cameras: T2 Adapter for SLR cameras Cat. No. T2 Adapter for CONTAX (CONTAX-bayonet) 416010-0000-000 T2 Adapter for OLYMPUS OM (OM-bayonet) 416002-0000-000...

  • Page 111: Attachment Of Videomicroscopy Equipment

    3.4.2 Attachment of videomicroscopy equipment Due to ICS optics from Carl Zeiss, the completely corrected intermediate image falls directly on the target of the video camera. This permits optimum quality of the image transfer, even if high-resolution video cameras are used.

  • Page 112: Attachment Of Adapter For Digital Compact Cameras

    Axioskop 2 mot plus The 60 - 44 adapter also allows video adapters with 44 mm interface to be used with the phototube of the Axioskop 2 plus / Axioskop 2 mot plus with 60 mm interface. Video adapter Suitable for: Comments (Cat.

  • Page 113: Multi-Observation Equipment

    Axioskop 2 plus OPERATION Axioskop 2 mot plus Multi-observation equipment Carl Zeiss Multi-observation equipment 3.5.1 Attachment of active coobservation equipment for 2 users The active coobservation equipment is intended for two users and consists of the following: − Basic body (3-29/3) with two mounts for the binocular tubes −...
  • Page 114 OPERATION Axioskop 2 plus Carl Zeiss Multi-observation equipment Axioskop 2 mot plus Binocular ergotube Binocular tube Basic body Power unit Knob for brightness control Lever for light pointer Fig. 3-29 Active coobservation equipment for 2 users 3-46 B 40-075 e...

  • Page 115: Attachment Of Multi-Observation Equipment

    − Binocular tubes 30°/23 for further viewing stations (3-30/6 and 7, 452927-0000-000) ☞ We would recommend you to have the multi-observation equipment assembled by Carl Zeiss service staff, since careful optical adjustment and centering of the fields of view of the various observation tubes is required.
  • Page 116 OPERATION Axioskop 2 plus Carl Zeiss Multi-observation equipment Axioskop 2 mot plus Knob for brightness control Coobservation tube Basic body End connector L Lever for light pointer Power unit Binocular tube End connector R Connecting piece L Connecting piece R Coobservation tube Fig.

  • Page 117: Drawing Device

    3.6.1 Intermediate tube for image projection, switchable The intermediate tube for image projection (3-31/1) can also be attached to the new Axioskop 2 plus / Axioskop 2 mot plus routine microscopes via adapter (3-31/2). The intermediate tube for image projection...

  • Page 118: Quantitative Microscopy

    Quantitative microscopy 3.7.1 Measurement of lengths The measurement of lengths using the Axioskop 2 plus / Axioskop 2 mot plus requires the following, for example: − stage micrometer, positive 5 + 100/100 y, D = 0.17 mm as the object −...

  • Page 119: Height Measurement

    Axioskop 2 plus OPERATION Axioskop 2 mot plus Quantitative microscopy Carl Zeiss After exchange of the stage micrometer for the specimen to be measured, the measuring distance of interest results from the number of increments of the crossline micrometer (tenth estimated), multiplied by the scale value k’.
  • Page 120 OPERATION Axioskop 2 plus Carl Zeiss Quantitative microscopy Axioskop 2 mot plus Example: Upper (3-33/1) and lower side (3-33/2) of a thin section were focused using a dry objective = 1.0). The indicated intervals of the mechanical fine drive are 15.0 and 24.5, which means that d’ = 9.5 µm with an interval of 1 µm.

  • Page 121: Care, Maintenance, Troubleshooting And Service

    Axioskop 2 plus CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 2 mot plus Contents / List of illustrations Carl Zeiss CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Contents CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE..........4-2 Instrument care........................4-2 Instrument maintenance ....................4-3 4.2.1 Performing checks......................4-3 4.2.2 Changing the fuses ......................4-3 Troubleshooting.........................4-4...

  • Page 122: Instrument Care

    CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Instrument care Care of the Axioskop 2 plus / Axioskop 2 mot plus is limited to the following operations: • Switch off the instrument after every use and place instrument cover on it to protect it from dust and humidity.

  • Page 123: Instrument Maintenance

    Axioskop 2 plus CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 2 mot plus Instrument maintenance Carl Zeiss Instrument maintenance 4.2.1 Performing checks • Make sure that the instrument(s) comply with the required line voltage (e.g. the HBO 100 transformer). • Check line cable and plug for defects.

