Cleaning The Capillary - Agilent Technologies Revident LC/Q-TOF System User Manual

5 Maintenance

Cleaning the capillary

Cleaning the capillary

Schedule
When you observe decreased sensitivity and decreased signal stability.
Equipment List
Steps
1 Put on clean, powder-free nitrile gloves.
2 Dissolve 1 gram of Alconox Powdered Precision Cleaner in 100 mL of
3 If you use a glass graduated cylinder, cover the end of the ion transport
4 Trim the pipette tip to approximately 4 cm so that the capillary can be
5 Place the ion transport capillary upright in a graduated cylinder and fill with
6 Sonicate the graduated cylinder that contains the ion transport capillary in an
Sonication should be done under a fume hood.
C AU T I O N
7 Rinse the capillary and graduated cylinder several times with de-ionized water.
8 Fill the graduated cylinder with de-ionized water and sonicate for 10 to 15
Agilent Revident LC/Q-TOF System User Guide
• Clean powder-free nitrile gloves
• LC/MS-grade isopropanol
• Alconox powdered precision cleaner
• De-ionized (18 MΩ/cm) water
• 100 mL polypropylene graduated cylinder
• 10 mL polypropylene graduated cylinder
• Two 1 mL pipette tips
de-ionized (18 MΩ-cm) water. This concentration is the recommended
concentration for both manual or ultrasonic cleaning.
capillary with a 1 mL pipette tip to protect the metalized plating.
immersed in the cleaning solution.
Alconox Powdered Precision Cleaner solution.
ultrasonic cleaner for 10 to 15 minutes. Do not clean longer than 15 minutes.
minutes.
105