Zeiss Axiovert 200 Operating Manual page 85

Axiovert 200
Setting of Z focus factors and parfocality
The matching of focus positions (parfocality) and the adaptation of the focus speed (Z-focus factors) to
the objective magnification are normally only performed when the microscope is used for the first time
or after the change of objectives in the nosepiece.
The parfocality values are automatically saved during setting of the Z focus factors. To correctly save the
parfocality values, the objective with the highest magnification must be used to focus on a specimen
before the Set mode is entered.
Place a specimen on the stage. Swing in dry objective with the highest magnification.
Focus on the specimen via the fine adjustment.
Activate Set mode by pressing key Set LM (for more than 2 seconds).
Press one of the two FOCUS keys.
Change to focus programming is made, and SET FOC SPEED 6 is displayed in the upper line of the LCD
display. The number 6 stands for the focus factor currently set. The set nosepiece position and the
objectives in the nosepiece are shown in the lower LCD line.
Activation of one of the two FOCUS keys permits the focus factor to be changed individually for the
swung-in objective.
Key FOCUS :
Key FOCUS :
Then focus on the specimen again and swing in the objective with the next lowest magnification.
While the nosepiece is being moved to the next position, the focus position and focus speed values set
for the previous objective are saved in the Set mode.
Use the current objective to set the focus speed, focus exactly on the specimen and move the
nosepiece to the next position to store the settings.
Repeat the above settings for all objectives. The last settings should always be made with the oil
immersion objectives.
Finally, the nosepiece must be turned by one further position to permit the values to be saved.
To save the entire setting, exit the Set mode by briefly pressing the Set LM key.
Resetting of Z focus factors and parfocality to default values
Activate Set mode.
Press and hold FOCUS
B 40-080 e 03/01
the drive moves faster
the drive moves slower
and FOCUS keys simultaneously for approx. 1 s until you hear a beep.
OPERATION
Axiovert 200 M Carl Zeiss
3-25