  • Page 124: Troubleshooting

    CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 2 plus Carl Zeiss Troubleshooting Axioskop 2 mot plus Troubleshooting Problem Cause Remedy Shadows or inhomogeneous The vis/fot pushrod on the camera Move the vis/fot pushrod on the tube is not in the correct...
  • Page 125 Axioskop 2 plus CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 2 mot plus Troubleshooting Carl Zeiss Problem Cause Remedy Low resolving power and poor Aperture diaphragm opening not Set aperture diaphragm opening in image contrast. set correctly. accordance with the 2/3 rule or depending on the specimen features, see p.
  • Page 126 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 2 plus Carl Zeiss Troubleshooting Axioskop 2 mot plus Problem Cause Remedy Great focus differences after Focusing eyepieces are not set Set focusing eyepieces to the objective change. correctly. appropriate ametropia, s. p. 2-5.
  • Page 127 Axioskop 2 plus CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 2 mot plus Troubleshooting Carl Zeiss Problem Cause Remedy The 12 V 100 W halogen lamp Line cable not connected to the Connect line cable to the line and line. make sure to check the instrument does not function although the and line voltage.

  • Page 128: Spares, Consumables And Tools

    CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 2 plus Carl Zeiss Spares, consumables ... Axioskop 2 mot plus Spares, consumables and tools Description Cat. No. Application 12 V 100 W halogen lamp 380079-9540-000 for HAL 100 illuminator HBO 103 W/2 mercury pressure short-arc...

  • Page 129: Requesting Service

    Requesting service All repairs of mechanical, optical or electronic components inside the instrument and of the electrical components of the Axioskop 2 plus / Axioskop 2 mot plus may only be performed by Carl Zeiss service staff or specially authorized personnel.
  • Page 130 CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axioskop 2 plus Carl Zeiss Axioskop 2 mot plus 4-10 B 40-075 e 10/03...

  • Page 131: Annex

    Axioskop 2 plus ANNEX Axioskop 2 mot plus Overview Carl Zeiss ANNEX List of abbreviations..........................A-2 List of key words..........................A-4 Patent rights ............................A-8 B 40-075 e 10/03...

  • Page 132: List Of Abbreviations

    ANNEX Axioskop 2 plus Carl Zeiss List of abbreviations Axioskop 2 mot plus List of abbreviations Alternating Current A-Plan Achromatic objectives featuring improved image flatness (ICS line) suitable for eyeglass wearers Charge Couple Device cod. coded Canadian Standards Association cover slip thickness darkfield diameter (e.g.
  • Page 133 Axioskop 2 plus ANNEX Axioskop 2 mot plus List of abbreviations Carl Zeiss International Electrotechnical Commission International Protection (protection class) International Organization for Standardization measuring distance (length measurement) Light Emitting Diode Microscope Camera / mot. motorized refractive index of the medium...

  • Page 134: List Of Key Words

    ANNEX Axioskop 2 plus Carl Zeiss List of key words Axioskop 2 mot plus List of key words Page Adapter ............................2-27 Adjusting aid ............................3-6 Ambient conditions ..........................1-16 Ametropia ............................2-5 Analyzer ........................3-24, 3-31, 3-35 Analyzer module ....................3-9, 3-13, 3-32, 3-35 Aperture diaphragm ..............

  • Page 135: Eyepieces

    Axioskop 2 plus ANNEX Axioskop 2 mot plus List of key words Carl Zeiss Page Epi-fluorescence ........................1-2, 3-39 Ergo-drive ............................2-24 Eyepiece reticle ...........................2-5 Eyepieces ......................2-3, 2-4, 2-5, 2-10, 3-6 Fast stage lifting ........................3-11, 3-14 Fast stage lowering ......................3-11, 3-13, 3-22 Filter ...........................
  • Page 136 ANNEX Axioskop 2 plus Carl Zeiss List of key words Axioskop 2 mot plus Page Intermediate tube with height adjustment ..................3-17 Interpupillary distance .........................2-6 KÖHLER ........................... 3-9, 3-24, 3-37 Lamp voltage ..........................3-6, 3-11 Light intensity ..........................3-6, 3-11 Light manager function ........................3-20 Light sources ............................1-17...
  • Page 137 Axioskop 2 plus ANNEX Axioskop 2 mot plus List of key words Carl Zeiss Page Polarization ............................3-31 Polarizer ..........................2-18, 3-31 Programming mode ....................... 3-18, 3-19, 3-21 Push&Click module ...........................2-28 Quantitative microscopy ........................3-50 Reflector turret ..................1-3, 1-18, 3-9, 3-13, 3-20, 3-22 Remedy ..............................4-4...

  • Page 138: Patent Rights

    ANNEX Axioskop 2 plus Carl Zeiss Patent rights Axioskop 2 mot plus Patent rights The instruments, instrument components or methods described in this manual are protected by the following patents: DE19828548 US6327080 DE29821694 US6392796 US5015082 JP2532164 B 40-075 e 10/03...

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